DOI: 10.1091/mbc.9.6.1479. Effects of Age on the Posttranscriptional Regulation of CCAAT/Enhancer Binding Protein α and CCAAT/Enhancer Binding Protein β Isoform Synthesis in Control and LPS-Treated Livers

Effects of Age on the Posttranscriptional Regulation of CCAAT/Enhancer Binding Protein α and CCAAT/Enhancer Binding Protein β Isoform Synthesis in Control and LPS-Treated Livers

10.1091/mbc.9.6.1479

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

The CCAAT/enhancer binding protein α (C/EBPα) and CCAAT/enhancer binding protein β (C/EBPβ) mRNAs are templates for the differential translation of several isoforms. Immunoblotting detects C/EBPαs with molecular masses of 42, 38, 30, and 20 kDa and C/EBPβs of 35, 20, and ∼8.5 kDa. The DNA-binding activities and pool levels of p42C/EBPαand p30C/EBPαin control nuclear extracts decrease significantly whereas the binding activity and protein levels of the 20-kDa isoforms increase dramatically with LPS treatment. Our studies suggest that the LPS response involves alternative translational initiation at specific in-frame AUGs, producing specific C/EBPα and C/EBPβ isoform patterns. We propose that alternative translational initiation occurs by a leaky ribosomal scanning mechanism. We find that nuclear extracts from normal aged mouse livers have decreased p42C/EBPαlevels and binding activity, whereas those of p20C/EBPαand p20C/EBPβare increased. However, translation of 42-kDa C/EBPα is not down-regulated on polysomes, suggesting that aging may affect its nuclear translocation. Furthermore, recovery of the C/EBPα- and C/EBPβ-binding activities and pool levels from an LPS challenge is delayed significantly in aged mouse livers. Thus, aged livers have altered steady-state levels of C/EBPα and C/EBPβ isoforms. This result suggests that normal aging liver exhibits characteristics of chronic stress and a severe inability to recover from an inflammatory challenge.

Bibliography

Hsieh, C.-C., Xiong, W., Xie, Q., Rabek, J. P., Scott, S. G., An, M. R., Reisner, P. D., Kuninger, D. T., & Papaconstantinou, J. (1998). Effects of Age on the Posttranscriptional Regulation of CCAAT/Enhancer Binding Protein Î± and CCAAT/Enhancer Binding Protein Î² Isoform Synthesis in Control and LPS-Treated Livers. Molecular Biology of the Cell, 9(6), 1479â1494.

Authors 9

Ching-Chyuan Hsieh (first)
Wei Xiong (additional)
Qizhi Xie (additional)
Jeffrey P. Rabek (additional)
Sheen G. Scott (additional)
Mi Ra An (additional)
Peter D. Reisner (additional)
David T. Kuninger (additional)
John Papaconstantinou (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:13 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:36 p.m.)
Indexed	2 months, 2 weeks ago (June 10, 2025, 2:45 p.m.)
Issued	27 years, 2 months ago (June 1, 1998)
Published	27 years, 2 months ago (June 1, 1998)
Published Print	27 years, 2 months ago (June 1, 1998)

Funders 0

None

BibTeX

@article{Hsieh_1998, title={Effects of Age on the Posttranscriptional Regulation of CCAAT/Enhancer Binding Protein α and CCAAT/Enhancer Binding Protein β Isoform Synthesis in Control and LPS-Treated Livers}, volume={9}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.9.6.1479}, DOI={10.1091/mbc.9.6.1479}, number={6}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Hsieh, Ching-Chyuan and Xiong, Wei and Xie, Qizhi and Rabek, Jeffrey P. and Scott, Sheen G. and An, Mi Ra and Reisner, Peter D. and Kuninger, David T. and Papaconstantinou, John}, year={1998}, month=jun, pages={1479–1494} }

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