DOI: 10.1093/embo-reports/kve234. Electron cryo‐microscopy and image reconstruction of adeno‐associated virus type 2 empty capsids

Electron cryo‐microscopy and image reconstruction of adeno‐associated virus type 2 empty capsids

10.1093/embo-reports/kve234

Crossref journal-article

Springer Science and Business Media LLC

EMBO reports (297)

Abstract

Adeno‐associated virus type 2 empty capsids are composed of three proteins, VP1, VP2 and VP3, which have relative molecular masses of 87, 72 and 62 kDa, respectively, and differ in their N‐terminal amino acid sequences. They have a likely molar ratio of 1:1:8 and occupy symmetrical equivalent positions in an icosahedrally arranged protein shell. We have investigated empty capsids of adeno‐associated virus type 2 by electron cryo‐microscopy and icosahedral image reconstruction. The three‐dimensional map at 1.05 nm resolution showed sets of three elongated spikes surrounding the three‐fold symmetry axes and narrow empty channels at the five‐fold axes. The inside of the capsid superimposed with the previously determined structure of the canine parvovirus (Q. Xie and M.S. Chapman, 1996, J. Mol. Biol., 264, 497–520), whereas the outer surface showed clear discrepancies. Globular structures at the inner surface of the capsid at the two‐fold symmetry axes were identified as possible positions for the N‐terminal extensions of VP1 and VP2.

Bibliography

Kronenberg, S., Kleinschmidt, J. A., & BÃ¶ttcher, B. (2001). Electron cryoâmicroscopy and image reconstruction of adenoâassociated virus type 2 empty capsids. EMBO Reports, 2(11), 997â1002. Portico.

Authors 3

Stephanie Kronenberg (first)
Jürgen A Kleinschmidt (additional)
Bettina Böttcher (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 6:47 p.m.)
Deposited	1 year, 8 months ago (Dec. 18, 2023, 3:56 p.m.)
Indexed	3 weeks, 2 days ago (Aug. 6, 2025, 9:51 a.m.)
Issued	23 years, 9 months ago (Nov. 1, 2001)
Published	23 years, 9 months ago (Nov. 1, 2001)
Published Online	23 years, 9 months ago (Nov. 1, 2001)
Published Print	23 years, 9 months ago (Nov. 1, 2001)

Funders 0

None

BibTeX

@article{Kronenberg_2001, title={Electron cryo‐microscopy and image reconstruction of adeno‐associated virus type 2 empty capsids}, volume={2}, ISSN={1469-3178}, url={http://dx.doi.org/10.1093/embo-reports/kve234}, DOI={10.1093/embo-reports/kve234}, number={11}, journal={EMBO reports}, publisher={Springer Science and Business Media LLC}, author={Kronenberg, Stephanie and Kleinschmidt, Jürgen A and Böttcher, Bettina}, year={2001}, month=nov, pages={997–1002} }

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