DOI: 10.1091/mbc.e06-04-0291. A Cluster of Ring Stage–specific Genes Linked to a Locus Implicated in Cytoadherence in<i>Plasmodium falciparum</i>Codes for PEXEL-negative and PEXEL-positive Proteins Exported into the Host Cell

A Cluster of Ring Stage–specific Genes Linked to a Locus Implicated in Cytoadherence inPlasmodium falciparumCodes for PEXEL-negative and PEXEL-positive Proteins Exported into the Host Cell

10.1091/mbc.e06-04-0291

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Blood stages of Plasmodium falciparum export proteins into their erythrocyte host, thereby inducing extensive host cell modifications that become apparent after the first half of the asexual development cycle (ring stage). This is responsible for a major part of parasite virulence. Export of many parasite proteins depends on a sequence motif termed Plasmodium export element (PEXEL) or vacuolar transport signal (VTS). This motif has allowed the prediction of the Plasmodium exportome. Using published genome sequence, we redetermined the boundaries of a previously studied region linked to P. falciparum virulence, reducing the number of candidate genes in this region to 13. Among these, we identified a cluster of four ring stage-specific genes, one of which is known to encode an exported protein. We demonstrate that all four genes code for proteins exported into the host cell, although only two genes contain an obvious PEXEL/VTS motif. We propose that the systematic analysis of ring stage-specific genes will reveal a cohort of exported proteins not present in the currently predicted exportome. Moreover, this provides further evidence that host cell remodeling is a major task of this developmental stage. Biochemical and photobleaching studies using these proteins reveal new properties of the parasite-induced membrane compartments in the host cell. This has important implications for the biogenesis and connectivity of these structures.

Bibliography

Spielmann, T., Hawthorne, P. L., Dixon, M. W. A., Hannemann, M., Klotz, K., Kemp, D. J., Klonis, N., Tilley, L., Trenholme, K. R., & Gardiner, D. L. (2006). A Cluster of Ring Stageâspecific Genes Linked to a Locus Implicated in Cytoadherence inPlasmodium falciparumCodes for PEXEL-negative and PEXEL-positive Proteins Exported into the Host Cell. Molecular Biology of the Cell, 17(8), 3613â3624.

Authors 10

Tobias Spielmann (first)
Paula L. Hawthorne (additional)
Matthew W.A. Dixon (additional)
Mandy Hannemann (additional)
Kathleen Klotz (additional)
David J. Kemp (additional)
Nectarios Klonis (additional)
Leann Tilley (additional)
Katharine R. Trenholme (additional)
Donald L. Gardiner (additional)

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Dates

Type	When
Created	19 years, 2 months ago (June 7, 2006, 8:59 p.m.)
Deposited	5 years, 4 months ago (April 15, 2020, 5:59 a.m.)
Indexed	1 month, 1 week ago (July 24, 2025, 8:26 a.m.)
Issued	19 years, 1 month ago (Aug. 1, 2006)
Published	19 years, 1 month ago (Aug. 1, 2006)
Published Print	19 years, 1 month ago (Aug. 1, 2006)

Funders 0

None

BibTeX

@article{Spielmann_2006, title={A Cluster of Ring Stage–specific Genes Linked to a Locus Implicated in Cytoadherence inPlasmodium falciparumCodes for PEXEL-negative and PEXEL-positive Proteins Exported into the Host Cell}, volume={17}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e06-04-0291}, DOI={10.1091/mbc.e06-04-0291}, number={8}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Spielmann, Tobias and Hawthorne, Paula L. and Dixon, Matthew W.A. and Hannemann, Mandy and Klotz, Kathleen and Kemp, David J. and Klonis, Nectarios and Tilley, Leann and Trenholme, Katharine R. and Gardiner, Donald L.}, editor={Isberg, Ralph}, year={2006}, month=aug, pages={3613–3624} }

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