DOI: 10.1091/mbc.9.6.1293. The<i>UNI3</i>Gene Is Required for Assembly of Basal Bodies of<i>Chlamydomonas</i>and Encodes δ-Tubulin, a New Member of the Tubulin Superfamily

TheUNI3Gene Is Required for Assembly of Basal Bodies ofChlamydomonasand Encodes δ-Tubulin, a New Member of the Tubulin Superfamily

10.1091/mbc.9.6.1293

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

We have cloned the UNI3 gene inChlamydomonas and find that it encodes a new member of the tubulin superfamily. Although Uni3p shares significant sequence identity with α-, β-, and γ-tubulins, there is a region of Uni3p that has no similarity to tubulins or other known proteins. Mutantuni3–1 cells assemble zero, one, or two flagella. Pedigree analysis suggests that flagellar number inuni3–1 cells is a function of the age of the cell. The uniflagellate uni3–1 cells show a positional phenotype; the basal body opposite the eyespot templates the single flagellum. A percentage of uni3–1 cells also fail to orient the cleavage furrow properly, and basal bodies have been implicated in the placement of cleavage furrows in Chlamydomonas. Finally when uni3–1 cells are observed by electron microscopy, doublet rather than triplet microtubules are observed at the proximal end of the basal bodies. We propose that the Uni3 tubulin is involved in both the function and cell cycle-dependent maturation of basal bodies/centrioles.

Bibliography

Dutcher, S. K., & Trabuco, E. C. (1998). TheUNI3Gene Is Required for Assembly of Basal Bodies ofChlamydomonasand Encodes Î´-Tubulin, a New Member of the Tubulin Superfamily. Molecular Biology of the Cell, 9(6), 1293â1308.

Authors 2

Susan K. Dutcher (first)
Emanuel C. Trabuco (additional)

