DOI: 10.1091/mbc.9.6.1339. Heat Stress Activates Fission Yeast Spc1/StyI MAPK by a MEKK-Independent Mechanism

Heat Stress Activates Fission Yeast Spc1/StyI MAPK by a MEKK-Independent Mechanism

10.1091/mbc.9.6.1339

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Fission yeast Spc1/StyI MAPK is activated by many environmental insults including high osmolarity, oxidative stress, and heat shock. Spc1/StyI is activated by Wis1, a MAPK kinase (MEK), which is itself activated by Wik1/Wak1/Wis4, a MEK kinase (MEKK). Spc1/StyI is inactivated by the tyrosine phosphatases Pyp1 and Pyp2. Inhibition of Pyp1 was recently reported to play a crucial role in the oxidative stress and heat shock responses. These conclusions were based on three findings: 1) osmotic, oxidative, and heat stresses activate Spc1/StyI in wis4 cells; 2) oxidative stress and heat shock activate Spc1/StyI in cells that express Wis1AA, in which MEKK consensus phosphorylation sites were replaced with alanine; and 3) Spc1/StyI is maximally activated in Δpyp1 cells. Contrary to these findings, we report: 1) Spc1/StyI activation by osmotic stress is greatly reduced in wis4 cells; 2)wis1-AA and Δwis1 cells have identical phenotypes; and 3) all forms of stress activate Spc1/StyI inΔpyp1 cells. We also report that heat shock, but not osmotic or oxidative stress, activate Spc1 in wis1-DDcells, which express Wis1 protein that has the MEKK consensus phosphorylation sites replaced with aspartic acid. Thus osmotic and oxidative stress activate Spc1/StyI by a MEKK-dependent process, whereas heat shock activates Spc1/StyI by a novel mechanism that does not require MEKK activation or Pyp1 inhibition.

Bibliography

Shiozaki, K., Shiozaki, M., & Russell, P. (1998). Heat Stress Activates Fission Yeast Spc1/StyI MAPK by a MEKK-Independent Mechanism. Molecular Biology of the Cell, 9(6), 1339â1349.

Authors 3

Kazuhiro Shiozaki (first)
Mitsue Shiozaki (additional)
Paul Russell (additional)

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Dates

Type	When
Created	12 years, 2 months ago (July 2, 2013, 7:13 p.m.)
Deposited	3 years, 6 months ago (Feb. 26, 2022, 9:36 p.m.)
Indexed	3 hours, 2 minutes ago (Sept. 4, 2025, 10:15 a.m.)
Issued	27 years, 3 months ago (June 1, 1998)
Published	27 years, 3 months ago (June 1, 1998)
Published Print	27 years, 3 months ago (June 1, 1998)

Funders 0

None

BibTeX

@article{Shiozaki_1998, title={Heat Stress Activates Fission Yeast Spc1/StyI MAPK by a MEKK-Independent Mechanism}, volume={9}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.9.6.1339}, DOI={10.1091/mbc.9.6.1339}, number={6}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Shiozaki, Kazuhiro and Shiozaki, Mitsue and Russell, Paul}, year={1998}, month=jun, pages={1339–1349} }

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