DOI: 10.1093/embo-reports/kve134. Human stoned B interacts with AP‐2 and synaptotagmin and facilitates clathrin‐coated vesicle uncoating

Human stoned B interacts with AP‐2 and synaptotagmin and facilitates clathrin‐coated vesicle uncoating

10.1093/embo-reports/kve134

Crossref journal-article

Springer Science and Business Media LLC

EMBO reports (297)

Abstract

Synaptic vesicle biogenesis involves the recycling of synaptic vesicle components by clathrin‐mediated endocytosis from the presynaptic membrane. stoned B, a protein encoded by the stoned locus in Drosophila melanogaster has been shown to regulate vesicle recycling by interacting with synaptotagmin. We report here the identification and characterization of a human homolog of stoned B (hStnB). Human stoned B is a brain‐specific protein which co‐enriches with other endocytic proteins such as AP‐2 in a crude synaptic vesicle fraction and at nerve terminals. A domain with homology to the medium chain of adaptor complexes binds directly to both AP‐2 and synaptotagmin and competes with AP‐2 for the same binding site within synaptotagmin. Finally we show that the μ2 homology domain of hStnB stimulates the uncoating of both clathrin and AP‐2 adaptors from clathrin‐coated vesicles. We hypothesize that hStnB regulates synaptic vesicle recycling by facilitating vesicle uncoating.

Bibliography

Walther, K., Krauss, M., Diril, M. K., Lemke, S., Ricotta, D., HÃ¶ning, S., Kaiser, S., & Haucke, V. (2001). Human stoned B interacts with APâ2 and synaptotagmin and facilitates clathrinâcoated vesicle uncoating. EMBO Reports, 2(7), 634â640. Portico.

Authors 8

Kristin Walther (first)
Michael Krauss (additional)
M Kasim Diril (additional)
Steffen Lemke (additional)
Doris Ricotta (additional)
Stefan Höning (additional)
Stephen Kaiser (additional)
Volker Haucke (additional)

References 31 Referenced 44

10.1074/jbc.274.25.17794
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10.1523/JNEUROSCI.21-04-01218.2001 / J. Neurosci. / Interaction of stoned and synaptotagmin in synaptic vesicle endocytosis by Fergestad T. (2001)
10.1523/JNEUROSCI.19-14-05847.1999 / J. Neurosci. / The stoned proteins regulate synaptic vesicle recycling in the presynaptic terminal by Fergestad T. (1999)
10.1083/jcb.146.4.755
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10.1242/jcs.114.2.353

Dates

Type	When
Created	20 years, 4 months ago (April 20, 2005, 3:57 p.m.)
Deposited	1 year, 8 months ago (Dec. 18, 2023, 3:55 p.m.)
Indexed	3 months ago (May 26, 2025, 2:05 a.m.)
Issued	24 years, 1 month ago (July 1, 2001)
Published	24 years, 1 month ago (July 1, 2001)
Published Online	24 years, 1 month ago (July 1, 2001)
Published Print	24 years, 1 month ago (July 1, 2001)

Funders 0

None

BibTeX

@article{Walther_2001, title={Human stoned B interacts with AP‐2 and synaptotagmin and facilitates clathrin‐coated vesicle uncoating}, volume={2}, ISSN={1469-3178}, url={http://dx.doi.org/10.1093/embo-reports/kve134}, DOI={10.1093/embo-reports/kve134}, number={7}, journal={EMBO reports}, publisher={Springer Science and Business Media LLC}, author={Walther, Kristin and Krauss, Michael and Diril, M Kasim and Lemke, Steffen and Ricotta, Doris and Höning, Stefan and Kaiser, Stephen and Haucke, Volker}, year={2001}, month=jul, pages={634–640} }

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