DOI: 10.1091/mbc.e04-08-0697. The Mitochondrial Inner Membrane Protein Mitofilin Controls Cristae Morphology

The Mitochondrial Inner Membrane Protein Mitofilin Controls Cristae Morphology

10.1091/mbc.e04-08-0697

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Mitochondria are complex organelles with a highly dynamic distribution and internal organization. Here, we demonstrate that mitofilin, a previously identified mitochondrial protein of unknown function, controls mitochondrial cristae morphology. Mitofilin is enriched in the narrow space between the inner boundary and the outer membranes, where it forms a homotypic interaction and assembles into a large multimeric protein complex. Down-regulation of mitofilin in HeLa cells by using specific small interfering RNA lead to decreased cellular proliferation and increased apoptosis, suggesting abnormal mitochondrial function. Although gross mitochondrial fission and fusion seemed normal, ultrastructural studies revealed disorganized mitochondrial inner membrane. Inner membranes failed to form tubular or vesicular cristae and showed as closely packed stacks of membrane sheets that fused intermittently, resulting in a complex maze of membranous network. Electron microscopic tomography estimated a substantial increase in inner:outer membrane ratio, whereas no cristae junctions were detected. In addition, mitochondria subsequently exhibited increased reactive oxygen species production and membrane potential. Although metabolic flux increased due to mitofilin deficiency, mitochondrial oxidative phosphorylation was not increased accordingly. We propose that mitofilin is a critical organizer of the mitochondrial cristae morphology and thus indispensable for normal mitochondrial function.

Bibliography

John, G. B., Shang, Y., Li, L., Renken, C., Mannella, C. A., Selker, J. M. L., Rangell, L., Bennett, M. J., & Zha, J. (2005). The Mitochondrial Inner Membrane Protein Mitofilin Controls Cristae Morphology. Molecular Biology of the Cell, 16(3), 1543â1554.

Authors 9

George B. John (first)
Yonglei Shang (additional)
Li Li (additional)
Christian Renken (additional)
Carmen A. Mannella (additional)
Jeanne M.L. Selker (additional)
Linda Rangell (additional)
Michael J. Bennett (additional)
Jiping Zha (additional)

References 50 Referenced 402

Dates

Type	When
Created	20 years, 7 months ago (Jan. 12, 2005, 8:24 p.m.)
Deposited	2 years, 3 months ago (May 1, 2023, 10:32 a.m.)
Indexed	14 hours, 38 minutes ago (Aug. 24, 2025, 7:08 p.m.)
Issued	20 years, 5 months ago (March 1, 2005)
Published	20 years, 5 months ago (March 1, 2005)
Published Print	20 years, 5 months ago (March 1, 2005)

Funders 0

None

BibTeX

@article{John_2005, title={The Mitochondrial Inner Membrane Protein Mitofilin Controls Cristae Morphology}, volume={16}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e04-08-0697}, DOI={10.1091/mbc.e04-08-0697}, number={3}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={John, George B. and Shang, Yonglei and Li, Li and Renken, Christian and Mannella, Carmen A. and Selker, Jeanne M.L. and Rangell, Linda and Bennett, Michael J. and Zha, Jiping}, year={2005}, month=mar, pages={1543–1554} }

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