DOI: 10.1091/mbc.9.7.1833. Multiple Domains of Fission Yeast Cdc19p (MCM2) Are Required for Its Association with the Core MCM Complex

Multiple Domains of Fission Yeast Cdc19p (MCM2) Are Required for Its Association with the Core MCM Complex

10.1091/mbc.9.7.1833

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

The members of the MCM protein family are essential eukaryotic DNA replication factors that form a six-member protein complex. In this study, we use antibodies to four MCM proteins to investigate the structure of and requirements for the formation of fission yeast MCM complexes in vivo, with particular regard to Cdc19p (MCM2). Gel filtration analysis shows that the MCM protein complexes are unstable and can be broken down to subcomplexes. Using coimmunoprecipitation, we find that Mis5p (MCM6) and Cdc21p (MCM4) are tightly associated with one another in a core complex with which Cdc19p loosely associates. Assembly of Cdc19p with the core depends upon Cdc21p. Interestingly, there is no obvious change in Cdc19p-containing MCM complexes through the cell cycle. Using a panel of Cdc19p mutants, we find that multiple domains of Cdc19p are required for MCM binding. These studies indicate that MCM complexes in fission yeast have distinct substructures, which may be relevant for function.

Bibliography

Sherman, D. A., Pasion, S. G., & Forsburg, S. L. (1998). Multiple Domains of Fission Yeast Cdc19p (MCM2) Are Required for Its Association with the Core MCM Complex. Molecular Biology of the Cell, 9(7), 1833â1845.

Authors 3

Daniel A. Sherman (first)
Sally G. Pasion (additional)
Susan L. Forsburg (additional)

References 41 Referenced 44

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Dates

Type	When
Created	12 years, 2 months ago (July 2, 2013, 7:13 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:36 p.m.)
Indexed	1 year, 7 months ago (Jan. 12, 2024, 6:39 p.m.)
Issued	27 years, 2 months ago (July 1, 1998)
Published	27 years, 2 months ago (July 1, 1998)
Published Print	27 years, 2 months ago (July 1, 1998)

Funders 0

None

BibTeX

@article{Sherman_1998, title={Multiple Domains of Fission Yeast Cdc19p (MCM2) Are Required for Its Association with the Core MCM Complex}, volume={9}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.9.7.1833}, DOI={10.1091/mbc.9.7.1833}, number={7}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Sherman, Daniel A. and Pasion, Sally G. and Forsburg, Susan L.}, year={1998}, month=jul, pages={1833–1845} }

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