DOI: 10.1093/clinchem/21.6.741. Thermochemical Determination of Glucose in Serum, Plasma, and Whole Blood without Prior Deproteinization

Thermochemical Determination of Glucose in Serum, Plasma, and Whole Blood without Prior Deproteinization

10.1093/clinchem/21.6.741

Crossref journal-article

Oxford University Press (OUP)

Clinical Chemistry (286)

Abstract

Abstract Glucose can be determined by phosphorylatlon in the presence of hexokinase (EC 2.7.1.1) by using Mg2+/ (ATP)2- as the phosphorylating agent. A novel instrumental quantitation method is described, direct injection enthalpimetry, whereby the heat of the enzymatically catalyzed reaction was measured. The reaction was allowed to proceed to virtual completion (99.5+%) in an adiabatic Dewar vessel at 25.00 ± 0.01 °C (range). Samples were sequentially injected into a reaction mixture consisting of hexokinase, ATP, Mg2+, and a tris(hydroxymethyl)aminomethane buffer (pH 8). Single analyses required &lt;2 min for 0-3 g/liter samples. The linear dynamic range was 0.3-10 g/liter, with zero intercept and a precision and accuracy of 2%. Electrical calibration in situ obviated the need for chemical standards, and because protein and color do not interfere, no sample pretreatment was necessary before analysis.

Bibliography

McGlothlin, C. D., & Jordan, J. (1975). Thermochemical Determination of Glucose in Serum, Plasma, and Whole Blood without Prior Deproteinization. Clinical Chemistry, 21(6), 741â745.

Authors 2

C David McGlothlin (first)
Joseph Jordan (first)

References 0 Referenced 11

None

Dates

Type	When
Created	5 years, 7 months ago (Jan. 20, 2020, 3:21 a.m.)
Deposited	5 years, 5 months ago (March 5, 2020, 11:27 p.m.)
Indexed	1 year, 6 months ago (Feb. 6, 2024, 9:24 p.m.)
Issued	50 years, 4 months ago (May 1, 1975)
Published	50 years, 4 months ago (May 1, 1975)
Published Online	50 years, 4 months ago (May 1, 1975)
Published Print	50 years, 4 months ago (May 1, 1975)

Funders 0

None

BibTeX

@article{McGlothlin_1975, title={Thermochemical Determination of Glucose in Serum, Plasma, and Whole Blood without Prior Deproteinization}, volume={21}, ISSN={1530-8561}, url={http://dx.doi.org/10.1093/clinchem/21.6.741}, DOI={10.1093/clinchem/21.6.741}, number={6}, journal={Clinical Chemistry}, publisher={Oxford University Press (OUP)}, author={McGlothlin, C David and Jordan, Joseph}, year={1975}, month=may, pages={741–745} }

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