Arf1p Provides an Unexpected Link between COPI Vesicles and mRNA inSaccharomyces cerevisiae
10.1091/mbc.e04-05-0411
Crossref journal-article
American Society for Cell Biology (ASCB)
Molecular Biology of the Cell (1076)
Abstract

The small GTPase Arf1p is involved in different cellular processes that require its accumulation at specific cellular locations. The recruitment of Arf1p to distinct points of action might be achieved by association of Arf1p with different proteins. To identify new interactors of Arf1p, we performed an affinity chromatography with GTP- or GDP-bound Arf1p proteins. A new interactor of Arf1p-GTP was identified as Pab1p, which binds to the polyA-tail of mRNAs. Pab1p was found to associate with purified COPI-coated vesicles generated from Golgi membranes in vitro. The stability of the Pab1p–Arf1p complex depends on the presence of mRNA. Both symmetrically distributed mRNAs as well as the asymmetrically localized ASH1 mRNA are found in association with Arf1p. Remarkably, Arf1p and Pab1p are both required to restrict ASH1 mRNA to the bud tip. Arf1p and coatomer play an unexpected role in localizing mRNA independent and downstream of the SHE machinery. Hereby acts the SHE machinery in long-range mRNA transport, whereas COPI vesicles could act as short-range and localization vehicles. The endoplasmic reticulum (ER)–Golgi shuttle might be involved in concentrating mRNA at the ER.

Bibliography

Trautwein, M., Dengjel, J., Schirle, M., & Spang, A. (2004). Arf1p Provides an Unexpected Link between COPI Vesicles and mRNA inSaccharomyces cerevisiae. Molecular Biology of the Cell, 15(11), 5021–5037.

Authors 4
  1. Mark Trautwein (first)
  2. Jörn Dengjel (additional)
  3. Markus Schirle (additional)
  4. Anne Spang (additional)
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Dates
Type When
Created 20 years, 11 months ago (Sept. 8, 2004, 8:22 p.m.)
Deposited 4 years, 2 months ago (June 25, 2021, 11:43 a.m.)
Indexed 1 year, 1 month ago (July 12, 2024, 9:53 a.m.)
Issued 20 years, 9 months ago (Nov. 1, 2004)
Published 20 years, 9 months ago (Nov. 1, 2004)
Published Print 20 years, 9 months ago (Nov. 1, 2004)
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@article{Trautwein_2004, title={Arf1p Provides an Unexpected Link between COPI Vesicles and mRNA inSaccharomyces cerevisiae}, volume={15}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e04-05-0411}, DOI={10.1091/mbc.e04-05-0411}, number={11}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Trautwein, Mark and Dengjel, Jörn and Schirle, Markus and Spang, Anne}, year={2004}, month=nov, pages={5021–5037} }