DOI: 10.1091/mbc.e03-04-0235. CRB3 Binds Directly to Par6 and Regulates the Morphogenesis of the Tight Junctions in Mammalian Epithelial Cells

CRB3 Binds Directly to Par6 and Regulates the Morphogenesis of the Tight Junctions in Mammalian Epithelial Cells

10.1091/mbc.e03-04-0235

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Crumbs is an apical transmembrane protein crucial for epithelial morphogenesis in Drosophila melanogaster embryos. A protein with all the characteristics for a Crumbs homologue has been identified from patients suffering from retinitis pigmentosa group 12, but this protein (CRB1) is only expressed in retina and some parts of the brain, both in human and mouse. Here, we describe CRB3, another Crumbs homologue that is preferentially expressed in epithelial tissues and skeletal muscles in human. CRB3 shares the conserved cytoplasmic domain with other Crumbs but exhibits a very short extracellular domain without the EGF- and laminin A-like G repeats present in the other Crumbs. CRB3 is localized to the apical and subapical area of epithelial cells from the mouse and human intestine, suggesting that it could play a role in epithelial morphogenesis. Indeed, expression of CRB3 or of a chimera containing the extracellular domain of the neurotrophin receptor p75NTR and the transmembrane and cytoplasmic domains of CRB3 led to a slower development of functional tight junctions in Madin-Darby canine kidney cells. This phenotype relied on the presence of CRB3 four last amino acids (ERLI) that are involved in a direct interaction with Par6, a regulator of epithelial polarity and tight junction formation. Thus, CRB3, through its cytoplasmic domain and its interactors, plays a role in apical membrane morphogenesis and tight junction regulation.

Bibliography

Lemmers, C., Michel, D., Lane-Guermonprez, L., Delgrossi, M.-H., MÃ©dina, E., Arsanto, J.-P., & Le Bivic, A. (2004). CRB3 Binds Directly to Par6 and Regulates the Morphogenesis of the Tight Junctions in Mammalian Epithelial Cells. Molecular Biology of the Cell, 15(3), 1324â1333.

Authors 7

Céline Lemmers (first)
Didier Michel (additional)
Lydie Lane-Guermonprez (additional)
Marie-Hélène Delgrossi (additional)
Emmanuelle Médina (additional)
Jean-Pierre Arsanto (additional)
André Le Bivic (additional)

References 41 Referenced 248

Dates

Type	When
Created	21 years, 7 months ago (Jan. 12, 2004, 8:24 p.m.)
Deposited	4 years, 2 months ago (June 13, 2021, 5:29 p.m.)
Indexed	1 month, 4 weeks ago (July 1, 2025, 9:47 p.m.)
Issued	21 years, 5 months ago (March 1, 2004)
Published	21 years, 5 months ago (March 1, 2004)
Published Print	21 years, 5 months ago (March 1, 2004)

Funders 0

None

BibTeX

@article{Lemmers_2004, title={CRB3 Binds Directly to Par6 and Regulates the Morphogenesis of the Tight Junctions in Mammalian Epithelial Cells}, volume={15}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e03-04-0235}, DOI={10.1091/mbc.e03-04-0235}, number={3}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Lemmers, Céline and Michel, Didier and Lane-Guermonprez, Lydie and Delgrossi, Marie-Hélène and Médina, Emmanuelle and Arsanto, Jean-Pierre and Le Bivic, André}, year={2004}, month=mar, pages={1324–1333} }

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  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2004,
        3
      ]
    ]
  },
  "references-count": 41,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          2004,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1091/mbc.e03-04-0235"
  ],
  "URL": "http://dx.doi.org/10.1091/mbc.e03-04-0235",
  "relation": {},
  "ISSN": [
    "1059-1524",
    "1939-4586"
  ],
  "subject": [],
  "container-title-short": "MBoC",
  "published": {
    "date-parts": [
      [
        2004,
        3
      ]
    ]
  }
}