DOI: 10.1091/mbc.e09-02-0126. Son Is Essential for Nuclear Speckle Organization and Cell Cycle Progression

Son Is Essential for Nuclear Speckle Organization and Cell Cycle Progression

10.1091/mbc.e09-02-0126

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Subnuclear organization and spatiotemporal regulation of pre-mRNA processing factors is essential for the production of mature protein-coding mRNAs. We have discovered that a large protein called Son has a novel role in maintaining proper nuclear organization of pre-mRNA processing factors in nuclear speckles. The primary sequence of Son contains a concentrated region of multiple unique tandem repeat motifs that may support a role for Son as a scaffolding protein for RNA processing factors in nuclear speckles. We used RNA interference (RNAi) approaches and high-resolution microscopy techniques to study the functions of Son in the context of intact cells. Although Son precisely colocalizes with pre-mRNA splicing factors in nuclear speckles, its depletion by RNAi leads to cell cycle arrest in metaphase and causes dramatic disorganization of small nuclear ribonuclear protein and serine-arginine rich protein splicing factors during interphase. Here, we propose that Son is essential for appropriate subnuclear organization of pre-mRNA splicing factors and for promoting normal cell cycle progression.

Bibliography

Sharma, A., Takata, H., Shibahara, K., Bubulya, A., & Bubulya, P. A. (2010). Son Is Essential for Nuclear Speckle Organization and Cell Cycle Progression. Molecular Biology of the Cell, 21(4), 650â663.

Authors 5

Alok Sharma (first)
Hideaki Takata (additional)
Kei-ichi Shibahara (additional)
Athanasios Bubulya (additional)
Paula A. Bubulya (additional)

References 48 Referenced 114

10.1073/pnas.0802696105 / Proc. Natl. Acad. Sci. USA by Ahn E.-H. (2008)
10.1242/jcs.009225 / J. Cell Sci by Baguet A. (2007)
10.1016/j.ceb.2005.04.006 / Curr. Opin. Cell Biol by Bentley D. (2005)
10.1083/jcb.200404120 / J. Cell Biol by Bubulya P. A. (2004)
10.1083/jcb.138.2.225 / J. Cell Biol by Caceres J. F. (1997)
10.1128/MCB.25.8.2969-2980.2005 / Mol. Cell. Biol by Cazalla D. (2005)
10.1146/annurev.cellbio.20.010403.103738 / Annu. Rev. Cell Dev. Biol by Cioce M. (2005)
10.1128/MCB.02235-06 / Mol. Cell. Biol by Cline E. G. (2007)
10.1002/j.1460-2075.1996.tb00357.x / EMBO J by Colwill K. (1996)
10.1126/science.1152624 / Science by Corden J. L. (2007)
10.1074/jbc.M402754200 / J. Biol. Chem by Degot S. (2004)
10.1074/jbc.270.37.21524 / J. Biol. Chem by Duncan P. I. (1995)
10.1016/j.tig.2008.03.008 / Trends Genet by Egloff S. (2008)
10.1038/nrm1262 / Nat. Rev. Mol. Cell Biol by Gall J. G. (2003)
10.1002/ar.a.20336 / Anat. Rec. A Discov. Mol. Cell Evol. Biol by Hall L. L. (2006)
10.1073/pnas.92.25.11524 / Proc. Natl. Acad. Sci. USA by Hedley M. L. (1995)
10.1101/gad.14.12.1415 / Genes Dev by Hirose Y. (2000)
10.1083/jcb.126.4.877 / J. Cell Biol by Huang S. (1994)
10.1083/jcb.133.4.719 / J. Cell Biol by Huang S. (1996)
10.1016/0092-8674(93)90159-N / Cell by Jiminez-Garcia L. F. (1993)
10.1038/nature03159 / Nature by Kittler R. (2004)
10.1038/ncb1659 / Nat. Cell Biol by Kittler R. (2007)
10.1038/nrm1172 / Nat. Rev. Mol. Cell Biol by Lamond A. I. (2003)
10.1126/science.288.5470.1385 / Science by Lewis J. D. (2000)
10.1016/0092-8674(91)90185-2 / Cell by Li H. (1991)
10.1101/gad.1359305 / Genes Dev by Li X. (2005)
10.1002/j.1460-2075.1994.tb06906.x / EMBO J by Mermoud J. E. (1994)
10.1093/emboj/18.15.4308 / EMBO J by Mintz P. J. (1999)
10.1242/jcs.113.11.1841 / J. Cell Sci by Misteli T. (2000)
10.1016/j.cell.2007.01.028 / Cell by Misteli T. (2007)
10.1083/jcb.143.2.297 / J. Cell Biol by Misteli T. (1998)
10.1038/387523a0 / Nature by Misteli (1997)
10.1016/S1097-2765(01)80002-2 / Mol. Cell by Misteli T. (1999)
10.1083/jcb.124.3.249 / J. Cell Biol by O'Keefe R. T. (1994)
10.1091/mbc.e06-01-0036
10.1101/gad.1477006 / Genes Dev by Phatnani H. P. (2006)
10.1128/MCB.19.10.6991 / Mol. Cell. Biol by Prasad J. (1999)
10.1091/mbc.e02-10-0669
10.1261/rna.5207204 / RNA by Rosonina E. (2004)
10.1083/jcb.200107017 / J. Cell Biol by Sacco-Bubulya P. (2002)
10.1091/mbc.e04-03-0253
10.1111/j.1768-322X.1984.tb00215.x / Biol. Cell by Spector D. L. (1983)
10.1074/jbc.M101330200 / J. Biol. Chem by Sun C. T. (2001)
10.1111/j.1365-2443.2009.01324.x / Genes Cells by Takata H. (2009)
{'key': 'B45', 'first-page': '14', 'volume': '68', 'author': 'Thiry M.', 'year': '1995', 'journal-title': 'Eur. J. Cell Biol'} / Eur. J. Cell Biol by Thiry M. (1995)
10.1242/jcs.87.2.269 / J. Cell Sci by Turner B. M. (1987)
10.1006/geno.2000.6254 / Genomics by Wynn S. L. (2000)
10.1083/jcb.145.3.447 / J. Cell Biol by Yeakley J. M. (1999)

