DOI: 10.1091/mbc.e02-02-0102. A Conserved <i>Drosophila</i>Transportin-Serine/Arginine-rich (SR) Protein Permits Nuclear Import of<i>Drosophila</i> SR Protein Splicing Factors and Their Antagonist Repressor Splicing Factor 1

A Conserved DrosophilaTransportin-Serine/Arginine-rich (SR) Protein Permits Nuclear Import ofDrosophila SR Protein Splicing Factors and Their Antagonist Repressor Splicing Factor 1

10.1091/mbc.e02-02-0102

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Members of the highly conserved serine/arginine-rich (SR) protein family are nuclear factors involved in splicing of metazoan mRNA precursors. In mammals, two nuclear import receptors, transportin (TRN)-SR1 and TRN-SR2, are responsible for targeting SR proteins to the nucleus. Distinctive features in the nuclear localization signal between Drosophila and mammalian SR proteins prompted us to examine the mechanism by whichDrosophila SR proteins and their antagonist repressor splicing factor 1 (RSF1) are imported into nucleus. Herein, we report the identification and characterization of a Drosophilaimportin β-family protein (dTRN-SR), homologous to TRN-SR2, that specifically interacts with both SR proteins and RSF1. dTRN-SR has a broad localization in the cytoplasm and the nucleus, whereas an N-terminal deletion mutant colocalizes with SR proteins in nuclear speckles. Far Western experiments established that the RS domain of SR proteins and the GRS domain of RSF1 are required for the direct interaction with dTRN-SR, an interaction that can be modulated by phosphorylation. Using the yeast model system in which nuclear import of Drosophila SR proteins and RSF1 is impaired, we demonstrate that complementation with dTRN-SR is sufficient to target these proteins to the nucleus. Together, the results imply that the mechanism by which SR proteins are imported to the nucleus is conserved between Drosophila and humans.

Bibliography

Allemand, E., Dokudovskaya, S., BordonnÃ©, R., & Tazi, J. (2002). A Conserved DrosophilaTransportin-Serine/Arginine-rich (SR) Protein Permits Nuclear Import ofDrosophila SR Protein Splicing Factors and Their Antagonist Repressor Splicing Factor 1. Molecular Biology of the Cell, 13(7), 2436â2447.

Authors 4

Eric Allemand (first)
Svetlana Dokudovskaya (additional)
Rémy Bordonné (additional)
Jamal Tazi (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 28, 2002, 2:37 p.m.)
Deposited	6 years, 1 month ago (July 14, 2019, 4:38 a.m.)
Indexed	3 weeks, 3 days ago (Aug. 5, 2025, 9:03 a.m.)
Issued	23 years, 1 month ago (July 1, 2002)
Published	23 years, 1 month ago (July 1, 2002)
Published Print	23 years, 1 month ago (July 1, 2002)

Funders 0

None

BibTeX

@article{Allemand_2002, title={A Conserved DrosophilaTransportin-Serine/Arginine-rich (SR) Protein Permits Nuclear Import ofDrosophila SR Protein Splicing Factors and Their Antagonist Repressor Splicing Factor 1}, volume={13}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e02-02-0102}, DOI={10.1091/mbc.e02-02-0102}, number={7}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Allemand, Eric and Dokudovskaya, Svetlana and Bordonné, Rémy and Tazi, Jamal}, editor={Koshland, Douglas}, year={2002}, month=jul, pages={2436–2447} }

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