Cdc42 Is Not Essential for Filopodium Formation, Directed Migration, Cell Polarization, and Mitosis in Fibroblastoid Cells
10.1091/mbc.e05-01-0061
Crossref journal-article
American Society for Cell Biology (ASCB)
Molecular Biology of the Cell (1076)
Abstract

Cdc42 is a small GTPase involved in the regulation of the cytoskeleton and cell polarity. To test whether Cdc42 has an essential role in the formation of filopodia or directed cell migration, we generated Cdc42-deficient fibroblastoid cells by conditional gene inactivation. We report here that loss of Cdc42 did not affect filopodium or lamellipodium formation and had no significant influence on the speed of directed migration nor on mitosis. Cdc42-deficient cells displayed a more elongated cell shape and had a reduced area. Furthermore, directionality during migration and reorientation of the Golgi apparatus into the direction of migration was decreased. However, expression of dominant negative Cdc42 in Cdc42-null cells resulted in strongly reduced directed migration, severely reduced single cell directionality, and complete loss of Golgi polarization and of directionality of protrusion formation toward the wound, as well as membrane blebbing. Thus, our data show that besides Cdc42 additional GTPases of the Rho-family, which share GEFs with Cdc42, are involved in the establishment and maintenance of cell polarity during directed migration.

Bibliography

Czuchra, A., Wu, X., Meyer, H., van Hengel, J., Schroeder, T., Geffers, R., Rottner, K., & Brakebusch, C. (2005). Cdc42 Is Not Essential for Filopodium Formation, Directed Migration, Cell Polarization, and Mitosis in Fibroblastoid Cells. Molecular Biology of the Cell, 16(10), 4473–4484.

Authors 8
  1. Aleksandra Czuchra (first)
  2. Xunwei Wu (additional)
  3. Hannelore Meyer (additional)
  4. Jolanda van Hengel (additional)
  5. Timm Schroeder (additional)
  6. Robert Geffers (additional)
  7. Klemens Rottner (additional)
  8. Cord Brakebusch (additional)
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Dates
Type When
Created 20 years, 1 month ago (July 13, 2005, 4:10 a.m.)
Deposited 6 years, 1 month ago (July 14, 2019, 6:09 a.m.)
Indexed 1 month, 1 week ago (July 14, 2025, 11:25 p.m.)
Issued 19 years, 10 months ago (Oct. 1, 2005)
Published 19 years, 10 months ago (Oct. 1, 2005)
Published Print 19 years, 10 months ago (Oct. 1, 2005)
Funders 0

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@article{Czuchra_2005, title={Cdc42 Is Not Essential for Filopodium Formation, Directed Migration, Cell Polarization, and Mitosis in Fibroblastoid Cells}, volume={16}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e05-01-0061}, DOI={10.1091/mbc.e05-01-0061}, number={10}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Czuchra, Aleksandra and Wu, Xunwei and Meyer, Hannelore and van Hengel, Jolanda and Schroeder, Timm and Geffers, Robert and Rottner, Klemens and Brakebusch, Cord}, year={2005}, month=oct, pages={4473–4484} }