Pml39, a Novel Protein of the Nuclear Periphery Required for Nuclear Retention of Improper Messenger Ribonucleoparticles
10.1091/mbc.e05-06-0527
Crossref journal-article
American Society for Cell Biology (ASCB)
Molecular Biology of the Cell (1076)
Abstract

Using a genetic screen, we have identified a previously uncharacterized Saccharomyces cerevisiae open reading frame (renamed PML39) that displays a specific interaction with nucleoporins of the Nup84 complex. Localization of a Pml39-green fluorescent protein (GFP) fusion and two-hybrid studies revealed that Pml39 is mainly docked to a subset of nuclear pore complexes opposite to the nucleolus through interactions with Mlp1 and Mlp2. The absence of Pml39 leads to a specific leakage of unspliced mRNAs that is not enhanced upon MLP1 deletion. In addition, overexpression of PML39-GFP induces a specific trapping of mRNAs transcribed from an intron-containing reporter and of the heterogenous nuclear ribonucleoprotein Nab2 within discrete nuclear domains. In a nup60Δ mutant, Pml39 is mislocalized together with Mlp1 and Mlp2 in intranuclear foci that also recruit Nab2. Moreover, pml39Δ partially rescues the thermosensitive phenotypes of messenger ribonucleoparticles (mRNPs) assembly mutants, indicating that PML39 deletion also bypasses the requirement for normally assembled mRNPs. Together, these data indicate that Pml39 is an upstream effector of the Mlps, involved in the retention of improper mRNPs in the nucleus before their export.

Bibliography

Palancade, B., Zuccolo, M., Loeillet, S., Nicolas, A., & Doye, V. (2005). Pml39, a Novel Protein of the Nuclear Periphery Required for Nuclear Retention of Improper Messenger Ribonucleoparticles. Molecular Biology of the Cell, 16(11), 5258–5268.

Authors 5
  1. Benoît Palancade (first)
  2. Michela Zuccolo (additional)
  3. Sophie Loeillet (additional)
  4. Alain Nicolas (additional)
  5. Valérie Doye (additional)
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Dates
Type When
Created 19 years, 11 months ago (Sept. 14, 2005, 8:33 p.m.)
Deposited 6 years, 1 month ago (July 14, 2019, 10:17 a.m.)
Indexed 2 months ago (July 1, 2025, 1:26 p.m.)
Issued 19 years, 10 months ago (Nov. 1, 2005)
Published 19 years, 10 months ago (Nov. 1, 2005)
Published Print 19 years, 10 months ago (Nov. 1, 2005)
Funders 0

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@article{Palancade_2005, title={Pml39, a Novel Protein of the Nuclear Periphery Required for Nuclear Retention of Improper Messenger Ribonucleoparticles}, volume={16}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e05-06-0527}, DOI={10.1091/mbc.e05-06-0527}, number={11}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Palancade, Benoît and Zuccolo, Michela and Loeillet, Sophie and Nicolas, Alain and Doye, Valérie}, year={2005}, month=nov, pages={5258–5268} }