DOI: 10.1093/clinchem/42.8.1196. One-step all-in-one dry reagent immunoassays with fluorescent europium chelate label and time-resolved fluorometry

One-step all-in-one dry reagent immunoassays with fluorescent europium chelate label and time-resolved fluorometry

10.1093/clinchem/42.8.1196

Crossref journal-article

Oxford University Press (OUP)

Clinical Chemistry (286)

Abstract

Abstract The availability of an intrinsically fluorescent, inert, and stable Eu chelate label made it feasible to design one-step all-in-one immunoassays with time-resolved fluorometry for detection. Both competitive and noncompetitive immunoassays are performed in microtitration wells containing all assay-specific components in a stable dry form. Only the sample and one assay buffer common for all analytes need to be added. Model assays for human chorionic gonadotropin (hCG), alpha-fetoprotein (AFP), and progesterone all reached equilibrium in 15 min or less without compromising the performance characteristics of the measurements, all of which perform at least equivalent to state-of-the-art assays. The detection limits for hCG, AFP, and progesterone were 0.3 IU/L, 0.1 microgram/L, and 0.5 nmol/L, respectively. The assay ranges for hCG and AFP were linear to 5000 IU/L and 1200 micrograms/L, respectively. The immunoassay format can be readily implemented in a fully automated random-access immunoassay system with optimal performance characteristics and no handling of analyte-specific assay components.

Bibliography

LÃ¶vgren, T., MeriÃ¶, L., Mitrunen, K., MÃ¤kinen, M. L., MÃ¤kelÃ¤, M., Blomberg, K., Palenius, T., & Pettersson, K. (1996). One-step all-in-one dry reagent immunoassays with fluorescent europium chelate label and time-resolved fluorometry. Clinical Chemistry, 42(8), 1196â1201.

Authors 8

T Lövgren (first)
L Meriö (first)
K Mitrunen (first)
M L Mäkinen (first)
M Mäkelä (first)
K Blomberg (first)
T Palenius (first)
K Pettersson (first)

References 0 Referenced 70

None

Dates

Type	When
Created	5 years, 7 months ago (Jan. 11, 2020, 3:50 a.m.)
Deposited	5 years, 6 months ago (March 3, 2020, 2:56 a.m.)
Indexed	2 days, 12 hours ago (Sept. 3, 2025, 6:59 a.m.)
Issued	29 years, 1 month ago (Aug. 1, 1996)
Published	29 years, 1 month ago (Aug. 1, 1996)
Published Online	29 years, 1 month ago (Aug. 1, 1996)
Published Print	29 years, 1 month ago (Aug. 1, 1996)

Funders 0

None

BibTeX

@article{L_vgren_1996, title={One-step all-in-one dry reagent immunoassays with fluorescent europium chelate label and time-resolved fluorometry}, volume={42}, ISSN={1530-8561}, url={http://dx.doi.org/10.1093/clinchem/42.8.1196}, DOI={10.1093/clinchem/42.8.1196}, number={8}, journal={Clinical Chemistry}, publisher={Oxford University Press (OUP)}, author={Lövgren, T and Meriö, L and Mitrunen, K and Mäkinen, M L and Mäkelä, M and Blomberg, K and Palenius, T and Pettersson, K}, year={1996}, month=aug, pages={1196–1201} }

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