DOI: 10.1091/mbc.e11-03-0274. Numb controls E-cadherin endocytosis through p120 catenin with aPKC

Numb controls E-cadherin endocytosis through p120 catenin with aPKC

10.1091/mbc.e11-03-0274

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Cadherin trafficking controls tissue morphogenesis and cell polarity. The endocytic adaptor Numb participates in apicobasal polarity by acting on intercellular adhesions in epithelial cells. However, it remains largely unknown how Numb controls cadherin-based adhesion. Here, we found that Numb directly interacted with p120 catenin (p120), which is known to interact with E-cadherin and prevent its internalization. Numb accumulated at intercellular adhesion sites and the apical membrane in epithelial cells. Depletion of Numb impaired E-cadherin internalization, whereas depletion of p120 accelerated internalization. Expression of the Numb-binding fragment of p120 inhibited E-cadherin internalization in a dominant-negative fashion, indicating that Numb interacts with the E-cadherin/p120 complex and promotes E-cadherin endocytosis. Impairment of Numb induced mislocalization of E-cadherin from the lateral membrane to the apical membrane. Atypical protein kinase C (aPKC), a member of the PAR complex, phosphorylated Numb and inhibited its association with p120 and α-adaptin. Depletion or inhibition of aPKC accelerated E-cadherin internalization. Wild-type Numb restored E-cadherin internalization in the Numb-depleted cells, whereas a phosphomimetic mutant or a mutant with defective α-adaptin-binding ability did not restore the internalization. Thus, we propose that aPKC phosphorylates Numb to prevent its binding to p120 and α-adaptin, thereby attenuating E-cadherin endocytosis to maintain apicobasal polarity.

Bibliography

Sato, K., Watanabe, T., Wang, S., Kakeno, M., Matsuzawa, K., Matsui, T., Yokoi, K., Murase, K., Sugiyama, I., Ozawa, M., & Kaibuchi, K. (2011). Numb controls E-cadherin endocytosis through p120 catenin with aPKC. Molecular Biology of the Cell, 22(17), 3103â3119.

Authors 11

Kazuhide Sato (first)
Takashi Watanabe (additional)
Shujie Wang (additional)
Mai Kakeno (additional)
Kenji Matsuzawa (additional)
Toshinori Matsui (additional)
Keiko Yokoi (additional)
Kiyoko Murase (additional)
Ikuko Sugiyama (additional)
Masayuki Ozawa (additional)
Kozo Kaibuchi (additional)

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Dates

Type	When
Created	14 years, 1 month ago (July 20, 2011, 7:09 p.m.)
Deposited	6 years, 1 month ago (July 14, 2019, 6:57 a.m.)
Indexed	1 month ago (July 27, 2025, 3:45 a.m.)
Issued	13 years, 11 months ago (Sept. 1, 2011)
Published	13 years, 11 months ago (Sept. 1, 2011)
Published Print	13 years, 11 months ago (Sept. 1, 2011)

Funders 0

None

BibTeX

@article{Sato_2011, title={Numb controls E-cadherin endocytosis through p120 catenin with aPKC}, volume={22}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e11-03-0274}, DOI={10.1091/mbc.e11-03-0274}, number={17}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Sato, Kazuhide and Watanabe, Takashi and Wang, Shujie and Kakeno, Mai and Matsuzawa, Kenji and Matsui, Toshinori and Yokoi, Keiko and Murase, Kiyoko and Sugiyama, Ikuko and Ozawa, Masayuki and Kaibuchi, Kozo}, editor={Yoshimori, Tamotsu}, year={2011}, month=sep, pages={3103–3119} }

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