DOI: 10.1091/mbc.e03-07-0531. Novel Kelch-like Protein, KLEIP, Is Involved in Actin Assembly at Cell-Cell Contact Sites of Madin-Darby Canine Kidney Cells

Novel Kelch-like Protein, KLEIP, Is Involved in Actin Assembly at Cell-Cell Contact Sites of Madin-Darby Canine Kidney Cells

10.1091/mbc.e03-07-0531

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Dynamic rearrangements of cell-cell adhesion underlie a diverse range of physiological processes, but their precise molecular mechanisms are still obscure. Thus, identification of novel players that are involved in cell-cell adhesion would be important. We isolated a human kelch-related protein, Kelch-like ECT2 interacting protein (KLEIP), which contains the broad-complex, tramtrack, bric-a-brac (BTB)/poxvirus, zinc finger (POZ) motif and six-tandem kelch repeats. KLEIP interacted with F-actin and was concentrated at cell-cell contact sites of Madin-Darby canine kidney cells, where it colocalized with F-actin. Interestingly, this localization took place transiently during the induction of cell-cell contact and was not seen at mature junctions. KLEIP recruitment and actin assembly were induced around E-cadherin–coated beads placed on cell surfaces. The actin depolymerizing agent cytochalasin B inhibited this KLEIP recruitment around E-cadherin–coated beads. Moreover, constitutively active Rac1 enhanced the recruitment of KLEIP as well as F-actin to the adhesion sites. These observations strongly suggest that KLEIP is localized on actin filaments at the contact sites. We also found that N-terminal half of KLEIP, which lacks the actin-binding site and contains the sufficient sequence for the localization at the cell-cell contact sites, inhibited constitutively active Rac1-induced actin assembly at the contact sites. We propose that KLEIP is involved in Rac1-induced actin organization during cell-cell contact in Madin-Darby canine kidney cells.

Bibliography

Hara, T., Ishida, H., Raziuddin, R., Dorkhom, S., Kamijo, K., & Miki, T. (2004). Novel Kelch-like Protein, KLEIP, Is Involved in Actin Assembly at Cell-Cell Contact Sites of Madin-Darby Canine Kidney Cells. Molecular Biology of the Cell, 15(3), 1172â1184.

Authors 6

Takahiko Hara (first)
Hiroshi Ishida (additional)
Razi Raziuddin (additional)
Stephan Dorkhom (additional)
Keiju Kamijo (additional)
Toru Miki (additional)

References 39 Referenced 30

Dates

Type	When
Created	21 years, 8 months ago (Dec. 15, 2003, 8:13 p.m.)
Deposited	1 year, 7 months ago (Jan. 11, 2024, 12:41 p.m.)
Indexed	1 year, 3 months ago (May 12, 2024, 3:16 p.m.)
Issued	21 years, 6 months ago (March 1, 2004)
Published	21 years, 6 months ago (March 1, 2004)
Published Print	21 years, 6 months ago (March 1, 2004)

Funders 0

None

BibTeX

@article{Hara_2004, title={Novel Kelch-like Protein, KLEIP, Is Involved in Actin Assembly at Cell-Cell Contact Sites of Madin-Darby Canine Kidney Cells}, volume={15}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e03-07-0531}, DOI={10.1091/mbc.e03-07-0531}, number={3}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Hara, Takahiko and Ishida, Hiroshi and Raziuddin, Razi and Dorkhom, Stephan and Kamijo, Keiju and Miki, Toru}, year={2004}, month=mar, pages={1172–1184} }

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  "abstract": "<jats:p>Dynamic rearrangements of cell-cell adhesion underlie a diverse range of physiological processes, but their precise molecular mechanisms are still obscure. Thus, identification of novel players that are involved in cell-cell adhesion would be important. We isolated a human kelch-related protein, Kelch-like ECT2 interacting protein (KLEIP), which contains the broad-complex, tramtrack, bric-a-brac (BTB)/poxvirus, zinc finger (POZ) motif and six-tandem kelch repeats. KLEIP interacted with F-actin and was concentrated at cell-cell contact sites of Madin-Darby canine kidney cells, where it colocalized with F-actin. Interestingly, this localization took place transiently during the induction of cell-cell contact and was not seen at mature junctions. KLEIP recruitment and actin assembly were induced around E-cadherin\u2013coated beads placed on cell surfaces. The actin depolymerizing agent cytochalasin B inhibited this KLEIP recruitment around E-cadherin\u2013coated beads. Moreover, constitutively active Rac1 enhanced the recruitment of KLEIP as well as F-actin to the adhesion sites. These observations strongly suggest that KLEIP is localized on actin filaments at the contact sites. We also found that N-terminal half of KLEIP, which lacks the actin-binding site and contains the sufficient sequence for the localization at the cell-cell contact sites, inhibited constitutively active Rac1-induced actin assembly at the contact sites. We propose that KLEIP is involved in Rac1-induced actin organization during cell-cell contact in Madin-Darby canine kidney cells.</jats:p>",
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