Functional Analysis of Human Microtubule-based Motor Proteins, the Kinesins and Dyneins, in Mitosis/Cytokinesis Using RNA Interference
10.1091/mbc.e05-02-0167
Crossref journal-article
American Society for Cell Biology (ASCB)
Molecular Biology of the Cell (1076)
Abstract

Microtubule (MT)-based motor proteins, kinesins and dyneins, play important roles in multiple cellular processes including cell division. In this study, we describe the generation and use of an Escherichia coli RNase III-prepared human kinesin/dynein esiRNA library to systematically analyze the functions of all human kinesin/dynein MT motor proteins. Our results indicate that at least 12 kinesins are involved in mitosis and cytokinesis. Eg5 (a member of the kinesin-5 family), Kif2A (a member of the kinesin-13 family), and KifC1 (a member of the kinesin-14 family) are crucial for spindle formation; KifC1, MCAK (a member of the kinesin-13 family), CENP-E (a member of the kinesin-7 family), Kif14 (a member of the kinesin-3 family), Kif18 (a member of the kinesin-8 family), and Kid (a member of the kinesin-10 family) are required for chromosome congression and alignment; Kif4A and Kif4B (members of the kinesin-4 family) have roles in anaphase spindle dynamics; and Kif4A, Kif4B, MKLP1, and MKLP2 (members of the kinesin-6 family) are essential for cytokinesis. Using immunofluorescence analysis, time-lapse microscopy, and rescue experiments, we investigate the roles of these 12 kinesins in detail.

Bibliography

Zhu, C., Zhao, J., Bibikova, M., Leverson, J. D., Bossy-Wetzel, E., Fan, J.-B., Abraham, R. T., & Jiang, W. (2005). Functional Analysis of Human Microtubule-based Motor Proteins, the Kinesins and Dyneins, in Mitosis/Cytokinesis Using RNA Interference. Molecular Biology of the Cell, 16(7), 3187–3199.

Authors 8
  1. Changjun Zhu (first)
  2. Jian Zhao (additional)
  3. Marina Bibikova (additional)
  4. Joel D. Leverson (additional)
  5. Ella Bossy-Wetzel (additional)
  6. Jian-Bing Fan (additional)
  7. Robert T. Abraham (additional)
  8. Wei Jiang (additional)
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Dates
Type When
Created 20 years, 4 months ago (April 20, 2005, 8:23 p.m.)
Deposited 4 years, 1 month ago (July 9, 2021, 8:28 a.m.)
Indexed 3 days, 11 hours ago (Aug. 26, 2025, 2:41 a.m.)
Issued 20 years, 1 month ago (July 1, 2005)
Published 20 years, 1 month ago (July 1, 2005)
Published Print 20 years, 1 month ago (July 1, 2005)
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@article{Zhu_2005, title={Functional Analysis of Human Microtubule-based Motor Proteins, the Kinesins and Dyneins, in Mitosis/Cytokinesis Using RNA Interference}, volume={16}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e05-02-0167}, DOI={10.1091/mbc.e05-02-0167}, number={7}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Zhu, Changjun and Zhao, Jian and Bibikova, Marina and Leverson, Joel D. and Bossy-Wetzel, Ella and Fan, Jian-Bing and Abraham, Robert T. and Jiang, Wei}, year={2005}, month=jul, pages={3187–3199} }