DOI: 10.1091/mbc.e09-08-0730. Evolutionary Gain of Function for the ER Membrane Protein Sec62 from Yeast to Humans

Evolutionary Gain of Function for the ER Membrane Protein Sec62 from Yeast to Humans

10.1091/mbc.e09-08-0730

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Because of similarity to their yeast orthologues, the two membrane proteins of the human endoplasmic reticulum (ER) Sec62 and Sec63 are expected to play a role in protein biogenesis in the ER. We characterized interactions between these two proteins as well as the putative interaction of Sec62 with ribosomes. These data provide further evidence for evolutionary conservation of Sec62/Sec63 interaction. In addition, they indicate that in the course of evolution Sec62 of vertebrates has gained an additional function, the ability to interact with the ribosomal tunnel exit and, therefore, to support cotranslational mechanisms such as protein transport into the ER. This view is supported by the observation that Sec62 is associated with ribosomes in human cells. Thus, the human Sec62/Sec63 complex and the human ER membrane protein ERj1 are similar in providing binding sites for BiP in the ER-lumen and binding sites for ribosomes in the cytosol. We propose that these two systems provide similar chaperone functions with respect to different precursor proteins.

Bibliography

MÃ¼ller, L., de Escauriaza, M. D., Lajoie, P., Theis, M., Jung, M., MÃ¼ller, A., Burgard, C., Greiner, M., Snapp, E. L., Dudek, J., & Zimmermann, R. (2010). Evolutionary Gain of Function for the ER Membrane Protein Sec62 from Yeast to Humans. Molecular Biology of the Cell, 21(5), 691â703.

Authors 11

Linda Müller (first)
Maria Diaz de Escauriaza (additional)
Patrick Lajoie (additional)
Melanie Theis (additional)
Martin Jung (additional)
Anika Müller (additional)
Carsten Burgard (additional)
Markus Greiner (additional)
Erik L. Snapp (additional)
Johanna Dudek (additional)
Richard Zimmermann (additional)

References 36 Referenced 84

10.1083/jcb.200409174 / J. Cell Biol by Alder N. N. (2005)
10.1016/S0092-8674(01)00541-4 / Cell by Beckmann R. (2001)
10.1038/nsmb998 / Nat. Struct. Mol. Biol by Blau M. (2005)
10.1038/ng1357 / Nat. Genet by Davila S. (2004)
10.1128/MCB.10.11.6024 / Mol. Cell. Biol by Deshaies R. J. (1990)
10.1002/j.1460-2075.1996.tb01085.x / EMBO J by Dierks T. (1996)
10.1093/emboj/cdf315 / EMBO J by Dudek J. (2002)
10.1038/nsmb1007 / Nat. Struct. Mol. Biol by Dudek J. (2005)
10.1200/JCO.2005.00.695 / J. Clin. Oncol by Eschrich S. (2005)
10.1038/nature02899 / Nature by Ferbitz L. (2004)
10.1016/S0040-4020(01)85612-X / Tetrahedron by Frank R. (1992)
10.1074/jbc.M801499200 / J. Biol. Chem by Goldshmidt H. (2008)
10.1038/sj.embor.7400551 / EMBO Rep by Grallath S. (2006)
10.1038/nature02342 / Nature by Halic M. (2004)
10.1016/S0092-8674(00)81403-8 / Cell by Hamman B. D. (1998)
10.1074/jbc.M511402200 / J. Biol. Chem by Jermy A. J. (2006)
10.1158/1541-7786.MCR-05-0165 / Mol. Cancer Res by Jung V. (2006)
10.1038/sj.emboj.7600731 / EMBO J by Kalies K.-U. (2005)
10.1074/jbc.M310903200 / J. Biol. Chem by Kroczynska B. (2004)
10.1016/j.bbrc.2005.10.101 / Biochem. Biophys. Res. Comm by Kroczynska B. (2005)
10.1074/jbc.275.19.14550 / J. Biol. Chem by Meyer H.-A. (2000)
{'key': 'B22', 'first-page': '3641', 'volume': '62', 'author': 'Mori Y.', 'year': '2002', 'journal-title': 'Cancer Res'} / Cancer Res by Mori Y. (2002)
10.1002/j.1460-2075.1994.tb06859.x / EMBO J by Noel P. J. (1994)
10.1038/42276 / Nature by Plemper R. K. (1997)
10.1016/S0962-8924(00)01905-X / Trends Cell Biol by Potter M. D. (2001)
10.1053/j.gastro.2004.12.051 / Gastroenterology by Schulmann K. (2005)
10.1083/jcb.200312079 / J. Cell Biol by Snapp E. L. (2004)
{'key': 'B28', 'first-page': '1', 'volume-title': 'Ribosomes and Protein Synthesis: A Practical Approach', 'author': 'Spedding G.', 'year': '1990'} / Ribosomes and Protein Synthesis: A Practical Approach by Spedding G. (1990)
10.1073/pnas.97.13.7214 / Proc. Natl. Acad. Sci. USA by Tyedmers J. (2000)
10.1038/sj.embor.embor826 / EMBO Rep by Tyedmers J. (2003)
10.1074/jbc.M511420200 / J. Biol. Chem by Wegrzyn R. D. (2006)
10.1002/yea.954 / Yeast by Willer M. (2003)
10.1091/mbc.11.11.3859
10.1016/S0092-8674(04)00169-2 / Cell by Woolhead C. A. (2004)
10.1016/j.molmed.2006.10.004 / Trends Mol. Med by Zimmermann R. (2006)
10.1016/j.pt.2009.01.002 / Trends Parasitol by Zimmermann R. (2009)

Dates

Type	When
Created	15 years, 7 months ago (Jan. 13, 2010, 4:28 p.m.)
Deposited	6 years, 1 month ago (July 14, 2019, 6:34 a.m.)
Indexed	3 weeks, 6 days ago (July 26, 2025, 5:11 a.m.)
Issued	15 years, 5 months ago (March 1, 2010)
Published	15 years, 5 months ago (March 1, 2010)
Published Print	15 years, 5 months ago (March 1, 2010)

Funders 0

None

BibTeX

@article{M_ller_2010, title={Evolutionary Gain of Function for the ER Membrane Protein Sec62 from Yeast to Humans}, volume={21}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e09-08-0730}, DOI={10.1091/mbc.e09-08-0730}, number={5}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Müller, Linda and de Escauriaza, Maria Diaz and Lajoie, Patrick and Theis, Melanie and Jung, Martin and Müller, Anika and Burgard, Carsten and Greiner, Markus and Snapp, Erik L. and Dudek, Johanna and Zimmermann, Richard}, editor={Hegde, Ramanujan S.}, year={2010}, month=mar, pages={691–703} }

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