DOI: 10.1091/mbc.e07-03-0199. Analysis of P-Body Assembly in<i>Saccharomyces cerevisiae</i>

Analysis of P-Body Assembly inSaccharomyces cerevisiae

10.1091/mbc.e07-03-0199

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Recent experiments have defined cytoplasmic foci, referred to as processing bodies (P-bodies), that contain untranslating mRNAs in conjunction with proteins involved in translation repression and mRNA decapping and degradation. However, the order of protein assembly into P-bodies and the interactions that promote P-body assembly are unknown. To gain insight into how yeast P-bodies assemble, we examined the P-body accumulation of Dcp1p, Dcp2p, Edc3p, Dhh1p, Pat1p, Lsm1p, Xrn1p, Ccr4p, and Pop2p in deletion mutants lacking one or more P-body component. These experiments revealed that Dcp2p and Pat1p are required for recruitment of Dcp1p and of the Lsm1-7p complex to P-bodies, respectively. We also demonstrate that P-body assembly is redundant and no single known component of P-bodies is required for P-body assembly, although both Dcp2p and Pat1p contribute to P-body assembly. In addition, our results indicate that Pat1p can be a nuclear-cytoplasmic shuttling protein and acts early in P-body assembly. In contrast, the Lsm1-7p complex appears to primarily function in a rate limiting step after P-body assembly in triggering decapping. Taken together, these results provide insight both into the function of individual proteins involved in mRNA degradation and the mechanisms by which yeast P-bodies assemble.

Bibliography

Teixeira, D., & Parker, R. (2007). Analysis of P-Body Assembly inSaccharomyces cerevisiae. Molecular Biology of the Cell, 18(6), 2274â2287.

Authors 2

Daniela Teixeira (first)
Roy Parker (additional)

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Dates

Type	When
Created	18 years, 4 months ago (April 11, 2007, 8:51 p.m.)
Deposited	2 years, 3 months ago (May 11, 2023, 8:11 a.m.)
Indexed	1 month, 1 week ago (July 16, 2025, 8 a.m.)
Issued	18 years, 2 months ago (June 1, 2007)
Published	18 years, 2 months ago (June 1, 2007)
Published Print	18 years, 2 months ago (June 1, 2007)

Funders 0

None

BibTeX

@article{Teixeira_2007, title={Analysis of P-Body Assembly inSaccharomyces cerevisiae}, volume={18}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e07-03-0199}, DOI={10.1091/mbc.e07-03-0199}, number={6}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Teixeira, Daniela and Parker, Roy}, editor={Fox, Thomas}, year={2007}, month=jun, pages={2274–2287} }

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