DOI: 10.1093/genetics/118.4.609. Mutations affecting the tRNA-splicing endonuclease activity of Saccharomyces cerevisiae.

Mutations affecting the tRNA-splicing endonuclease activity of Saccharomyces cerevisiae.

10.1093/genetics/118.4.609

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

Abstract Two unlinked mutations that alter the enzyme activity of tRNA-splicing endonuclease have been identified in yeast. The sen1-1 mutation, which maps on chromosome 12, causes temperature-sensitive growth, reduced in vitro endonuclease activity, and in vivo accumulation of unspliced pre-tRNAs. The sen2-1 mutation does not confer a detectable growth defect, but causes a temperature-dependent reduction of in vitro endonuclease activity. Pre-tRNAs do not accumulate in sen2-1 strains. The in vitro enzyme activities of sen1-1 and sen2-1 complement in extracts from a heterozygous diploid, but fail to complement in mixed extracts from separate sen1-1 and sen2-1 haploid strains. These results suggest a direct role for SEN gene products in the enzymatic removal of introns from tRNA that is distinct from the role of other products known to affect tRNA splicing.

Bibliography

Winey, M., & Culbertson, M. R. (1988). Mutations affecting the tRNA-splicing endonuclease activity of Saccharomyces cerevisiae. Genetics, 118(4), 609â617.

Authors 2

M Winey (first)
M R Culbertson (additional)

References 0 Referenced 70

None

Dates

Type	When
Created	4 years, 7 months ago (Jan. 6, 2021, 12:47 p.m.)
Deposited	4 years, 4 months ago (April 22, 2021, 2:30 p.m.)
Indexed	1 month, 2 weeks ago (July 14, 2025, 11:27 p.m.)
Issued	37 years, 5 months ago (April 1, 1988)
Published	37 years, 5 months ago (April 1, 1988)
Published Online	37 years, 5 months ago (April 1, 1988)
Published Print	37 years, 5 months ago (April 1, 1988)

Funders 0

None

BibTeX

@article{Winey_1988, title={Mutations affecting the tRNA-splicing endonuclease activity of Saccharomyces cerevisiae.}, volume={118}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/118.4.609}, DOI={10.1093/genetics/118.4.609}, number={4}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Winey, M and Culbertson, M R}, year={1988}, month=apr, pages={609–617} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        15
      ]
    ],
    "date-time": "2025-07-15T03:27:09Z",
    "timestamp": 1752550029104
  },
  "reference-count": 0,
  "publisher": "Oxford University Press (OUP)",
  "issue": "4",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1988,
            4,
            1
          ]
        ],
        "date-time": "1988-04-01T00:00:00Z",
        "timestamp": 575856000000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1988,
        4,
        1
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>Two unlinked mutations that alter the enzyme activity of tRNA-splicing endonuclease have been identified in yeast. The sen1-1 mutation, which maps on chromosome 12, causes temperature-sensitive growth, reduced in vitro endonuclease activity, and in vivo accumulation of unspliced pre-tRNAs. The sen2-1 mutation does not confer a detectable growth defect, but causes a temperature-dependent reduction of in vitro endonuclease activity. Pre-tRNAs do not accumulate in sen2-1 strains. The in vitro enzyme activities of sen1-1 and sen2-1 complement in extracts from a heterozygous diploid, but fail to complement in mixed extracts from separate sen1-1 and sen2-1 haploid strains. These results suggest a direct role for SEN gene products in the enzymatic removal of introns from tRNA that is distinct from the role of other products known to affect tRNA splicing.</jats:p>",
  "DOI": "10.1093/genetics/118.4.609",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2021,
        1,
        6
      ]
    ],
    "date-time": "2021-01-06T17:47:55Z",
    "timestamp": 1609955275000
  },
  "page": "609-617",
  "source": "Crossref",
  "is-referenced-by-count": 70,
  "title": "Mutations affecting the tRNA-splicing endonuclease activity of Saccharomyces cerevisiae.",
  "prefix": "10.1093",
  "volume": "118",
  "author": [
    {
      "given": "M",
      "family": "Winey",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "M R",
      "family": "Culbertson",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "286",
  "published-online": {
    "date-parts": [
      [
        1988,
        4,
        1
      ]
    ]
  },
  "container-title": "Genetics",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "http://academic.oup.com/genetics/article-pdf/118/4/609/34455030/genetics0609.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "http://academic.oup.com/genetics/article-pdf/118/4/609/34455030/genetics0609.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        4,
        22
      ]
    ],
    "date-time": "2021-04-22T18:30:09Z",
    "timestamp": 1619116209000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://academic.oup.com/genetics/article/118/4/609/5997535"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1988,
        4,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          1988,
          4,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1093/genetics/118.4.609",
  "relation": {},
  "ISSN": [
    "1943-2631"
  ],
  "subject": [],
  "published-other": {
    "date-parts": [
      [
        1988,
        4,
        1
      ]
    ]
  },
  "published": {
    "date-parts": [
      [
        1988,
        4,
        1
      ]
    ]
  }
}