DOI: 10.1091/mbc.e03-09-0691. HIV-1 Vpr Induces Defects in Mitosis, Cytokinesis, Nuclear Structure, and Centrosomes

HIV-1 Vpr Induces Defects in Mitosis, Cytokinesis, Nuclear Structure, and Centrosomes

10.1091/mbc.e03-09-0691

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Human immunodeficiency virus type 1 (HIV-1) Vpr is a 15-kDa accessory protein that contributes to several steps in the viral replication cycle and promotes virus-associated pathology. Previous studies demonstrated that Vpr inhibits G2/M cell cycle progression in both human cells and in the fission yeast Schizosaccharomyces pombe. Here, we report that, upon induction of vpr expression, fission yeast exhibited numerous defects in the assembly and function of the mitotic spindle. In particular, two spindle pole body proteins, sad1p and the polo kinase plo1p, were delocalized in vpr-expressing yeast cells, suggesting that spindle pole body integrity was perturbed. In addition, nuclear envelope structure, contractile actin ring formation, and cytokinesis were also disrupted. Similar Vpr-induced defects in mitosis and cytokinesis were observed in human cells, including aberrant mitotic spindles, multiple centrosomes, and multinucleate cells. These defects in cell division and centrosomes might account for some of the pathological effects associated with HIV-1 infection.

Bibliography

Chang, F., Re, F., Sebastian, S., Sazer, S., & Luban, J. (2004). HIV-1 Vpr Induces Defects in Mitosis, Cytokinesis, Nuclear Structure, and Centrosomes. Molecular Biology of the Cell, 15(4), 1793â1801.

Authors 5

Fred Chang (first)
Fabio Re (additional)
Sarah Sebastian (additional)
Shelley Sazer (additional)
Jeremy Luban (additional)

References 54 Referenced 45

Dates

Type	When
Created	21 years, 6 months ago (Feb. 9, 2004, 8:44 p.m.)
Deposited	4 years, 2 months ago (June 14, 2021, 1:24 p.m.)
Indexed	2 months ago (July 1, 2025, 8:33 a.m.)
Issued	21 years, 5 months ago (April 1, 2004)
Published	21 years, 5 months ago (April 1, 2004)
Published Print	21 years, 5 months ago (April 1, 2004)

Funders 0

None

BibTeX

@article{Chang_2004, title={HIV-1 Vpr Induces Defects in Mitosis, Cytokinesis, Nuclear Structure, and Centrosomes}, volume={15}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e03-09-0691}, DOI={10.1091/mbc.e03-09-0691}, number={4}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Chang, Fred and Re, Fabio and Sebastian, Sarah and Sazer, Shelley and Luban, Jeremy}, year={2004}, month=apr, pages={1793–1801} }

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  "abstract": "<jats:p>Human immunodeficiency virus type 1 (HIV-1) Vpr is a 15-kDa accessory protein that contributes to several steps in the viral replication cycle and promotes virus-associated pathology. Previous studies demonstrated that Vpr inhibits G2/M cell cycle progression in both human cells and in the fission yeast Schizosaccharomyces pombe. Here, we report that, upon induction of vpr expression, fission yeast exhibited numerous defects in the assembly and function of the mitotic spindle. In particular, two spindle pole body proteins, sad1p and the polo kinase plo1p, were delocalized in vpr-expressing yeast cells, suggesting that spindle pole body integrity was perturbed. In addition, nuclear envelope structure, contractile actin ring formation, and cytokinesis were also disrupted. Similar Vpr-induced defects in mitosis and cytokinesis were observed in human cells, including aberrant mitotic spindles, multiple centrosomes, and multinucleate cells. These defects in cell division and centrosomes might account for some of the pathological effects associated with HIV-1 infection.</jats:p>",
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