DOI: 10.1091/mbc.e06-02-0128. Identification of the Yeast R-SNARE Nyv1p as a Novel Longin Domain-containing Protein

Identification of the Yeast R-SNARE Nyv1p as a Novel Longin Domain-containing Protein

10.1091/mbc.e06-02-0128

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Using nuclear magnetic resonance spectroscopy, we establish that the N-terminal domain of the yeast vacuolar R-SNARE Nyv1p adopts a longin-like fold similar to those of Sec22b and Ykt6p. Nyv1p is sorted to the limiting membrane of the vacuole via the adaptor protein (AP)3 adaptin pathway, and we show that its longin domain is sufficient to direct transport to this location. In contrast, we found that the longin domains of Sec22p and Ykt6p were not sufficient to direct their localization. A YXXΦ-like adaptin-dependent sorting signal (Y31GTI34) unique to the longin domain of Nyv1p mediates interactions with the AP3 complex in vivo and in vitro. We show that amino acid substitutions to Y31GTI34 (Y31Q;I34Q) resulted in mislocalization of Nyv1p as well as reduced binding of the mutant protein to the AP3 complex. Although the sorting of Nyv1p to the limiting membrane of the vacuole is dependent upon the Y31GTI34 motif, and Y31 in particular, our findings with structure-based amino acid substitutions in the mu chain (Apm3p) of yeast AP3 suggest a mechanistically distinct role for this subunit in the recognition of YXXΦ-like sorting signals.

Bibliography

Wen, W., Chen, L., Wu, H., Sun, X., Zhang, M., & Banfield, D. K. (2006). Identification of the Yeast R-SNARE Nyv1p as a Novel Longin Domain-containing Protein. Molecular Biology of the Cell, 17(10), 4282â4299.

Authors 6

Wenyu Wen (first)
Lu Chen (additional)
Hao Wu (additional)
Xin Sun (additional)
Mingjie Zhang (additional)
David K. Banfield (additional)

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Dates

Type	When
Created	19 years, 1 month ago (July 19, 2006, 4:45 p.m.)
Deposited	6 years, 1 month ago (July 14, 2019, 7:22 a.m.)
Indexed	1 year, 1 month ago (July 7, 2024, 5:10 a.m.)
Issued	18 years, 10 months ago (Oct. 1, 2006)
Published	18 years, 10 months ago (Oct. 1, 2006)
Published Print	18 years, 10 months ago (Oct. 1, 2006)

Funders 0

None

BibTeX

@article{Wen_2006, title={Identification of the Yeast R-SNARE Nyv1p as a Novel Longin Domain-containing Protein}, volume={17}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e06-02-0128}, DOI={10.1091/mbc.e06-02-0128}, number={10}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Wen, Wenyu and Chen, Lu and Wu, Hao and Sun, Xin and Zhang, Mingjie and Banfield, David K.}, editor={Glick, Benjamin}, year={2006}, month=oct, pages={4282–4299} }

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