DOI: 10.1093/embo-reports/kvd067. Efficient membrane assembly of the KcsA potassium channel in <i>Escherichia coli</i> requires the protonmotive force

Efficient membrane assembly of the KcsA potassium channel in Escherichia coli requires the protonmotive force

10.1093/embo-reports/kvd067

Crossref journal-article

Springer Science and Business Media LLC

EMBO reports (297)

Abstract

Very little is known about the biogenesis and assembly of oligomeric membrane proteins. In this study, the biogenesis of KcsA, a prokaryotic homotetrameric potassium channel, is investigated. Using in vivo pulse–chase experiments, both the monomeric and tetrameric form could be identified. The conversion of monomers into a tetramer is found to be a highly efficient process that occurs in the Escherichia coli inner membrane. KcsA does not require ATP hydrolysis by SecA for insertion or tetramerization. The presence of the protonmotive force (pmf) is not necessary for transmembrane insertion of KcsA; however, the pmf proved to be essential for the efficiency of oligomerization. From in vivo and in vitro experiments it is concluded that the electrical component, Δψ, is the main determinant for this effect. These results demonstrate a new role of the pmf in membrane protein biogenesis.

Bibliography

van Dalen, A., Schrempf, H., Killian, J. A., & de Kruijff, B. (2000). Efficient membrane assembly of the KcsA potassium channel in Escherichia coli requires the protonmotive force. EMBO Reports, 1(4), 340â346. Portico.

Authors 4

Annemieke van Dalen (first)
Hildgund Schrempf (additional)
J Antoinette Killian (additional)
Ben de Kruijff (additional)

References 33 Referenced 24

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Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 6:48 p.m.)
Deposited	1 year, 8 months ago (Dec. 18, 2023, 3:58 p.m.)
Indexed	1 year, 2 months ago (June 26, 2024, 10:33 a.m.)
Issued	24 years, 11 months ago (Oct. 1, 2000)
Published	24 years, 11 months ago (Oct. 1, 2000)
Published Online	24 years, 11 months ago (Oct. 1, 2000)
Published Print	24 years, 11 months ago (Oct. 1, 2000)

Funders 0

None

BibTeX

@article{van_Dalen_2000, title={Efficient membrane assembly of the KcsA potassium channel in Escherichia coli requires the protonmotive force}, volume={1}, ISSN={1469-3178}, url={http://dx.doi.org/10.1093/embo-reports/kvd067}, DOI={10.1093/embo-reports/kvd067}, number={4}, journal={EMBO reports}, publisher={Springer Science and Business Media LLC}, author={van Dalen, Annemieke and Schrempf, Hildgund and Killian, J Antoinette and de Kruijff, Ben}, year={2000}, month=oct, pages={340–346} }

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