DOI: 10.1091/mbc.e16-03-0196. Distinct isoform-specific complexes of TANGO1 cooperatively facilitate collagen secretion from the endoplasmic reticulum

Distinct isoform-specific complexes of TANGO1 cooperatively facilitate collagen secretion from the endoplasmic reticulum

10.1091/mbc.e16-03-0196

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Collagens synthesized within the endoplasmic reticulum (ER) are too large to fit in conventional COPII-coated transport vesicles; thus their export from the ER requires specialized factors. TANGO1 (L) is an integral membrane protein that binds to collagen and the coatomer of vesicles and is necessary for collagen secretion from the ER. Here we characterized the short isoform of TANGO1 (TANGO1S), lacking the collagen-binding domain, and found that it was independently required for collagen export from the ER. Moreover, we found that each of the TANGO1 isoforms forms a stable protein complex with factors involved in collagen secretion: TANGO1L/cTAGE5/Sec12 (900 kDa) and TANGO1S/cTAGE5/Sec12 (700 kDa). Of interest, TANGO1S and TANGO1L seemed to be interchangeable in exporting collagen from the ER. Our results suggest that mammalian ER exit sites possess two different-sized membrane-bound macromolecular complexes that specifically function in large-cargo export from the ER.

Bibliography

Maeda, M., Saito, K., & Katada, T. (2016). Distinct isoform-specific complexes of TANGO1 cooperatively facilitate collagen secretion from the endoplasmic reticulum. Molecular Biology of the Cell, 27(17), 2688â2696.

Authors 3

Miharu Maeda (first)
Kota Saito (additional)
Toshiaki Katada (additional)

References 20 Referenced 56

10.1042/BJ20110554 / Biochem J by Cruz-Garcia D (2012)
10.1038/nature10822 / Nature by Jin L (2012)
10.1146/annurev-cellbio-100913-013002 / Annu Rev Cell Dev Biol by Malhotra V (2015)
10.1042/BST20140286 / Biochem Soc Trans by Malhotra V (2015)
10.1016/j.celrep.2016.04.062 / Cell Rep by McCaughey J (2016)
10.1083/jcb.131.6.1715 / J Cell Biol by Nakamura N (1995)
10.7554/eLife.02784 / eLife by Nogueira C (2014)
10.1016/j.cell.2008.12.025 / Cell by Saito K (2009)
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10.1083/jcb.201312062 / J Cell Biol by Saito K (2014)
10.1038/nmeth.3047 / Nat Methods by Sanjana NE (2014)
10.1083/jcb.201603072 / J Cell Biol by Santos AJ (2016)
10.7554/eLife.10982 / Elife by Santos AJ (2015)
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10.1126/stke.3452006pl4 / Sci STKE by Swamy M (2006)
10.1091/mbc.e16-03-0180
10.1126/science.1224947 / Science by Venditti R (2012)
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10.1083/jcb.201007162 / J Cell Biol by Wilson DG (2011)

Dates

Type	When
Created	9 years, 1 month ago (July 14, 2016, 2:52 a.m.)
Deposited	3 years, 1 month ago (July 2, 2022, 10:26 p.m.)
Indexed	1 week, 3 days ago (Aug. 23, 2025, 1:13 a.m.)
Issued	9 years ago (Sept. 1, 2016)
Published	9 years ago (Sept. 1, 2016)
Published Print	9 years ago (Sept. 1, 2016)

Funders 0

None

BibTeX

@article{Maeda_2016, title={Distinct isoform-specific complexes of TANGO1 cooperatively facilitate collagen secretion from the endoplasmic reticulum}, volume={27}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e16-03-0196}, DOI={10.1091/mbc.e16-03-0196}, number={17}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Maeda, Miharu and Saito, Kota and Katada, Toshiaki}, editor={Nakano, Akihiko}, year={2016}, month=sep, pages={2688–2696} }

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