Dates

Type	When
Created	15 years, 7 months ago (Jan. 6, 2010, 10:19 p.m.)
Deposited	2 years, 3 months ago (May 29, 2023, 7:43 a.m.)
Indexed	5 days, 8 hours ago (Aug. 31, 2025, 7:19 p.m.)
Issued	15 years, 6 months ago (Feb. 15, 2010)
Published	15 years, 6 months ago (Feb. 15, 2010)
Published Print	15 years, 6 months ago (Feb. 15, 2010)

Funders 0

None

BibTeX

@article{Sharma_2010, title={Son Is Essential for Nuclear Speckle Organization and Cell Cycle Progression}, volume={21}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e09-02-0126}, DOI={10.1091/mbc.e09-02-0126}, number={4}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Sharma, Alok and Takata, Hideaki and Shibahara, Kei-ichi and Bubulya, Athanasios and Bubulya, Paula A.}, editor={Matera, A. Gregory}, year={2010}, month=feb, pages={650–663} }

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    {
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    },
    {
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      "DOI": "10.1083/jcb.200404120",
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    },
    {
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      "first-page": "225",
      "DOI": "10.1083/jcb.138.2.225",
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      "author": "Caceres J. F.",
      "year": "1997",
      "journal-title": "J. Cell Biol"
    },
    {
      "key": "B6",
      "doi-asserted-by": "crossref",
      "first-page": "2969",
      "DOI": "10.1128/MCB.25.8.2969-2980.2005",
      "volume": "25",
      "author": "Cazalla D.",
      "year": "2005",
      "journal-title": "Mol. Cell. Biol"
    },
    {
      "key": "B7",
      "doi-asserted-by": "crossref",
      "first-page": "105",
      "DOI": "10.1146/annurev.cellbio.20.010403.103738",
      "volume": "21",
      "author": "Cioce M.",
      "year": "2005",
      "journal-title": "Annu. Rev. Cell Dev. Biol"
    },
    {
      "key": "B8",
      "doi-asserted-by": "crossref",
      "first-page": "4431",
      "DOI": "10.1128/MCB.02235-06",
      "volume": "27",
      "author": "Cline E. G.",
      "year": "2007",
      "journal-title": "Mol. Cell. Biol"
    },
    {
      "key": "B9",
      "doi-asserted-by": "crossref",
      "first-page": "265",
      "DOI": "10.1002/j.1460-2075.1996.tb00357.x",
      "volume": "15",
      "author": "Colwill K.",
      "year": "1996",
      "journal-title": "EMBO J"
    },
    {
      "key": "B10",
      "doi-asserted-by": "crossref",
      "first-page": "1735",
      "DOI": "10.1126/science.1152624",
      "volume": "318",
      "author": "Corden J. L.",
      "year": "2007",
      "journal-title": "Science"
    },
    {
      "key": "B11",
      "doi-asserted-by": "crossref",
      "first-page": "33702",
      "DOI": "10.1074/jbc.M402754200",
      "volume": "279",
      "author": "Degot S.",
      "year": "2004",
      "journal-title": "J. Biol. Chem"
    },
    {
      "key": "B12",
      "doi-asserted-by": "crossref",
      "first-page": "21524",
      "DOI": "10.1074/jbc.270.37.21524",
      "volume": "270",
      "author": "Duncan P. I.",
      "year": "1995",
      "journal-title": "J. Biol. Chem"
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    {
      "key": "B13",
      "doi-asserted-by": "crossref",
      "first-page": "280",
      "DOI": "10.1016/j.tig.2008.03.008",
      "volume": "24",
      "author": "Egloff S.",
      "year": "2008",
      "journal-title": "Trends Genet"
    },
    {
      "key": "B14",
      "doi-asserted-by": "crossref",
      "first-page": "975",
      "DOI": "10.1038/nrm1262",
      "volume": "4",
      "author": "Gall J. G.",
      "year": "2003",
