DOI: 10.1091/mbc.e08-10-1010. Discrete Determinants in ArfGAP2/3 Conferring Golgi Localization and Regulation by the COPI Coat

Discrete Determinants in ArfGAP2/3 Conferring Golgi Localization and Regulation by the COPI Coat

10.1091/mbc.e08-10-1010

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

From yeast to mammals, two types of GTPase-activating proteins, ArfGAP1 and ArfGAP2/3, control guanosine triphosphate (GTP) hydrolysis on the small G protein ADP-ribosylation factor (Arf) 1 at the Golgi apparatus. Although functionally interchangeable, they display little similarity outside the catalytic GTPase-activating protein (GAP) domain, suggesting differential regulation. ArfGAP1 is controlled by membrane curvature through its amphipathic lipid packing sensor motifs, whereas Golgi targeting of ArfGAP2 depends on coatomer, the building block of the COPI coat. Using a reporter fusion approach and in vitro assays, we identified several functional elements in ArfGAP2/3. We show that the Golgi localization of ArfGAP3 depends on both a central basic stretch and a carboxy-amphipathic motif. The basic stretch interacts directly with coatomer, which we found essential for the catalytic activity of ArfGAP3 on Arf1-GTP, whereas the carboxy-amphipathic motif interacts directly with lipid membranes but has minor role in the regulation of ArfGAP3 activity. Our findings indicate that the two types of ArfGAP proteins that reside at the Golgi use a different combination of protein–protein and protein–lipid interactions to promote GTP hydrolysis in Arf1-GTP.

Bibliography

Kliouchnikov, L., Bigay, J., Mesmin, B., Parnis, A., Rawet, M., Goldfeder, N., Antonny, B., & Cassel, D. (2009). Discrete Determinants in ArfGAP2/3 Conferring Golgi Localization and Regulation by the COPI Coat. Molecular Biology of the Cell, 20(3), 859â869.

Authors 8

Lena Kliouchnikov (first)
Joëlle Bigay (additional)
Bruno Mesmin (additional)
Anna Parnis (additional)
Moran Rawet (additional)
Noga Goldfeder (additional)
Bruno Antonny (additional)
Dan Cassel (additional)

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Dates

Type	When
Created	16 years, 8 months ago (Dec. 24, 2008, 8:45 p.m.)
Deposited	6 years, 1 month ago (July 14, 2019, 11:45 a.m.)
Indexed	3 weeks, 3 days ago (July 31, 2025, 11:48 p.m.)
Issued	16 years, 6 months ago (Feb. 1, 2009)
Published	16 years, 6 months ago (Feb. 1, 2009)
Published Print	16 years, 6 months ago (Feb. 1, 2009)

Funders 0

None

BibTeX

@article{Kliouchnikov_2009, title={Discrete Determinants in ArfGAP2/3 Conferring Golgi Localization and Regulation by the COPI Coat}, volume={20}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e08-10-1010}, DOI={10.1091/mbc.e08-10-1010}, number={3}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Kliouchnikov, Lena and Bigay, Joëlle and Mesmin, Bruno and Parnis, Anna and Rawet, Moran and Goldfeder, Noga and Antonny, Bruno and Cassel, Dan}, editor={Nakano, Akihiko}, year={2009}, month=feb, pages={859–869} }

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  "abstract": "<jats:p> From yeast to mammals, two types of GTPase-activating proteins, ArfGAP1 and ArfGAP2/3, control guanosine triphosphate (GTP) hydrolysis on the small G protein ADP-ribosylation factor (Arf) 1 at the Golgi apparatus. Although functionally interchangeable, they display little similarity outside the catalytic GTPase-activating protein (GAP) domain, suggesting differential regulation. ArfGAP1 is controlled by membrane curvature through its amphipathic lipid packing sensor motifs, whereas Golgi targeting of ArfGAP2 depends on coatomer, the building block of the COPI coat. Using a reporter fusion approach and in vitro assays, we identified several functional elements in ArfGAP2/3. We show that the Golgi localization of ArfGAP3 depends on both a central basic stretch and a carboxy-amphipathic motif. The basic stretch interacts directly with coatomer, which we found essential for the catalytic activity of ArfGAP3 on Arf1-GTP, whereas the carboxy-amphipathic motif interacts directly with lipid membranes but has minor role in the regulation of ArfGAP3 activity. Our findings indicate that the two types of ArfGAP proteins that reside at the Golgi use a different combination of protein\u2013protein and protein\u2013lipid interactions to promote GTP hydrolysis in Arf1-GTP. </jats:p>",
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  "URL": "http://dx.doi.org/10.1091/mbc.e08-10-1010",
  "relation": {},
  "ISSN": [
    "1059-1524",
    "1939-4586"
  ],
  "subject": [],
  "container-title-short": "MBoC",
  "published": {
    "date-parts": [
      [
        2009,
        2
      ]
    ]
  }
}