DOI: 10.1091/mbc.e11-05-0415. RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor

10.1091/mbc.e11-05-0415

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

The evolutionarily conserved Pat1 proteins are P-body components recently shown to play important roles in cytoplasmic gene expression control. Using human cell lines, we demonstrate that human Pat1b is a shuttling protein whose nuclear export is mediated via a consensus NES sequence and Crm1, as evidenced by leptomycin B (LMB) treatment. However, not all P-body components are nucleocytoplasmic proteins; rck/p54, Dcp1a, Edc3, Ge-1, and Xrn1 are insensitive to LMB and remain cytoplasmic in its presence. Nuclear Pat1b localizes to PML–associated foci and SC35-containing splicing speckles in a transcription-dependent manner, whereas in the absence of RNA synthesis, Pat1b redistributes to crescent-shaped nucleolar caps. Furthermore, inhibition of splicing by spliceostatin A leads to the reorganization of SC35 speckles, which is closely mirrored by Pat1b, indicating that it may also be involved in splicing processes. Of interest, Pat1b retention in these three nuclear compartments is mediated via distinct regions of the protein. Examination of the nuclear distribution of 4E-T(ransporter), an additional P-body nucleocytoplasmic protein, revealed that 4E-T colocalizes with Pat1b in PML-associated foci but not in nucleolar caps. Taken together, our findings strongly suggest that Pat1b participates in several RNA-related nuclear processes in addition to its multiple regulatory roles in the cytoplasm.

Bibliography

Marnef, A., Weil, D., & Standart, N. (2012). RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor. Molecular Biology of the Cell, 23(1), 213â224.

Authors 3

Aline Marnef (first)
Dominique Weil (additional)
Nancy Standart (additional)

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Dates

Type	When
Created	13 years, 9 months ago (Nov. 17, 2011, 7:32 a.m.)
Deposited	1 year, 4 months ago (April 14, 2024, 4:31 p.m.)
Indexed	1 month ago (July 30, 2025, 8:29 p.m.)
Issued	13 years, 7 months ago (Jan. 1, 2012)
Published	13 years, 7 months ago (Jan. 1, 2012)
Published Print	13 years, 7 months ago (Jan. 1, 2012)

Funders 0

None

BibTeX

@article{Marnef_2012, title={RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor}, volume={23}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e11-05-0415}, DOI={10.1091/mbc.e11-05-0415}, number={1}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Marnef, Aline and Weil, Dominique and Standart, Nancy}, editor={Matera, A. Gregory}, year={2012}, month=jan, pages={213–224} }

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  "abstract": "<jats:p>The evolutionarily conserved Pat1 proteins are P-body components recently shown to play important roles in cytoplasmic gene expression control. Using human cell lines, we demonstrate that human Pat1b is a shuttling protein whose nuclear export is mediated via a consensus NES sequence and Crm1, as evidenced by leptomycin B (LMB) treatment. However, not all P-body components are nucleocytoplasmic proteins; rck/p54, Dcp1a, Edc3, Ge-1, and Xrn1 are insensitive to LMB and remain cytoplasmic in its presence. Nuclear Pat1b localizes to PML\u2013associated foci and SC35-containing splicing speckles in a transcription-dependent manner, whereas in the absence of RNA synthesis, Pat1b redistributes to crescent-shaped nucleolar caps. Furthermore, inhibition of splicing by spliceostatin A leads to the reorganization of SC35 speckles, which is closely mirrored by Pat1b, indicating that it may also be involved in splicing processes. Of interest, Pat1b retention in these three nuclear compartments is mediated via distinct regions of the protein. Examination of the nuclear distribution of 4E-T(ransporter), an additional P-body nucleocytoplasmic protein, revealed that 4E-T colocalizes with Pat1b in PML-associated foci but not in nucleolar caps. Taken together, our findings strongly suggest that Pat1b participates in several RNA-related nuclear processes in addition to its multiple regulatory roles in the cytoplasm.</jats:p>",
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