      "journal-title": "Nat. Rev. Mol. Cell Biol"
    },
    {
      "key": "B15",
      "doi-asserted-by": "crossref",
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      "DOI": "10.1002/ar.a.20336",
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      "doi-asserted-by": "crossref",
      "first-page": "11524",
      "DOI": "10.1073/pnas.92.25.11524",
      "volume": "92",
      "author": "Hedley M. L.",
      "year": "1995",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
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      "doi-asserted-by": "crossref",
      "first-page": "1415",
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      "year": "2000",
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    {
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      "doi-asserted-by": "crossref",
      "first-page": "877",
      "DOI": "10.1083/jcb.126.4.877",
      "volume": "126",
      "author": "Huang S.",
      "year": "1994",
      "journal-title": "J. Cell Biol"
    },
    {
      "key": "B19",
      "doi-asserted-by": "crossref",
      "first-page": "719",
      "DOI": "10.1083/jcb.133.4.719",
      "volume": "131",
      "author": "Huang S.",
      "year": "1996",
      "journal-title": "J. Cell Biol"
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    {
      "key": "B20",
      "doi-asserted-by": "crossref",
      "first-page": "47",
      "DOI": "10.1016/0092-8674(93)90159-N",
      "volume": "73",
      "author": "Jiminez-Garcia L. F.",
      "year": "1993",
      "journal-title": "Cell"
    },
    {
      "key": "B21",
      "doi-asserted-by": "crossref",
      "first-page": "1036",
      "DOI": "10.1038/nature03159",
      "volume": "432",
      "author": "Kittler R.",
      "year": "2004",
      "journal-title": "Nature"
    },
    {
      "key": "B22",
      "doi-asserted-by": "crossref",
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      "DOI": "10.1038/ncb1659",
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      "author": "Kittler R.",
      "year": "2007",
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      "doi-asserted-by": "crossref",
      "first-page": "605",
      "DOI": "10.1038/nrm1172",
      "volume": "4",
      "author": "Lamond A. I.",
      "year": "2003",
      "journal-title": "Nat. Rev. Mol. Cell Biol"
    },
    {
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      "doi-asserted-by": "crossref",
      "first-page": "1385",
      "DOI": "10.1126/science.288.5470.1385",
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    {
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      "first-page": "335",
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      "author": "Li H.",
      "year": "1991",
      "journal-title": "Cell"
    },
    {
      "key": "B26",
      "doi-asserted-by": "crossref",
      "first-page": "2705",
      "DOI": "10.1101/gad.1359305",
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      "author": "Li X.",
      "year": "2005",
      "journal-title": "Genes Dev"
    },
    {
      "key": "B27",
      "doi-asserted-by": "crossref",
      "first-page": "5679",
      "DOI": "10.1002/j.1460-2075.1994.tb06906.x",
      "volume": "13",
      "author": "Mermoud J. E.",
      "year": "1994",
      "journal-title": "EMBO J"
    },
    {
      "key": "B28",
      "doi-asserted-by": "crossref",
      "first-page": "4308",
      "DOI": "10.1093/emboj/18.15.4308",
      "volume": "18",
      "author": "Mintz P. J.",
      "year": "1999",
      "journal-title": "EMBO J"
    },
    {
      "key": "B29",
      "doi-asserted-by": "crossref",
      "first-page": "181",
      "DOI": "10.1242/jcs.113.11.1841",
