DOI: 10.1091/mbc.e10-08-0690. Highly Ordered Spatial Organization of the Structural Long Noncoding NEAT1 RNAs within Paraspeckle Nuclear Bodies

Highly Ordered Spatial Organization of the Structural Long Noncoding NEAT1 RNAs within Paraspeckle Nuclear Bodies

10.1091/mbc.e10-08-0690

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Paraspeckles (PSPs) are nuclear bodies associated with the retention in the nucleus of specific mRNAs. Two isoforms of a long noncoding RNA (NEAT1_v1/Menε and NEAT1_v2/Menβ) are required for the integrity of PSPs. Here, we analyzed the molecular organization of PSPs by immuno- and in situ hybridization electron microscopy. Detection of the paraspeckle markers PSPC1 and P54NRB/NONO confirm the identity between PSPs and the previously described interchromatin granule-associated zones (IGAZs). High-resolution in situ hybridization of NEAT1 transcripts revealed a highly ordered organization of IGAZ/PSPs. Although the 3.7-kb NEAT1_v1 and the identical 5′ end of the 22.7-kb NEAT1_v2 transcripts are confined to the periphery, central sequences of NEAT1_v2 are found within the electron-dense core of the bodies. Moreover, the 3′ end of NEAT1_v2 also localize to the periphery, indicating possible architectures for IGAZ/PSPs. These results further suggest that the organization of NEAT1 transcripts constrains the geometry of these bodies. Accordingly, we observed in HeLa and NIH 3T3 cells that IGAZ/PSPs are elongated structures with a well-defined diameter. Our results provide new insight on the ability of noncoding RNAs to form subcellular structures.

Bibliography

Souquere, S., Beauclair, G., Harper, F., Fox, A., & Pierron, G. (2010). Highly Ordered Spatial Organization of the Structural Long Noncoding NEAT1 RNAs within Paraspeckle Nuclear Bodies. Molecular Biology of the Cell, 21(22), 4020â4027.

Authors 5

Sylvie Souquere (first)
Guillaume Beauclair (additional)
Francis Harper (additional)
Archa Fox (additional)
Gérard Pierron (additional)

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Dates

Type	When
Created	14 years, 10 months ago (Sept. 29, 2010, 6:48 p.m.)
Deposited	6 years, 1 month ago (July 14, 2019, 6:01 a.m.)
Indexed	1 day, 15 hours ago (Aug. 27, 2025, 11:45 a.m.)
Issued	14 years, 9 months ago (Nov. 15, 2010)
Published	14 years, 9 months ago (Nov. 15, 2010)
Published Print	14 years, 9 months ago (Nov. 15, 2010)

Funders 0

None

BibTeX

@article{Souquere_2010, title={Highly Ordered Spatial Organization of the Structural Long Noncoding NEAT1 RNAs within Paraspeckle Nuclear Bodies}, volume={21}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e10-08-0690}, DOI={10.1091/mbc.e10-08-0690}, number={22}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Souquere, Sylvie and Beauclair, Guillaume and Harper, Francis and Fox, Archa and Pierron, Gérard}, editor={Matera, A. Gregory}, year={2010}, month=nov, pages={4020–4027} }

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