DOI: 10.1091/mbc.e12-12-0908. β1 integrin regulates Arg to promote invadopodial maturation and matrix degradation

β1 integrin regulates Arg to promote invadopodial maturation and matrix degradation

10.1091/mbc.e12-12-0908

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

β1 integrin has been shown to promote metastasis in a number of tumor models, including breast, ovarian, pancreatic, and skin cancer; however, the mechanism by which it does so is poorly understood. Invasive membrane protrusions called invadopodia are believed to facilitate extracellular matrix degradation and intravasation during metastasis. Previous work showed that β1 integrin localizes to invadopodia, but its role in regulating invadopodial function has not been well characterized. We find that β1 integrin is required for the formation of mature, degradation-competent invadopodia in both two- and three-dimensional matrices but is dispensable for invadopodium precursor formation in metastatic human breast cancer cells. β1 integrin is activated during invadopodium precursor maturation, and forced β1 integrin activation enhances the rate of invadopodial matrix proteolysis. Furthermore, β1 integrin interacts with the tyrosine kinase Arg and stimulates Arg-dependent phosphorylation of cortactin on tyrosine 421. Silencing β1 integrin with small interfering RNA completely abrogates Arg-dependent cortactin phosphorylation and cofilin-dependent barbed-end formation at invadopodia, leading to a significant decrease in the number and stability of mature invadopodia. These results describe a fundamental role for β1 integrin in controlling actin polymerization–dependent invadopodial maturation and matrix degradation in metastatic tumor cells.

Bibliography

Beaty, B. T., Sharma, V. P., Bravo-Cordero, J. J., Simpson, M. A., Eddy, R. J., Koleske, A. J., & Condeelis, J. (2013). Î²1 integrin regulates Arg to promote invadopodial maturation and matrix degradation. Molecular Biology of the Cell, 24(11), 1661â1675.

Authors 7

Brian T. Beaty (first)
Ved P. Sharma (additional)
Jose J. Bravo-Cordero (additional)
Mark A. Simpson (additional)
Robert J. Eddy (additional)
Anthony J. Koleske (additional)
John Condeelis (additional)

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Dates

Type	When
Created	12 years, 5 months ago (April 4, 2013, 3:09 a.m.)
Deposited	3 years, 6 months ago (Feb. 12, 2022, 8:41 p.m.)
Indexed	1 month, 2 weeks ago (July 16, 2025, 7:50 a.m.)
Issued	12 years, 3 months ago (June 1, 2013)
Published	12 years, 3 months ago (June 1, 2013)
Published Print	12 years, 3 months ago (June 1, 2013)

Funders 0

None

BibTeX

@article{Beaty_2013, title={β1 integrin regulates Arg to promote invadopodial maturation and matrix degradation}, volume={24}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e12-12-0908}, DOI={10.1091/mbc.e12-12-0908}, number={11}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Beaty, Brian T. and Sharma, Ved P. and Bravo-Cordero, Jose J. and Simpson, Mark A. and Eddy, Robert J. and Koleske, Anthony J. and Condeelis, John}, editor={Parent, Carole}, year={2013}, month=jun, pages={1661–1675} }

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  "abstract": "<jats:p>\u03b21 integrin has been shown to promote metastasis in a number of tumor models, including breast, ovarian, pancreatic, and skin cancer; however, the mechanism by which it does so is poorly understood. Invasive membrane protrusions called invadopodia are believed to facilitate extracellular matrix degradation and intravasation during metastasis. Previous work showed that \u03b21 integrin localizes to invadopodia, but its role in regulating invadopodial function has not been well characterized. We find that \u03b21 integrin is required for the formation of mature, degradation-competent invadopodia in both two- and three-dimensional matrices but is dispensable for invadopodium precursor formation in metastatic human breast cancer cells. \u03b21 integrin is activated during invadopodium precursor maturation, and forced \u03b21 integrin activation enhances the rate of invadopodial matrix proteolysis. Furthermore, \u03b21 integrin interacts with the tyrosine kinase Arg and stimulates Arg-dependent phosphorylation of cortactin on tyrosine 421. Silencing \u03b21 integrin with small interfering RNA completely abrogates Arg-dependent cortactin phosphorylation and cofilin-dependent barbed-end formation at invadopodia, leading to a significant decrease in the number and stability of mature invadopodia. These results describe a fundamental role for \u03b21 integrin in controlling actin polymerization\u2013dependent invadopodial maturation and matrix degradation in metastatic tumor cells.</jats:p>",
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