DOI: 10.1093/genetics/113.1.13. HYPER-RECOMBINING RECIPIENT STRAINS IN BACTERIAL CONJUGATION

HYPER-RECOMBINING RECIPIENT STRAINS IN BACTERIAL CONJUGATION

10.1093/genetics/113.1.13

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

ABSTRACT Using a direct enrichment and screening procedure, mutants of Escherichia coli have been isolated in which recombination frequencies for several intragenic Hfr × F- crosses are significantly higher (twofold to sixfold) than in the parental strains. These hyper-recombination mutations comprised five new mutS - and one new mutL - allele. Together with other known mut - alleles, they were analyzed for effects on intragenic recombination using several types of crosses. Hyper-recombination was found for mutS -, mutL -, mutH (=mutR)- and mutU (=uvrD)-, with the largest effects seen for certain alleles of uvrD; these resulted in over 20-fold excesses in recombinant production for Hfr × F- crosses and F'-chromosome homogenotization. Spontaneous mutator ability was not always correlated with degree of hyper-recombination.

Bibliography

Feinstein, S. I., & Low, K. B. (1986). HYPER-RECOMBINING RECIPIENT STRAINS IN BACTERIAL CONJUGATION. Genetics, 113(1), 13â33.

Authors 2

Sheldon I Feinstein (first)
K Brooks Low (additional)

References 0 Referenced 79

None

Dates

Type	When
Created	4 years, 7 months ago (Jan. 6, 2021, 1:58 a.m.)
Deposited	4 years, 4 months ago (April 22, 2021, 2:20 p.m.)
Indexed	1 year, 7 months ago (Jan. 18, 2024, 12:27 a.m.)
Issued	39 years, 3 months ago (May 1, 1986)
Published	39 years, 3 months ago (May 1, 1986)
Published Online	39 years, 3 months ago (May 1, 1986)
Published Print	39 years, 3 months ago (May 1, 1986)

Funders 0

None

BibTeX

@article{Feinstein_1986, title={HYPER-RECOMBINING RECIPIENT STRAINS IN BACTERIAL CONJUGATION}, volume={113}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/113.1.13}, DOI={10.1093/genetics/113.1.13}, number={1}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Feinstein, Sheldon I and Low, K Brooks}, year={1986}, month=may, pages={13–33} }

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