DOI: 10.1091/mbc.e02-04-0205. ε-Tubulin Is an Essential Component of the Centriole

ε-Tubulin Is an Essential Component of the Centriole

10.1091/mbc.e02-04-0205

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Centrioles and basal bodies are cylinders composed of nine triplet microtubule blades that play essential roles in the centrosome and in flagellar assembly. Chlamydomonas cells with thebld2-1 mutation fail to assemble doublet and triplet microtubules and have defects in cleavage furrow placement and meiosis. Using positional cloning, we have walked 720 kb and identified a 13.2-kb fragment that contains ε-tubulin and rescues the Bld2 defects. The bld2-1 allele has a premature stop codon and intragenic revertants replace the stop codon with glutamine, glutamate, or lysine. Polyclonal antibodies to ε-tubulin show peripheral labeling of full-length basal bodies and centrioles. Thus, ε-tubulin is encoded by the BLD2 allele and ε-tubulin plays a role in basal body/centriole morphogenesis.

Bibliography

Dutcher, S. K., Morrissette, N. S., Preble, A. M., Rackley, C., & Stanga, J. (2002). Îµ-Tubulin Is an Essential Component of the Centriole. Molecular Biology of the Cell, 13(11), 3859â3869.

Authors 5

Susan K. Dutcher (first)
Naomi S. Morrissette (additional)
Andrea M. Preble (additional)
Craig Rackley (additional)
John Stanga (additional)

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Dates

Type	When
Created	22 years, 9 months ago (Nov. 12, 2002, 2:42 p.m.)
Deposited	4 years, 3 months ago (May 30, 2021, 4:19 p.m.)
Indexed	4 weeks, 2 days ago (Aug. 2, 2025, 1:07 a.m.)
Issued	22 years, 10 months ago (Nov. 1, 2002)
Published	22 years, 10 months ago (Nov. 1, 2002)
Published Print	22 years, 10 months ago (Nov. 1, 2002)

Funders 0

None

BibTeX

@article{Dutcher_2002, title={ε-Tubulin Is an Essential Component of the Centriole}, volume={13}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e02-04-0205}, DOI={10.1091/mbc.e02-04-0205}, number={11}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Dutcher, Susan K. and Morrissette, Naomi S. and Preble, Andrea M. and Rackley, Craig and Stanga, John}, editor={Salmon, Ted}, year={2002}, month=nov, pages={3859–3869} }

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  "abstract": "<jats:p>Centrioles and basal bodies are cylinders composed of nine triplet microtubule blades that play essential roles in the centrosome and in flagellar assembly. Chlamydomonas cells with thebld2-1 mutation fail to assemble doublet and triplet microtubules and have defects in cleavage furrow placement and meiosis. Using positional cloning, we have walked 720 kb and identified a 13.2-kb fragment that contains \u03b5-tubulin and rescues the Bld2 defects. The bld2-1 allele has a premature stop codon and intragenic revertants replace the stop codon with glutamine, glutamate, or lysine. Polyclonal antibodies to \u03b5-tubulin show peripheral labeling of full-length basal bodies and centrioles. Thus, \u03b5-tubulin is encoded by the BLD2 allele and \u03b5-tubulin plays a role in basal body/centriole morphogenesis.</jats:p>",
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