DOI: 10.1091/mbc.e03-02-0074. The Role of the 3′ Untranslated Region in mRNA Sorting to the Vicinity of Mitochondria Is Conserved from Yeast to Human Cells

The Role of the 3′ Untranslated Region in mRNA Sorting to the Vicinity of Mitochondria Is Conserved from Yeast to Human Cells

10.1091/mbc.e03-02-0074

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

We recently demonstrated, using yeast DNA microarrays, that mRNAs of polysomes that coisolate with mitochondria code for a subset of mitochondrial proteins. The majority of these mRNAs encode proteins of prokaryotic origin. Herein, we show that a similar association occurs between polysomes and mitochondria in human cells. To determine whether mRNA transport machinery is conserved from yeast to human cells, we examined the subcellular localization of human OXA1 mRNA in yeast. Oxa1p is a key component in the biogenesis of mitochondrial inner membrane and is conserved from bacteria to eukaryotic organelles. The expression of human OXA1 cDNA partially restores the respiratory capacity of yeast oxa1–cells. In this study, we demonstrate that 1) OXA1 mRNAs are remarkably enriched in mitochondrion-bound polysomes purified from yeast and human cells; 2) the presence of the human OXA1 3′ untranslated region (UTR) is required for the function of the human Oxa1p inside yeast mitochondria; and 3) the accurate sorting of the human OXA1 mRNA to the vicinity of yeast mitochondria is due to the recognition by yeast proteins of the human 3′ UTR. Therefore, it seems that the recognition mechanism of OXA1 3′ UTR is conserved throughout evolution and is necessary for Oxa1p function.

Bibliography

Sylvestre, J., Margeot, A., Jacq, C., Dujardin, G., & Corral-Debrinski, M. (2003). The Role of the 3â² Untranslated Region in mRNA Sorting to the Vicinity of Mitochondria Is Conserved from Yeast to Human Cells. Molecular Biology of the Cell, 14(9), 3848â3856.

Authors 5

J. Sylvestre (first)
A. Margeot (additional)
C. Jacq (additional)
G. Dujardin (additional)
M. Corral-Debrinski (additional)

References 27 Referenced 117

Dates

Type	When
Created	22 years, 2 months ago (June 16, 2003, 8:35 p.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 7:06 p.m.)
Indexed	1 month, 4 weeks ago (July 2, 2025, 2:31 p.m.)
Issued	21 years, 11 months ago (Sept. 1, 2003)
Published	21 years, 11 months ago (Sept. 1, 2003)
Published Print	21 years, 11 months ago (Sept. 1, 2003)

Funders 0

None

BibTeX

@article{Sylvestre_2003, title={The Role of the 3′ Untranslated Region in mRNA Sorting to the Vicinity of Mitochondria Is Conserved from Yeast to Human Cells}, volume={14}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e03-02-0074}, DOI={10.1091/mbc.e03-02-0074}, number={9}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Sylvestre, J. and Margeot, A. and Jacq, C. and Dujardin, G. and Corral-Debrinski, M.}, year={2003}, month=sep, pages={3848–3856} }

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  "abstract": "<jats:p>We recently demonstrated, using yeast DNA microarrays, that mRNAs of polysomes that coisolate with mitochondria code for a subset of mitochondrial proteins. The majority of these mRNAs encode proteins of prokaryotic origin. Herein, we show that a similar association occurs between polysomes and mitochondria in human cells. To determine whether mRNA transport machinery is conserved from yeast to human cells, we examined the subcellular localization of human OXA1 mRNA in yeast. Oxa1p is a key component in the biogenesis of mitochondrial inner membrane and is conserved from bacteria to eukaryotic organelles. The expression of human OXA1 cDNA partially restores the respiratory capacity of yeast oxa1<jats:sup>\u2013</jats:sup>cells. In this study, we demonstrate that 1) OXA1 mRNAs are remarkably enriched in mitochondrion-bound polysomes purified from yeast and human cells; 2) the presence of the human OXA1 3\u2032 untranslated region (UTR) is required for the function of the human Oxa1p inside yeast mitochondria; and 3) the accurate sorting of the human OXA1 mRNA to the vicinity of yeast mitochondria is due to the recognition by yeast proteins of the human 3\u2032 UTR. Therefore, it seems that the recognition mechanism of OXA1 3\u2032 UTR is conserved throughout evolution and is necessary for Oxa1p function.</jats:p>",
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