DOI: 10.1093/embo-reports/kvd032. TIF‐IA, the factor mediating growth‐dependent control of ribosomal RNA synthesis, is the mammalian homolog of yeast Rrn3p

TIF‐IA, the factor mediating growth‐dependent control of ribosomal RNA synthesis, is the mammalian homolog of yeast Rrn3p

10.1093/embo-reports/kvd032

Crossref journal-article

Springer Science and Business Media LLC

EMBO reports (297)

Abstract

Cells carefully modulate the rate of rRNA transcription in order to prevent an overinvestment in ribosome synthesis under less favorable nutritional conditions. In mammals, growth‐dependent regulation of RNA polymerase I (Pol I) transcription is mediated by TIF‐IA, an essential initiation factor that is active in extracts from growing but not starved or cycloheximide‐treated mammalian cells. Here we report the molecular cloning and functional characterization of recombinant TIF‐IA, which turns out to be the mammalian homolog of the yeast factor Rrn3p. We demonstrate that TIF‐IA interacts with Pol I in the absence of template DNA, augments Pol I transcription in vivo and rescues transcription in extracts from growth‐arrested cells in vitro.

Bibliography

Bodem, J., Dobreva, G., HoffmannâRohrer, U., Iben, S., Zentgraf, H., Delius, H., Vingron, M., & Grummt, I. (2000). TIFâIA, the factor mediating growthâdependent control of ribosomal RNA synthesis, is the mammalian homolog of yeast Rrn3p. EMBO Reports, 1(2), 171â175. Portico.

Authors 8

Jochen Bodem (first)
Gergana Dobreva (additional)
Urs Hoffmann‐Rohrer (additional)
Sebastian Iben (additional)
Hanswalter Zentgraf (additional)
Hajo Delius (additional)
Martin Vingron (additional)
Ingrid Grummt (additional)

References 13 Referenced 108

10.1128/MCB.14.7.5010
10.1038/sj.onc.1202402
10.1093/nar/13.22.8165
10.1016/S0079-6603(08)60506-1
10.1016/S0021-9258(17)46212-0 / J. Biol. Chem. / Hormonal regulation of transcription of rDNA. Purification and characterization of the hormone‐regulated transcription factor IC by Mahajan P.B. (1990)
10.1093/emboj/17.13.3692
10.1073/pnas.080063997
10.1016/S0076-6879(96)73023-9
10.1002/j.1460-2075.1990.tb07475.x
10.1128/MCB.13.11.6723
10.1016/0092-8674(87)90514-9
10.1002/j.1460-2075.1996.tb00770.x
10.1073/pnas.69.10.2833

Dates

Type	When
Created	23 years, 1 month ago (July 26, 2002, 6:48 p.m.)
Deposited	1 year, 8 months ago (Dec. 18, 2023, 3:58 p.m.)
Indexed	1 month, 2 weeks ago (July 11, 2025, 6:34 a.m.)
Issued	25 years ago (Aug. 1, 2000)
Published	25 years ago (Aug. 1, 2000)
Published Online	25 years ago (Aug. 1, 2000)
Published Print	25 years ago (Aug. 1, 2000)

Funders 0

None

BibTeX

@article{Bodem_2000, title={TIF‐IA, the factor mediating growth‐dependent control of ribosomal RNA synthesis, is the mammalian homolog of yeast Rrn3p}, volume={1}, ISSN={1469-3178}, url={http://dx.doi.org/10.1093/embo-reports/kvd032}, DOI={10.1093/embo-reports/kvd032}, number={2}, journal={EMBO reports}, publisher={Springer Science and Business Media LLC}, author={Bodem, Jochen and Dobreva, Gergana and Hoffmann‐Rohrer, Urs and Iben, Sebastian and Zentgraf, Hanswalter and Delius, Hajo and Vingron, Martin and Grummt, Ingrid}, year={2000}, month=aug, pages={171–175} }

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