      "volume": "113",
      "author": "Misteli T.",
      "year": "2000",
      "journal-title": "J. Cell Sci"
    },
    {
      "key": "B30",
      "doi-asserted-by": "crossref",
      "first-page": "787",
      "DOI": "10.1016/j.cell.2007.01.028",
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      "year": "2007",
      "journal-title": "Cell"
    },
    {
      "key": "B31",
      "doi-asserted-by": "crossref",
      "first-page": "297",
      "DOI": "10.1083/jcb.143.2.297",
      "volume": "143",
      "author": "Misteli T.",
      "year": "1998",
      "journal-title": "J. Cell Biol"
    },
    {
      "key": "B32",
      "doi-asserted-by": "crossref",
      "first-page": "523",
      "DOI": "10.1038/387523a0",
      "volume": "387",
      "author": "Misteli",
      "year": "1997",
      "journal-title": "Nature"
    },
    {
      "key": "B33",
      "doi-asserted-by": "crossref",
      "first-page": "697",
      "DOI": "10.1016/S1097-2765(01)80002-2",
      "volume": "3",
      "author": "Misteli T.",
      "year": "1999",
      "journal-title": "Mol. Cell"
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    {
      "key": "B34",
      "doi-asserted-by": "crossref",
      "first-page": "249",
      "DOI": "10.1083/jcb.124.3.249",
      "volume": "124",
      "author": "O'Keefe R. T.",
      "year": "1994",
      "journal-title": "J. Cell Biol"
    },
    {
      "key": "B35",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.e06-01-0036"
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      "first-page": "2922",
      "DOI": "10.1101/gad.1477006",
      "volume": "20",
      "author": "Phatnani H. P.",
      "year": "2006",
      "journal-title": "Genes Dev"
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      "first-page": "6991",
      "DOI": "10.1128/MCB.19.10.6991",
      "volume": "19",
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      "year": "1999",
      "journal-title": "Mol. Cell. Biol"
    },
    {
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      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.e02-10-0669"
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      "first-page": "581",
      "DOI": "10.1261/rna.5207204",
      "volume": "10",
      "author": "Rosonina E.",
      "year": "2004",
      "journal-title": "RNA"
    },
    {
      "key": "B40",
      "doi-asserted-by": "crossref",
      "first-page": "425",
      "DOI": "10.1083/jcb.200107017",
      "volume": "156",
      "author": "Sacco-Bubulya P.",
      "year": "2002",
      "journal-title": "J. Cell Biol"
    },
    {
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      "DOI": "10.1111/j.1768-322X.1984.tb00215.x",
      "volume": "49",
      "author": "Spector D. L.",
      "year": "1983",
      "journal-title": "Biol. Cell"
    },
    {
      "key": "B43",
      "doi-asserted-by": "crossref",
      "first-page": "24059",
      "DOI": "10.1074/jbc.M101330200",
      "volume": "276",
      "author": "Sun C. T.",
      "year": "2001",
      "journal-title": "J. Biol. Chem"
    },
    {
      "key": "B44",
      "doi-asserted-by": "crossref",
      "first-page": "975",
      "DOI": "10.1111/j.1365-2443.2009.01324.x",
      "volume": "14",
      "author": "Takata H.",
      "year": "2009",
      "journal-title": "Genes Cells"
    },
    {
      "key": "B45",
      "first-page": "14",
      "volume": "68",
      "author": "Thiry M.",
      "year": "1995",
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    },
    {
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      "doi-asserted-by": "crossref",
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