DOI: 10.1091/mbc.e02-03-0175. Ordered Nucleation and Spreading of Silenced Chromatin in<i>Saccharomyces cerevisiae</i>

Ordered Nucleation and Spreading of Silenced Chromatin inSaccharomyces cerevisiae

10.1091/mbc.e02-03-0175

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

In Saccharomyces cerevisiae, silencing at theHM loci depends on Sir proteins, which are structural components of silenced chromatin. To explore the structure and assembly of silenced chromatin, the associations of Sir proteins with sequences across the HMR locus were examined by chromatin immunoprecipitation. In wild-type cells, Sir2p, Sir3p, and Sir4p were spread throughout and coincident with the silenced region atHMR. Sir1p, in contrast, associated only with theHMR-E silencer, consistent with its role in establishment but not maintenance of silencing. Sir4p was required for the association of other Sir proteins with silencers. In contrast, in the absence of Sir2p or Sir3p, partial assemblies of Sir proteins could form at silencers, where Sir protein assembly began. Spreading acrossHMR required Sir2p and Sir3p, as well as the deacetylase activity of Sir2p. These data support a model for the spreading of silenced chromatin involving cycles of nucleosome deacetylation by Sir2p followed by recruitment of additional Sir2p, Sir3p, and Sir4p to the newly deacetylated nucleosome. This model suggests mechanisms for boundary formation, and for maintenance and inheritance of silenced chromatin. The principles are generalizable to other types of heritable chromatin states.

Bibliography

RuschÃ©, L. N., Kirchmaier, A. L., & Rine, J. (2002). Ordered Nucleation and Spreading of Silenced Chromatin inSaccharomyces cerevisiae. Molecular Biology of the Cell, 13(7), 2207â2222.

Authors 3

Laura N. Rusché (first)
Ann L. Kirchmaier (additional)
Jasper Rine (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 28, 2002, 6:37 p.m.)
Deposited	1 year, 7 months ago (Jan. 6, 2024, 7:04 a.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:45 p.m.)
Issued	23 years, 1 month ago (July 1, 2002)
Published	23 years, 1 month ago (July 1, 2002)
Published Print	23 years, 1 month ago (July 1, 2002)

Funders 0

None

BibTeX

@article{Rusch__2002, title={Ordered Nucleation and Spreading of Silenced Chromatin inSaccharomyces cerevisiae}, volume={13}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.e02-03-0175}, DOI={10.1091/mbc.e02-03-0175}, number={7}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Rusché, Laura N. and Kirchmaier, Ann L. and Rine, Jasper}, editor={Drubin, David}, year={2002}, month=jul, pages={2207–2222} }

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