Activity and Distribution of Paxillin, Focal Adhesion Kinase, and Cadherin Indicate Cooperative Roles during Zebrafish Morphogenesis
10.1091/mbc.e02-08-0537
Crossref journal-article
American Society for Cell Biology (ASCB)
Molecular Biology of the Cell (1076)
Abstract

We investigated the focal adhesion proteins paxillin and Fak, and the cell-cell adhesion protein cadherin in developing zebrafish (Danio rerio) embryos. Cadherins are expressed in presomitic mesoderm where they delineate cells. The initiation of somite formation coincides with an increase in the phosphorylation of Fak, and the accumulation of Fak, phosphorylated Fak, paxillin, and fibronectin at nascent somite boundaries. In the notochord, cadherins are expressed on cells during intercalation, and phosphorylated Fak accumulates in circumferential rings where the notochord cells contact laminin in the perichordal sheath. Subsequently, changes in the orientations of collagen fibers in the sheath suggest that Fak-mediated adhesion allows longitudinal expansion of the notochord, but not lateral expansion, resulting in notochord elongation. Novel observations showed that focal adhesion kinase and paxillin concentrate at sites of cell-cell adhesion in the epithelial enveloping layer and may associate with actin cytoskeleton at epithelial junctions containing cadherins. Fak is phosphorylated at these epithelial junctions but is not phosphorylated on Tyr397, implicating a noncanonical mechanism of regulation. These data suggest that Fak and paxillin may function in the integration of cadherin-based and integrin-based cell adhesion during the morphogenesis of the early zebrafish embryo.

Bibliography

Crawford, B. D., Henry, C. A., Clason, T. A., Becker, A. L., & Hille, M. B. (2003). Activity and Distribution of Paxillin, Focal Adhesion Kinase, and Cadherin Indicate Cooperative Roles during Zebrafish Morphogenesis. Molecular Biology of the Cell, 14(8), 3065–3081.

Authors 5
  1. Bryan D. Crawford (first)
  2. Clarissa A. Henry (additional)
  3. Todd A. Clason (additional)
  4. Amanda L. Becker (additional)
  5. Merrill B. Hille (additional)
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Dates
Type When
Created 22 years, 2 months ago (June 16, 2003, 8:35 p.m.)
Deposited 4 years, 2 months ago (June 7, 2021, 11:30 a.m.)
Indexed 2 months, 1 week ago (June 24, 2025, 12:46 p.m.)
Issued 22 years, 1 month ago (Aug. 1, 2003)
Published 22 years, 1 month ago (Aug. 1, 2003)
Published Print 22 years, 1 month ago (Aug. 1, 2003)
Funders 0

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@article{Crawford_2003, title={Activity and Distribution of Paxillin, Focal Adhesion Kinase, and Cadherin Indicate Cooperative Roles during Zebrafish Morphogenesis}, volume={14}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e02-08-0537}, DOI={10.1091/mbc.e02-08-0537}, number={8}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Crawford, Bryan D. and Henry, Clarissa A. and Clason, Todd A. and Becker, Amanda L. and Hille, Merrill B.}, year={2003}, month=aug, pages={3065–3081} }