DOI: 10.1091/mbc.e05-11-1002. Domains within the GARP Subunit Vps54 Confer Separate Functions in Complex Assembly and Early Endosome Recognition

Domains within the GARP Subunit Vps54 Confer Separate Functions in Complex Assembly and Early Endosome Recognition

10.1091/mbc.e05-11-1002

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Tethering complexes contribute to the specificity of membrane fusion by recognizing organelle features on both donor and acceptor membranes. The Golgi-associated retrograde protein (GARP) complex is required for retrograde traffic from both early and late endosomes to the trans-Golgi network (TGN), presenting a paradox as to how a single complex can interact specifically with vesicles from multiple upstream compartments. We have found that a subunit of the GARP complex, Vps54, can be separated into N- and C-terminal regions that have different functions. Whereas the N-terminus of Vps54 is important for GARP complex assembly and stability, a conserved C-terminal domain mediates localization to an early endocytic compartment. Mutation of this C-terminal domain has no effect on retrograde transport from late endosomes. However, a specific defect in retrieval of Snc1 from early endosomes is observed when recycling from late endosomes to the Golgi is blocked. These data suggest that separate domains recruit tethering complexes to different upstream compartments to regulate individual trafficking pathways.

Bibliography

Quenneville, N. R., Chao, T.-Y., McCaffery, J. M., & Conibear, E. (2006). Domains within the GARP Subunit Vps54 Confer Separate Functions in Complex Assembly and Early Endosome Recognition. Molecular Biology of the Cell, 17(4), 1859â1870.

Authors 4

Nicole R. Quenneville (first)
Tzu-Yuan Chao (additional)
J. Michael McCaffery (additional)
Elizabeth Conibear (additional)

References 56 Referenced 43

Dates

Type	When
Created	19 years, 6 months ago (Feb. 1, 2006, 9:09 p.m.)
Deposited	4 years, 1 month ago (July 23, 2021, 7:52 a.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 1:52 p.m.)
Issued	19 years, 4 months ago (April 1, 2006)
Published	19 years, 4 months ago (April 1, 2006)
Published Print	19 years, 4 months ago (April 1, 2006)

Funders 0

None

BibTeX

@article{Quenneville_2006, title={Domains within the GARP Subunit Vps54 Confer Separate Functions in Complex Assembly and Early Endosome Recognition}, volume={17}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e05-11-1002}, DOI={10.1091/mbc.e05-11-1002}, number={4}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Quenneville, Nicole R. and Chao, Tzu-Yuan and McCaffery, J. Michael and Conibear, Elizabeth}, year={2006}, month=apr, pages={1859–1870} }

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  "abstract": "<jats:p>Tethering complexes contribute to the specificity of membrane fusion by recognizing organelle features on both donor and acceptor membranes. The Golgi-associated retrograde protein (GARP) complex is required for retrograde traffic from both early and late endosomes to the trans-Golgi network (TGN), presenting a paradox as to how a single complex can interact specifically with vesicles from multiple upstream compartments. We have found that a subunit of the GARP complex, Vps54, can be separated into N- and C-terminal regions that have different functions. Whereas the N-terminus of Vps54 is important for GARP complex assembly and stability, a conserved C-terminal domain mediates localization to an early endocytic compartment. Mutation of this C-terminal domain has no effect on retrograde transport from late endosomes. However, a specific defect in retrieval of Snc1 from early endosomes is observed when recycling from late endosomes to the Golgi is blocked. These data suggest that separate domains recruit tethering complexes to different upstream compartments to regulate individual trafficking pathways.</jats:p>",
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