DOI: 10.1091/mbc.e06-06-0478. Initiation of Protein Synthesis by Hepatitis C Virus Is Refractory to Reduced eIF2 · GTP · Met-tRNA<sub>i</sub><sup>Met</sup>Ternary Complex Availability

Initiation of Protein Synthesis by Hepatitis C Virus Is Refractory to Reduced eIF2 · GTP · Met-tRNAiMetTernary Complex Availability

10.1091/mbc.e06-06-0478

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

A cornerstone of the antiviral interferon response is phosphorylation of eukaryotic initiation factor (eIF)2α. This limits the availability of eIF2·GTP·Met-tRNAiMetternary complexes, reduces formation of 43S preinitiation complexes, and blocks viral (and most cellular) mRNA translation. However, many viruses have developed counterstrategies that circumvent this cellular response. Herein, we characterize a novel class of translation initiation inhibitors that block ternary complex formation and prevent the assembly of 43S preinitiation complexes. We find that translation driven by the HCV IRES is refractory to inhibition by these compounds at concentrations that effectively block cap-dependent translation in vitro and in vivo. Analysis of initiation complexes formed on the HCV IRES in the presence of inhibitor indicates that eIF2α and Met-tRNAiMetare present, defining a tactic used by HCV to evade part of the antiviral interferon response.

Bibliography

Robert, F., Kapp, L. D., Khan, S. N., Acker, M. G., Kolitz, S., Kazemi, S., Kaufman, R. J., Merrick, W. C., Koromilas, A. E., Lorsch, J. R., & Pelletier, J. (2006). Initiation of Protein Synthesis by Hepatitis C Virus Is Refractory to Reduced eIF2 Â· GTP Â· Met-tRNAiMetTernary Complex Availability. Molecular Biology of the Cell, 17(11), 4632â4644.

Authors 11

Francis Robert (first)
Lee D. Kapp (additional)
Shakila N. Khan (additional)
Michael G. Acker (additional)
Sarah Kolitz (additional)
Shirin Kazemi (additional)
Randal J. Kaufman (additional)
William C. Merrick (additional)
Antonis E. Koromilas (additional)
Jon R. Lorsch (additional)
Jerry Pelletier (additional)

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Dates

Type	When
Created	19 years ago (Aug. 23, 2006, 8:59 p.m.)
Deposited	4 years ago (July 31, 2021, 12:13 p.m.)
Indexed	3 months ago (May 26, 2025, 8:27 a.m.)
Issued	18 years, 9 months ago (Nov. 1, 2006)
Published	18 years, 9 months ago (Nov. 1, 2006)
Published Print	18 years, 9 months ago (Nov. 1, 2006)

Funders 0

None

BibTeX

@article{Robert_2006, title={Initiation of Protein Synthesis by Hepatitis C Virus Is Refractory to Reduced eIF2 · GTP · Met-tRNAiMetTernary Complex Availability}, volume={17}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e06-06-0478}, DOI={10.1091/mbc.e06-06-0478}, number={11}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Robert, Francis and Kapp, Lee D. and Khan, Shakila N. and Acker, Michael G. and Kolitz, Sarah and Kazemi, Shirin and Kaufman, Randal J. and Merrick, William C. and Koromilas, Antonis E. and Lorsch, Jon R. and Pelletier, Jerry}, editor={Schmid, Sandra}, year={2006}, month=nov, pages={4632–4644} }

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