DOI: 10.1091/mbc.9.9.2393. Phosphorylation of Sic1, a Cyclin-dependent Kinase (Cdk) Inhibitor, by Cdk Including Pho85 Kinase Is Required for Its Prompt Degradation

Phosphorylation of Sic1, a Cyclin-dependent Kinase (Cdk) Inhibitor, by Cdk Including Pho85 Kinase Is Required for Its Prompt Degradation

10.1091/mbc.9.9.2393

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

In the yeast Saccharomyces cerevisiae, Sic1, an inhibitor of Clb-Cdc28 kinases, must be phosphorylated and degraded in G1for cells to initiate DNA replication, and Cln-Cdc28 kinase appears to be primarily responsible for phosphorylation of Sic1. The Pho85 kinase is a yeast cyclin-dependent kinase (Cdk), which is not essential for cell growth unless both CLN1 andCLN2 are absent. We demonstrate that Pho85, when complexed with Pcl1, a G1cyclin homologue, can phosphorylate Sic1 in vitro, and that Sic1 appears to be more stable inpho85Δ cells. Three consensus Cdk phosphorylation sites present in Sic1 are phosphorylated in vivo, and two of them are required for prompt degradation of the inhibitor. Pho85 and other G1Cdks appear to phosphorylate Sic1 at different sites in vivo. Thus at least two distinct Cdks can participate in phosphorylation of Sic1 and may therefore regulate progression through G1.

Bibliography

Nishizawa, M., Kawasumi, M., Fujino, M., & Toh-e, A. (1998). Phosphorylation of Sic1, a Cyclin-dependent Kinase (Cdk) Inhibitor, by Cdk Including Pho85 Kinase Is Required for Its Prompt Degradation. Molecular Biology of the Cell, 9(9), 2393â2405.

Authors 4

Masafumi Nishizawa (first)
Masaoki Kawasumi (additional)
Marie Fujino (additional)
Akio Toh-e (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:16 p.m.)
Deposited	5 years, 1 month ago (July 29, 2020, 11:24 a.m.)
Indexed	3 weeks, 1 day ago (Aug. 7, 2025, 4:54 p.m.)
Issued	26 years, 11 months ago (Sept. 1, 1998)
Published	26 years, 11 months ago (Sept. 1, 1998)
Published Print	26 years, 11 months ago (Sept. 1, 1998)

Funders 0

None

BibTeX

@article{Nishizawa_1998, title={Phosphorylation of Sic1, a Cyclin-dependent Kinase (Cdk) Inhibitor, by Cdk Including Pho85 Kinase Is Required for Its Prompt Degradation}, volume={9}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.9.9.2393}, DOI={10.1091/mbc.9.9.2393}, number={9}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Nishizawa, Masafumi and Kawasumi, Masaoki and Fujino, Marie and Toh-e, Akio}, editor={Yanagida, Mitsuhiro}, year={1998}, month=sep, pages={2393–2405} }

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  "abstract": "<jats:p>In the yeast Saccharomyces cerevisiae, Sic1, an inhibitor of Clb-Cdc28 kinases, must be phosphorylated and degraded in G<jats:sub>1</jats:sub>for cells to initiate DNA replication, and Cln-Cdc28 kinase appears to be primarily responsible for phosphorylation of Sic1. The Pho85 kinase is a yeast cyclin-dependent kinase (Cdk), which is not essential for cell growth unless both CLN1 andCLN2 are absent. We demonstrate that Pho85, when complexed with Pcl1, a G<jats:sub>1</jats:sub>cyclin homologue, can phosphorylate Sic1 in vitro, and that Sic1 appears to be more stable inpho85\u0394 cells. Three consensus Cdk phosphorylation sites present in Sic1 are phosphorylated in vivo, and two of them are required for prompt degradation of the inhibitor. Pho85 and other G<jats:sub>1</jats:sub>Cdks appear to phosphorylate Sic1 at different sites in vivo. Thus at least two distinct Cdks can participate in phosphorylation of Sic1 and may therefore regulate progression through G<jats:sub>1</jats:sub>.</jats:p>",
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