DOI: 10.1091/mbc.e08-04-0360. A Dual Role for Actin and Microtubule Cytoskeleton in the Transport of Golgi Units from the Nurse Cells to the Oocyte Across Ring Canals

A Dual Role for Actin and Microtubule Cytoskeleton in the Transport of Golgi Units from the Nurse Cells to the Oocyte Across Ring Canals

10.1091/mbc.e08-04-0360

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Axis specification during Drosophila embryonic development requires transfer of maternal components during oogenesis from nurse cells (NCs) into the oocyte through cytoplasmic bridges. We found that the asymmetrical distribution of Golgi, between nurse cells and the oocyte, is sustained by an active transport process. We have characterized actin basket structures that asymmetrically cap the NC side of Ring canals (RCs) connecting the oocyte. Our results suggest that these actin baskets structurally support transport mechanisms of RC transit. In addition, our tracking analysis indicates that Golgi are actively transported to the oocyte rather than diffusing. We observed that RC transit is microtubule-based and mediated at least by dynein. Finally, we show that actin networks may be involved in RC crossing through a myosin II step process, as well as in dispatching Golgi units inside the oocyte subcompartments.

Authors 4

Emmanuelle Nicolas (first)
Nicolas Chenouard (additional)
Jean-Christophe Olivo-Marin (additional)
Antoine Guichet (additional)

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Dates

Type	When
Created	16 years, 9 months ago (Nov. 12, 2008, 9:10 p.m.)
Deposited	3 years, 11 months ago (Sept. 20, 2021, 3:47 p.m.)
Indexed	5 months, 1 week ago (March 19, 2025, 10:50 a.m.)
Issued	16 years, 7 months ago (Jan. 1, 2009)
Published	16 years, 7 months ago (Jan. 1, 2009)
Published Print	16 years, 7 months ago (Jan. 1, 2009)

Funders 0

None

BibTeX

@article{Nicolas_2009, title={A Dual Role for Actin and Microtubule Cytoskeleton in the Transport of Golgi Units from the Nurse Cells to the Oocyte Across Ring Canals}, volume={20}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e08-04-0360}, DOI={10.1091/mbc.e08-04-0360}, number={1}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Nicolas, Emmanuelle and Chenouard, Nicolas and Olivo-Marin, Jean-Christophe and Guichet, Antoine}, editor={Malhotra, Vivek}, year={2009}, month=jan, pages={556–568} }

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  "abstract": "<jats:p>Axis specification during Drosophila embryonic development requires transfer of maternal components during oogenesis from nurse cells (NCs) into the oocyte through cytoplasmic bridges. We found that the asymmetrical distribution of Golgi, between nurse cells and the oocyte, is sustained by an active transport process. We have characterized actin basket structures that asymmetrically cap the NC side of Ring canals (RCs) connecting the oocyte. Our results suggest that these actin baskets structurally support transport mechanisms of RC transit. In addition, our tracking analysis indicates that Golgi are actively transported to the oocyte rather than diffusing. We observed that RC transit is microtubule-based and mediated at least by dynein. Finally, we show that actin networks may be involved in RC crossing through a myosin II step process, as well as in dispatching Golgi units inside the oocyte subcompartments.</jats:p>",
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