Abstract
Abstract Summary State-of-the-art light and electron microscopes are capable of acquiring large image datasets, but quantitatively evaluating the data often involves manually annotating structures of interest. This process is time-consuming and often a major bottleneck in the evaluation pipeline. To overcome this problem, we have introduced the Trainable Weka Segmentation (TWS), a machine learning tool that leverages a limited number of manual annotations in order to train a classifier and segment the remaining data automatically. In addition, TWS can provide unsupervised segmentation learning schemes (clustering) and can be customized to employ user-designed image features or classifiers. Availability and Implementation TWS is distributed as open-source software as part of the Fiji image processing distribution of ImageJ at http://imagej.net/Trainable_Weka_Segmentation. Supplementary information Supplementary data are available at Bioinformatics online.
References
7
Referenced
1,943
10.1007/978-3-319-28549-8_7
/ Focus on Bio-Image Informatics by Dietz (2016)10.1145/1656274.1656278
/ ACM SIGKDD Explor. Newslett / The weka data mining software: an update by Hall (2009)10.1093/bioinformatics/btr095
/ Bioinformatics / Improved structure, function and compatibility for cellprofiler: modular high-throughput image analysis software by Kamentsky (2011){'key': '2023063012504785400_btx180-B4', 'author': 'Li', 'year': '2015'}
by Li (2015)10.1093/bioinformatics/btw013
/ Bioinformatics / Collaborative analysis of multi-gigapixel imaging data using cytomine by Marée (2016)10.1038/nmeth.2019
/ Nat. Methods / Fiji: an open-source platform for biological-image analysis by Schindelin (2012){'key': '2023063012504785400_btx180-B7', 'author': 'Sommer', 'year': '2011'}
by Sommer (2011)
Dates
Type | When |
---|---|
Created | 8 years, 4 months ago (March 28, 2017, 10:04 a.m.) |
Deposited | 2 years, 1 month ago (June 30, 2023, 8:50 a.m.) |
Indexed | 10 minutes ago (Aug. 27, 2025, 8:07 a.m.) |
Issued | 8 years, 4 months ago (March 30, 2017) |
Published | 8 years, 4 months ago (March 30, 2017) |
Published Online | 8 years, 4 months ago (March 30, 2017) |
Published Print | 8 years ago (Aug. 1, 2017) |
@article{Arganda_Carreras_2017, title={Trainable Weka Segmentation: a machine learning tool for microscopy pixel classification}, volume={33}, ISSN={1367-4811}, url={http://dx.doi.org/10.1093/bioinformatics/btx180}, DOI={10.1093/bioinformatics/btx180}, number={15}, journal={Bioinformatics}, publisher={Oxford University Press (OUP)}, author={Arganda-Carreras, Ignacio and Kaynig, Verena and Rueden, Curtis and Eliceiri, Kevin W and Schindelin, Johannes and Cardona, Albert and Sebastian Seung, H}, editor={Murphy, Robert}, year={2017}, month=mar, pages={2424–2426} }