DOI: 10.1091/mbc.e02-03-0155. The Microtubule Plus-End Proteins EB1 and Dynactin Have Differential Effects on Microtubule Polymerization [Penn]

The Microtubule Plus-End Proteins EB1 and Dynactin Have Differential Effects on Microtubule Polymerization

10.1091/mbc.e02-03-0155

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Several microtubule-binding proteins including EB1, dynactin, APC, and CLIP-170 localize to the plus-ends of growing microtubules. Although these proteins can bind to microtubules independently, evidence for interactions among them has led to the hypothesis of a plus-end complex. Here we clarify the interaction between EB1 and dynactin and show that EB1 binds directly to the N-terminus of the p150Gluedsubunit. One function of a plus-end complex may be to regulate microtubule dynamics. Overexpression of either EB1 or p150Gluedin cultured cells bundles microtubules, suggesting that each may enhance microtubule stability. The morphology of these bundles, however, differs dramatically, indicating that EB1 and dynactin may act in different ways. Disruption of the dynactin complex augments the bundling effect of EB1, suggesting that dynactin may regulate the effect of EB1 on microtubules. In vitro assays were performed to elucidate the effects of EB1 and p150Gluedon microtubule polymerization, and they show that p150Gluedhas a potent microtubule nucleation effect, whereas EB1 has a potent elongation effect. Overall microtubule dynamics may result from a balance between the individual effects of plus-end proteins. Differences in the expression and regulation of plus-end proteins in different cell types may underlie previously noted differences in microtubule dynamics.

Bibliography

Ligon, L. A., Shelly, S. S., Tokito, M., & Holzbaur, E. L. F. (2003). The Microtubule Plus-End Proteins EB1 and Dynactin Have Differential Effects on Microtubule Polymerization. Molecular Biology of the Cell, 14(4), 1405â1417.

Authors 4

Lee A. Ligon (first)
Spencer S. Shelly (additional)
Mariko Tokito (additional)
Erika L.F. Holzbaur (additional)

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Dates

Type	When
Created	22 years, 4 months ago (April 9, 2003, 2:54 p.m.)
Deposited	4 years, 2 months ago (June 6, 2021, 1:54 a.m.)
Indexed	1 year ago (Aug. 3, 2024, 10:20 p.m.)
Issued	22 years, 4 months ago (April 1, 2003)
Published	22 years, 4 months ago (April 1, 2003)
Published Print	22 years, 4 months ago (April 1, 2003)

Funders 0

None

BibTeX

@article{Ligon_2003, title={The Microtubule Plus-End Proteins EB1 and Dynactin Have Differential Effects on Microtubule Polymerization}, volume={14}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e02-03-0155}, DOI={10.1091/mbc.e02-03-0155}, number={4}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Ligon, Lee A. and Shelly, Spencer S. and Tokito, Mariko and Holzbaur, Erika L.F.}, editor={Stearns, Tim}, year={2003}, month=apr, pages={1405–1417} }

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  "abstract": "<jats:p>Several microtubule-binding proteins including EB1, dynactin, APC, and CLIP-170 localize to the plus-ends of growing microtubules. Although these proteins can bind to microtubules independently, evidence for interactions among them has led to the hypothesis of a plus-end complex. Here we clarify the interaction between EB1 and dynactin and show that EB1 binds directly to the N-terminus of the p150<jats:sup>Glued</jats:sup>subunit. One function of a plus-end complex may be to regulate microtubule dynamics. Overexpression of either EB1 or p150<jats:sup>Glued</jats:sup>in cultured cells bundles microtubules, suggesting that each may enhance microtubule stability. The morphology of these bundles, however, differs dramatically, indicating that EB1 and dynactin may act in different ways. Disruption of the dynactin complex augments the bundling effect of EB1, suggesting that dynactin may regulate the effect of EB1 on microtubules. In vitro assays were performed to elucidate the effects of EB1 and p150<jats:sup>Glued</jats:sup>on microtubule polymerization, and they show that p150<jats:sup>Glued</jats:sup>has a potent microtubule nucleation effect, whereas EB1 has a potent elongation effect. Overall microtubule dynamics may result from a balance between the individual effects of plus-end proteins. Differences in the expression and regulation of plus-end proteins in different cell types may underlie previously noted differences in microtubule dynamics.</jats:p>",
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