DOI: 10.1091/mbc.e09-09-0777. Small Cajal Body–specific RNAs of<i>Drosophila</i>Function in the Absence of Cajal Bodies

Small Cajal Body–specific RNAs ofDrosophilaFunction in the Absence of Cajal Bodies

10.1091/mbc.e09-09-0777

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

During their biogenesis small nuclear RNAs (snRNAs) undergo multiple covalent modifications that require guide RNAs to direct methylase and pseudouridylase enzymes to the appropriate nucleotides. Because of their localization in the nuclear Cajal body (CB), these guide RNAs are known as small CB-specific RNAs (scaRNAs). Using a fluorescent primer extension technique, we mapped the modified nucleotides in Drosophila U1, U2, U4, and U5 snRNAs. By fluorescent in situ hybridization (FISH) we showed that seven Drosophila scaRNAs are concentrated in easily detectable CBs. We used two assays based on Xenopus oocyte nuclei to demonstrate that three of these Drosophila scaRNAs do, in fact, function as guide RNAs. In flies null for the CB marker protein coilin, CBs are absent and there are no localized FISH signals for the scaRNAs. Nevertheless, biochemical experiments show that scaRNAs are present at normal levels and snRNAs are properly modified. Our experiments demonstrate that several scaRNAs are concentrated as expected in the CBs of wild-type Drosophila, but they function equally well in the nucleoplasm of mutant flies that lack CBs. We propose that the snRNA modification machinery is not limited to CBs, but is dispersed throughout the nucleoplasm of cells in general.

Bibliography

Deryusheva, S., & Gall, J. G. (2009). Small Cajal Bodyâspecific RNAs ofDrosophilaFunction in the Absence of Cajal Bodies. Molecular Biology of the Cell, 20(24), 5250â5259.

Authors 2

Svetlana Deryusheva (first)
Joseph G. Gall (additional)

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Dates

Type	When
Created	15 years, 10 months ago (Oct. 21, 2009, 9:45 p.m.)
Deposited	2 years, 2 months ago (May 27, 2023, 3:53 a.m.)
Indexed	2 weeks, 4 days ago (Aug. 6, 2025, 7:59 a.m.)
Issued	15 years, 8 months ago (Dec. 15, 2009)
Published	15 years, 8 months ago (Dec. 15, 2009)
Published Print	15 years, 8 months ago (Dec. 15, 2009)

Funders 0

None

BibTeX

@article{Deryusheva_2009, title={Small Cajal Body–specific RNAs ofDrosophilaFunction in the Absence of Cajal Bodies}, volume={20}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e09-09-0777}, DOI={10.1091/mbc.e09-09-0777}, number={24}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Deryusheva, Svetlana and Gall, Joseph G.}, editor={Matera, A. Gregory}, year={2009}, month=dec, pages={5250–5259} }

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  "abstract": "<jats:p>During their biogenesis small nuclear RNAs (snRNAs) undergo multiple covalent modifications that require guide RNAs to direct methylase and pseudouridylase enzymes to the appropriate nucleotides. Because of their localization in the nuclear Cajal body (CB), these guide RNAs are known as small CB-specific RNAs (scaRNAs). Using a fluorescent primer extension technique, we mapped the modified nucleotides in Drosophila U1, U2, U4, and U5 snRNAs. By fluorescent in situ hybridization (FISH) we showed that seven Drosophila scaRNAs are concentrated in easily detectable CBs. We used two assays based on Xenopus oocyte nuclei to demonstrate that three of these Drosophila scaRNAs do, in fact, function as guide RNAs. In flies null for the CB marker protein coilin, CBs are absent and there are no localized FISH signals for the scaRNAs. Nevertheless, biochemical experiments show that scaRNAs are present at normal levels and snRNAs are properly modified. Our experiments demonstrate that several scaRNAs are concentrated as expected in the CBs of wild-type Drosophila, but they function equally well in the nucleoplasm of mutant flies that lack CBs. We propose that the snRNA modification machinery is not limited to CBs, but is dispersed throughout the nucleoplasm of cells in general.</jats:p>",
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