STAM Proteins Bind Ubiquitinated Proteins on the Early Endosome via the VHS Domain and Ubiquitin-interacting Motif
10.1091/mbc.e02-12-0823
Crossref journal-article
American Society for Cell Biology (ASCB)
Molecular Biology of the Cell (1076)
Abstract

Conjugation with ubiquitin acts as a sorting signal for proteins in the endocytic and biosynthetic pathways at the endosome. Signal-transducing adaptor molecule (STAM) proteins, STAM1 and STAM2, are associated with hepatocyte growth factor-regulated substrate (Hrs) but their function remains unknown. Herein, we show that STAM proteins bind ubiquitin and ubiquitinated proteins and that the tandemly located VHS (Vps27/Hrs/STAM) domain and ubiquitin-interacting motif serve as the binding site(s). STAM proteins colocalize with Hrs on the early endosome. Overexpression of STAM proteins, but not their mutants lacking the ubiquitin-binding activity, causes the accumulation of ubiquitinated proteins and ligand-activated epidermal growth factor receptor on the early endosome. These results suggest that through interaction with ubiquitinated cargo proteins on the early endosome via the VHS domain and ubiquitin-interacting motif, STAM proteins participate in the sorting of cargo proteins for trafficking to the lysosome.

Bibliography

Mizuno, E., Kawahata, K., Kato, M., Kitamura, N., & Komada, M. (2003). STAM Proteins Bind Ubiquitinated Proteins on the Early Endosome via the VHS Domain and Ubiquitin-interacting Motif. Molecular Biology of the Cell, 14(9), 3675–3689.

Authors 5
  1. Emi Mizuno (first)
  2. Kensuke Kawahata (additional)
  3. Masaki Kato (additional)
  4. Naomi Kitamura (additional)
  5. Masayuki Komada (additional)
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Dates
Type When
Created 22 years, 2 months ago (June 16, 2003, 8:35 p.m.)
Deposited 4 years, 2 months ago (June 7, 2021, 11:30 a.m.)
Indexed 3 months, 1 week ago (May 28, 2025, 5:46 p.m.)
Issued 22 years ago (Sept. 1, 2003)
Published 22 years ago (Sept. 1, 2003)
Published Print 22 years ago (Sept. 1, 2003)
Funders 0

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@article{Mizuno_2003, title={STAM Proteins Bind Ubiquitinated Proteins on the Early Endosome via the VHS Domain and Ubiquitin-interacting Motif}, volume={14}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e02-12-0823}, DOI={10.1091/mbc.e02-12-0823}, number={9}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Mizuno, Emi and Kawahata, Kensuke and Kato, Masaki and Kitamura, Naomi and Komada, Masayuki}, year={2003}, month=sep, pages={3675–3689} }