DOI: 10.1093/genetics/109.2.283. MEIOTIC RECOMBINATION AND SPORULATION IN REPAIR-DEFICIENT STRAINS OF YEAST

MEIOTIC RECOMBINATION AND SPORULATION IN REPAIR-DEFICIENT STRAINS OF YEAST

10.1093/genetics/109.2.283

Crossref journal-article

Oxford University Press (OUP)

Genetics (286)

Abstract

ABSTRACT A genetic system designed to monitor recombination and sporulation in various repair-deficient yeast strains was constructed. Variously heterozygous at seven or eight sites distributed across the genome, the system facilitated sensitive detection of changes in frequency or pattern of meiotic recombination. Ten rad mutants sensitive primarily to UV-irradiation and without terminal blocks in the sporulation process were studied. Seven were defective in excision repair (rad1, rad2, rad3, rad4, rad10, rad14 and rad16), and three were defective in mutagenic repair (rad5, rad9 and rad18). Individually, each mutant displayed behavior consistent with an orthodox meiosis including a wild-type meiotic recombination profile with respect to gene conversion, PMS and intergenic map distances. Accordingly, we conclude that these mutants are without major effect on meiotic heteroduplex formation or correction. However, certain combinations of excision-defective mutants with rad18 exhibited marked ascosporal inviability. Tetraploids homozygous for rad1 and rad18 produce a large proportion of diploid spores containing a recessive lethal.

Bibliography

Dowling, E. L., Maloney, D. H., & Fogel, S. (1985). MEIOTIC RECOMBINATION AND SPORULATION IN REPAIR-DEFICIENT STRAINS OF YEAST. Genetics, 109(2), 283â302.

Authors 3

Elizabeth L Dowling (first)
Daniel H Maloney (additional)
Seymour Fogel (additional)

References 0 Referenced 24

None

Dates

Type	When
Created	4 years, 7 months ago (Jan. 5, 2021, 11:50 p.m.)
Deposited	4 years, 4 months ago (April 22, 2021, 1:46 p.m.)
Indexed	1 year, 5 months ago (Feb. 26, 2024, 1:45 p.m.)
Issued	40 years, 6 months ago (Feb. 1, 1985)
Published	40 years, 6 months ago (Feb. 1, 1985)
Published Online	40 years, 6 months ago (Feb. 1, 1985)
Published Print	40 years, 6 months ago (Feb. 1, 1985)

Funders 0

None

BibTeX

@article{Dowling_1985, title={MEIOTIC RECOMBINATION AND SPORULATION IN REPAIR-DEFICIENT STRAINS OF YEAST}, volume={109}, ISSN={1943-2631}, url={http://dx.doi.org/10.1093/genetics/109.2.283}, DOI={10.1093/genetics/109.2.283}, number={2}, journal={Genetics}, publisher={Oxford University Press (OUP)}, author={Dowling, Elizabeth L and Maloney, Daniel H and Fogel, Seymour}, year={1985}, month=feb, pages={283–302} }

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