DOI: 10.1093/clinchem/31.9.1546. Heterogeneous enzyme immunoassay with electrochemical detection: competitive and "sandwich"-type immunoassays.

Heterogeneous enzyme immunoassay with electrochemical detection: competitive and "sandwich"-type immunoassays.

10.1093/clinchem/31.9.1546

Crossref journal-article

Oxford University Press (OUP)

Clinical Chemistry (286)

Abstract

Abstract In these competitive and "sandwich"-type heterogeneous enzyme immunoassays, based on liquid chromatography with electrochemical detection, rabbit immunoglobulin G is used as a model compound. Alkaline phosphatase (EC 3.1.3.1), the labeling enzyme, catalyzes conversion of phenyl phosphate to phenol. After separation on an octyldecylsilane column, the enzyme-generated phenol is detected in a thin-layer cell at a carbon-paste working electrode. The detection limit for phenol is 5.0 nmol/L. The electrode response varies linearly with concentration over a range of three orders of magnitude. For the sandwich-type assay procedure the detection limit is 10 ng/L; the linearity ranges over four orders of magnitude. The detection limit of the competitive immunoassay is 5 micrograms/L. The dynamic range spans two orders of magnitude.

Bibliography

Wehmeyer, K. R., Halsall, H. B., & Heineman, W. R. (1985). Heterogeneous enzyme immunoassay with electrochemical detection: competitive and âsandwichâ-type immunoassays. Clinical Chemistry, 31(9), 1546â1549.

Authors 3

K R Wehmeyer (first)
H B Halsall (first)
W R Heineman (first)

References 0 Referenced 54

None

Dates

Type	When
Created	5 years, 7 months ago (Jan. 16, 2020, 4:11 a.m.)
Deposited	5 years, 5 months ago (March 4, 2020, 12:52 a.m.)
Indexed	4 months, 2 weeks ago (April 7, 2025, 6:06 p.m.)
Issued	39 years, 11 months ago (Sept. 1, 1985)
Published	39 years, 11 months ago (Sept. 1, 1985)
Published Online	39 years, 11 months ago (Sept. 1, 1985)
Published Print	39 years, 11 months ago (Sept. 1, 1985)

Funders 0

None

BibTeX

@article{Wehmeyer_1985, title={Heterogeneous enzyme immunoassay with electrochemical detection: competitive and “sandwich”-type immunoassays.}, volume={31}, ISSN={1530-8561}, url={http://dx.doi.org/10.1093/clinchem/31.9.1546}, DOI={10.1093/clinchem/31.9.1546}, number={9}, journal={Clinical Chemistry}, publisher={Oxford University Press (OUP)}, author={Wehmeyer, K R and Halsall, H B and Heineman, W R}, year={1985}, month=sep, pages={1546–1549} }

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