DOI: 10.1091/mbc.e02-06-0338. Visualization of TGN to Endosome Trafficking through Fluorescently Labeled MPR and AP-1 in Living Cells

Visualization of TGN to Endosome Trafficking through Fluorescently Labeled MPR and AP-1 in Living Cells

10.1091/mbc.e02-06-0338

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

We have stably expressed in HeLa cells a chimeric protein made of the green fluorescent protein (GFP) fused to the transmembrane and cytoplasmic domains of the mannose 6-phosphate/insulin like growth factor II receptor in order to study its dynamics in living cells. At steady state, the bulk of this chimeric protein (GFP-CI-MPR) localizes to the trans-Golgi network (TGN), but significant amounts are also detected in peripheral, tubulo-vesicular structures and early endosomes as well as at the plasma membrane. Time-lapse videomicroscopy shows that the GFP-CI-MPR is ubiquitously detected in tubular elements that detach from the TGN and move toward the cell periphery, sometimes breaking into smaller tubular fragments. The formation of the TGN-derived tubules is temperature dependent, requires the presence of intact microtubule and actin networks, and is regulated by the ARF-1 GTPase. The TGN-derived tubules fuse with peripheral, tubulo-vesicular structures also containing the GFP-CI-MPR. These structures are highly dynamic, fusing with each other as well as with early endosomes. Time-lapse videomicroscopy performed on HeLa cells coexpressing the CFP-CI-MPR and the AP-1 complex whose γ-subunit was fused to YFP shows that AP-1 is present not only on the TGN and peripheral CFP-CI-MPR containing structures but also on TGN-derived tubules containing the CFP-CI-MPR. The data support the notion that tubular elements can mediate MPR transport from the TGN to a peripheral, tubulo-vesicular network dynamically connected with the endocytic pathway and that the AP-1 coat may facilitate MPR sorting in the TGN and endosomes.

Bibliography

Waguri, S., Dewitte, F., Le Borgne, R., RouillÃ©, Y., Uchiyama, Y., Dubremetz, J.-F., & Hoflack, B. (2003). Visualization of TGN to Endosome Trafficking through Fluorescently Labeled MPR and AP-1 in Living Cells. Molecular Biology of the Cell, 14(1), 142â155.

Authors 7

Satoshi Waguri (first)
Frédérique Dewitte (additional)
Roland Le Borgne (additional)
Yves Rouillé (additional)
Yasuo Uchiyama (additional)
Jean-François Dubremetz (additional)
Bernard Hoflack (additional)

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Dates

Type	When
Created	22 years, 7 months ago (Jan. 15, 2003, 7:23 p.m.)
Deposited	4 years, 2 months ago (June 3, 2021, 2:32 a.m.)
Indexed	3 months ago (May 24, 2025, 8:22 a.m.)
Issued	22 years, 7 months ago (Jan. 1, 2003)
Published	22 years, 7 months ago (Jan. 1, 2003)
Published Print	22 years, 7 months ago (Jan. 1, 2003)

Funders 0

None

BibTeX

@article{Waguri_2003, title={Visualization of TGN to Endosome Trafficking through Fluorescently Labeled MPR and AP-1 in Living Cells}, volume={14}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e02-06-0338}, DOI={10.1091/mbc.e02-06-0338}, number={1}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Waguri, Satoshi and Dewitte, Frédérique and Le Borgne, Roland and Rouillé, Yves and Uchiyama, Yasuo and Dubremetz, Jean-François and Hoflack, Bernard}, editor={Pfeffer, Suzanne R.}, year={2003}, month=jan, pages={142–155} }

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