DOI: 10.1093/embo-reports/kvf068. Human Asf1 and CAF‐1 interact and synergize in a repair‐coupled nucleosome assembly pathway

Human Asf1 and CAF‐1 interact and synergize in a repair‐coupled nucleosome assembly pathway

10.1093/embo-reports/kvf068

Crossref journal-article

Springer Science and Business Media LLC

EMBO reports (297)

Abstract

The efficient assembly of newly replicated and repaired DNA into chromatin is essential for proper genome function. Based on genetic studies in Saccharomyces cerevisiae, the histone chaperone anti‐silencing function 1 (Asf1) has been implicated in the DNA repair response. Here, the human homologs are shown to function synergistically with human CAF‐1 to assemble nucleosomes during nucleotide excision repair in vitro. Furthermore, we demonstrate that hAsf1 proteins can interact directly with the p60 subunit of hCAF‐1. In contrast to hCAF‐1 p60, the nuclear hAsf1 proteins are not significantly associated with chromatin in cells before or after the induction of DNA damage, nor specifically recruited to damaged DNA during repair in a bead‐linked DNA assay. A model is proposed in which the synergism between hAsf1 and CAF‐1 for nucleosome formation during DNA repair is achieved through a transient physical interaction allowing histone delivery from Asf1 to CAF‐1.

Bibliography

Mello, J. A., SilljÃ©, H. H. W., Roche, D. M. J., Kirschner, D. B., Nigg, E. A., & Almouzni, G. (2002). Human Asf1 and CAFâ1 interact and synergize in a repairâcoupled nucleosome assembly pathway. EMBO Reports, 3(4), 329â334. Portico.

Authors 6

Jill A Mello (first)
Herman H W Silljé (additional)
Danièle M J Roche (additional)
Doris B Kirschner (additional)
Erich A Nigg (additional)
Geneviève Almouzni (additional)

References 21 Referenced 239

10.1016/0092-8674(87)90587-3
10.1016/S1097-2765(01)00150-2
10.1016/S0092-8674(00)80164-6
10.1093/emboj/16.20.6281
10.1101/gad.873201
{'key': 'e_1_2_6_7_1', 'first-page': '24', 'volume-title': 'Chromatin Structure and Gene Expression', 'author': 'Kaufman P.D.', 'year': '2000'} / Chromatin Structure and Gene Expression by Kaufman P.D. (2000)
10.1016/S0092-8674(05)80015-7
10.1101/gad.11.3.345
10.1002/(SICI)1097-0061(19970915)13:11<1029::AID-YEA160>3.0.CO;2-1
10.1074/jbc.273.24.15279
10.1083/jcb.143.3.563
10.1128/MCB.20.4.1206-1218.2000
10.1046/j.1365-2443.2000.00319.x
10.1093/emboj/20.8.2015
10.1016/S0960-9822(01)00140-3
10.1016/S0960-9822(01)00298-6
10.1093/emboj/18.20.5691
10.1093/nar/6.2.689
10.1038/990147
10.1128/MCB.21.19.6574-6584.2001
10.1016/S0092-8674(00)81326-4

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 6:55 p.m.)
Deposited	1 year, 8 months ago (Dec. 18, 2023, 3:53 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 1:51 p.m.)
Issued	23 years, 4 months ago (April 1, 2002)
Published	23 years, 4 months ago (April 1, 2002)
Published Online	23 years, 4 months ago (April 1, 2002)
Published Print	23 years, 4 months ago (April 1, 2002)

Funders 0

None

BibTeX

@article{Mello_2002, title={Human Asf1 and CAF‐1 interact and synergize in a repair‐coupled nucleosome assembly pathway}, volume={3}, ISSN={1469-3178}, url={http://dx.doi.org/10.1093/embo-reports/kvf068}, DOI={10.1093/embo-reports/kvf068}, number={4}, journal={EMBO reports}, publisher={Springer Science and Business Media LLC}, author={Mello, Jill A and Silljé, Herman H W and Roche, Danièle M J and Kirschner, Doris B and Nigg, Erich A and Almouzni, Geneviève}, year={2002}, month=apr, pages={329–334} }

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