Sti1 and Cdc37 Can Stabilize Hsp90 in Chaperone Complexes with a Protein Kinase
10.1091/mbc.e03-07-0480
Crossref journal-article
American Society for Cell Biology (ASCB)
Molecular Biology of the Cell (1076)
Abstract

Hsp90 functions in association with several cochaperones for folding of protein kinases and transcription factors, although the relative contribution of each to the overall reaction is unknown. We assayed the role of nine different cochaperones in the activation of Ste11, a Saccharomyces cerevisiae mitogen-activated protein kinase kinase kinase. Studies on signaling via this protein kinase pathway was measured by α-factor-stimulated induction of FIG1 or lacZ, and repression of HHF1. Several cochaperone mutants tested had reduced FIG1 induction or HHF1 repression, although to differing extents. The greatest defects were in cpr7Δ, sse1Δ, and ydj1Δ mutants. Assays of Ste11 kinase activity revealed a pattern of defects in the cochaperone mutant strains that were similar to the gene expression studies. Overexpression of CDC37, a chaperone required for protein kinase folding, suppressed defects the sti1Δ mutant back to wild-type levels. CDC37 overexpression also restored stable Hsp90 binding to the Ste11 protein kinase domain in the sti1Δ mutant strain. These data suggest that Cdc37 and Sti1 have functional overlap in stabilizing Hsp90:client complexes. Finally, we show that Cns1 functions in MAP kinase signaling in association with Cpr7.

Bibliography

Lee, P., Shabbir, A., Cardozo, C., & Caplan, A. J. (2004). Sti1 and Cdc37 Can Stabilize Hsp90 in Chaperone Complexes with a Protein Kinase. Molecular Biology of the Cell, 15(4), 1785–1792.

Authors 4
  1. Paul Lee (first)
  2. Arsalan Shabbir (additional)
  3. Christopher Cardozo (additional)
  4. Avrom J. Caplan (additional)
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Dates
Type When
Created 21 years, 7 months ago (Jan. 26, 2004, 8:33 p.m.)
Deposited 4 years, 2 months ago (June 13, 2021, 11:54 p.m.)
Indexed 1 year, 1 month ago (July 23, 2024, 12:32 a.m.)
Issued 21 years, 4 months ago (April 1, 2004)
Published 21 years, 4 months ago (April 1, 2004)
Published Print 21 years, 4 months ago (April 1, 2004)
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@article{Lee_2004, title={Sti1 and Cdc37 Can Stabilize Hsp90 in Chaperone Complexes with a Protein Kinase}, volume={15}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e03-07-0480}, DOI={10.1091/mbc.e03-07-0480}, number={4}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Lee, Paul and Shabbir, Arsalan and Cardozo, Christopher and Caplan, Avrom J.}, year={2004}, month=apr, pages={1785–1792} }