References 69 Referenced 192

10.1016/S0022-2836(05)80360-2 / J. Mol. Biol. by Altschul S.F. (1990)
10.1093/nar/21.13.3097 / Nucleic Acids Res. by Bairoach A. (1993)
10.1007/BF01323254 / Protoplasma by Beech P.L. (1988)
10.1002/cm.970310402 / Cell Motil. Cytoskeleton by Burns R.G. (1995)
10.1006/excr.1997.3618 / Exp. Cell Res. by Callaini G. (1997)
10.1002/j.1460-2075.1989.tb08426.x / EMBO J. by Debuchy R. (1989)
10.1093/nar/12.1Part1.387 / Nucleic Acids Res. by Devereux J. (1984)
10.1093/genetics/141.3.945 / Genetics by Ehler L.L. (1995)
10.1083/jcb.124.1.19 / J. Cell Biol. by Félix M.-A. (1994)
10.1016/S0076-6879(96)66026-1 / Methods Enzymol. by Felsenstein J. (1996)
10.1073/pnas.86.17.6449 / Proc. Natl. Acad. Sci. USA by Fernández E. (1989)
10.1016/0092-8674(94)90389-1 / Cell by Ferris P.J. (1994)
10.1126/science.155.3760.279 / Science by Fitch W.M. (1967)
10.1128/MMBR.44.4.572-630.1980 / Microbiol. Rev. by Foster K.W. (1981)
10.1083/jcb.76.2.430 / J. Cell Biol. by Goodenough U.W. (1978)
10.1007/BF00309931 / Curr. Genet. by Gumpel N.J. (1994)
10.1006/geno.1994.1018 / Genomics by Henikoff S. (1994)
10.1242/jcs.94.2.273 / J. Cell Sci. by Holmes J.A. (1989)
10.1093/genetics/133.4.865 / Genetics by Holmes J.A. (1991)
10.1242/jcs.99.4.693 / J. Cell Sci. by Horio T. (1991)
10.1083/jcb.120.3.733 / J. Cell Biol. by Horst C.J. (1993)
10.1146/annurev.ge.21.120187.001355 / Annu. Rev. Genet. by Huffaker T.C. (1987)
10.1016/0092-8674(82)90436-6 / Cell by Huang B. (1982)
10.1242/jcs.106.1.209 / J. Cell Sci. by James S.W. (1993)
10.1083/jcb.113.2.339 / J. Cell Biol. by Johnson D.E. (1991)
10.1083/jcb.38.2.403 / J. Cell Biol. by Johnson U.G. (1968)
10.1093/oxfordjournals.molbev.a025576 / Mol. Biol. Evol. by Keeling P.J. (1996)
10.1016/0962-8924(96)20033-9 / Trends Cell Biol. by Keeling P.J. (1996)
10.1073/pnas.87.3.1228 / Proc. Natl. Acad. Sci. U.S.A. by Kindle K.L. (1990)
10.1083/jcb.136.1.177 / J. Cell Biol. by King S.J. (1997)
10.1083/jcb.110.5.1599 / J. Cell Biol. by Kochanski R.S. (1990)
10.1083/jcb.130.4.919 / J. Cell Biol. by Lange B. (1995)
10.1091/mbc.6.6.713
10.1093/genetics/128.3.549 / Genetics by Lux F.G. (1991)
10.1083/jcb.45.2.306 / J. Cell Biol. by Mahowald A.P. (1970)
10.1083/jcb.134.2.443 / J. Cell Biol. by Marschall L. (1996)
10.1002/jemt.1070240603 / Microsc. Res. Tech. by McDonald K. (1993)
10.1038/382710a0 / Nature by Mello C.C. (1996)
10.1073/pnas.94.10.5189 / Proc. Natl. Acad. Sci. U.S.A. by Miklos G.L. (1997)
10.2216/i0031-8884-21-4-427.1 / Phycologia by Moestrup Ø. (1982)
10.1083/jcb.130.5.1149 / J. Cell Biol. by Moritz M. (1995)
10.1091/mbc.8.4.607
10.1128/MCB.15.10.5762 / Mol. Cell. Biol. by Nelson J.A. (1995)
10.1038/338662a0 / Nature by Oakley C.E. (1989)
10.1016/0092-8674(90)90693-9 / Cell by Oakley B.R. (1990)
10.1016/1047-8477(92)90011-X / J. Struct. Biol. by Paintrand M. (1992)
10.1083/jcb.131.2.427 / J. Cell Biol. by Pazour G.J. (1995)
10.1073/pnas.85.8.2444 / Proc. Natl. Acad. Sci. U.S.A. by Pearson W.R. (1988)
10.1083/jcb.118.5.1163 / J. Cell Biol. by Porter M.E. (1992)
10.1093/genetics/120.1.109 / Genetics by Ranum L.P. W. (1988)
{'key': 'B59', 'first-page': '117', 'volume': '44', 'author': 'Reider C.L.', 'year': '1982', 'journal-title': 'Biol. Cell'} / Biol. Cell by Reider C.L. (1982)
{'key': 'B60', 'first-page': '383', 'volume': '13', 'author': 'Ringo D.L.', 'year': '1967', 'journal-title': 'J. Cell Biol.'} / J. Cell Biol. by Ringo D.L. (1967)
10.1002/cm.970180404 / Cell Motil. by Rüffer U. (1987)
10.1083/jcb.132.3.359 / J. Cell Biol. by Smith E. (1996)
10.1083/jcb.131.6.1775 / J. Cell Biol. by Sobel S.G. (1995)
10.1002/(SICI)1097-0134(199707)28:3<405::AID-PROT10>3.0.CO;2-L / Proteins by Sonnhammer E.L.L. (1997)
10.1083/jcb.134.2.429 / J. Cell Biol. by Spang A. (1996)
10.1016/0092-8674(94)90503-7 / Cell by Stearns T. (1994)
10.1016/0092-8674(91)90390-K / Cell by Stearns T. (1991)
10.1016/B978-0-12-395580-7.50013-7 / Formation and Fate of Cell Organelles by Stubblefield E. (1967)
10.1038/356037a0 / Nature by Sulston J. (1992)
10.1002/j.1460-2075.1995.tb06972.x / EMBO J. by Sunkel C.E. (1995)
10.1093/genetics/135.2.375 / Genetics by Tam L.-W. (1993)
10.1093/nar/22.22.4673 / Nucleic Acids Res. by Thompson J.D. (1994)
10.1242/jcs.108.3.1083 / J. Cell Sci. by Vassilev A. (1995)
10.1083/jcb.93.3.938 / J. Cell Biol. by Vorobjev I.A. (1982)
10.1007/BF01323255 / Protoplasma by Wetherbee R. (1988)
10.1128/MCB.4.12.2686 / Mol. Cell. Biol. by Youngblood J. (1984)
10.1038/378578a0 / Nature by Zheng Y. (1995)

Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:13 p.m.)
Deposited	3 years, 5 months ago (Feb. 26, 2022, 9:36 p.m.)
Indexed	2 weeks, 4 days ago (Aug. 5, 2025, 8:49 a.m.)
Issued	27 years, 2 months ago (June 1, 1998)
Published	27 years, 2 months ago (June 1, 1998)
Published Print	27 years, 2 months ago (June 1, 1998)

Funders 0

None

BibTeX

@article{Dutcher_1998, title={TheUNI3Gene Is Required for Assembly of Basal Bodies ofChlamydomonasand Encodes δ-Tubulin, a New Member of the Tubulin Superfamily}, volume={9}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.9.6.1293}, DOI={10.1091/mbc.9.6.1293}, number={6}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Dutcher, Susan K. and Trabuco, Emanuel C.}, year={1998}, month=jun, pages={1293–1308} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        5
      ]
    ],
    "date-time": "2025-08-05T12:49:06Z",
    "timestamp": 1754398146973
  },
  "reference-count": 69,
  "publisher": "American Society for Cell Biology (ASCB)",
  "issue": "6",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1998,
        6
      ]
    ]
  },
  "abstract": "<jats:p>We have cloned the UNI3 gene inChlamydomonas and find that it encodes a new member of the tubulin superfamily. Although Uni3p shares significant sequence identity with \u03b1-, \u03b2-, and \u03b3-tubulins, there is a region of Uni3p that has no similarity to tubulins or other known proteins. Mutantuni3\u20131 cells assemble zero, one, or two flagella. Pedigree analysis suggests that flagellar number inuni3\u20131 cells is a function of the age of the cell. The uniflagellate uni3\u20131 cells show a positional phenotype; the basal body opposite the eyespot templates the single flagellum. A percentage of uni3\u20131 cells also fail to orient the cleavage furrow properly, and basal bodies have been implicated in the placement of cleavage furrows in Chlamydomonas. Finally when uni3\u20131 cells are observed by electron microscopy, doublet rather than triplet microtubules are observed at the proximal end of the basal bodies. We propose that the Uni3 tubulin is involved in both the function and cell cycle-dependent maturation of basal bodies/centrioles.</jats:p>",
  "DOI": "10.1091/mbc.9.6.1293",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2013,
        7,
        2
      ]
    ],
    "date-time": "2013-07-02T23:13:12Z",
    "timestamp": 1372806792000
  },
  "page": "1293-1308",
  "source": "Crossref",
  "is-referenced-by-count": 192,
  "title": "The<i>UNI3</i>Gene Is Required for Assembly of Basal Bodies of<i>Chlamydomonas</i>and Encodes \u03b4-Tubulin, a New Member of the Tubulin Superfamily",
  "prefix": "10.1091",
  "volume": "9",
  "author": [
    {
      "given": "Susan K.",
      "family": "Dutcher",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Boulder, Colorado 80309-0347"
        }
      ]
    },
    {
      "given": "Emanuel C.",
      "family": "Trabuco",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Boulder, Colorado 80309-0347"
        }
      ]
    }
  ],
  "member": "1076",
  "reference": [
    {
      "key": "B1",
      "doi-asserted-by": "crossref",
      "first-page": "403",
      "DOI": "10.1016/S0022-2836(05)80360-2",
      "volume": "215",
      "author": "Altschul S.F.",
      "year": "1990",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "B2",
      "doi-asserted-by": "crossref",
      "first-page": "3097",
      "DOI": "10.1093/nar/21.13.3097",
      "volume": "21",
      "author": "Bairoach A.",
      "year": "1993",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "B3",
      "doi-asserted-by": "crossref",
      "first-page": "37",
      "DOI": "10.1007/BF01323254",
      "volume": "145",
      "author": "Beech P.L.",
      "year": "1988",
      "journal-title": "Protoplasma"
    },
    {
      "key": "B4",
      "doi-asserted-by": "crossref",
      "first-page": "255",
      "DOI": "10.1002/cm.970310402",
      "volume": "31",
      "author": "Burns R.G.",
      "year": "1995",
      "journal-title": "Cell Motil. Cytoskeleton"
    },
    {
      "key": "B6",
      "doi-asserted-by": "crossref",
      "first-page": "183",
      "DOI": "10.1006/excr.1997.3618",
      "volume": "234",
      "author": "Callaini G.",
      "year": "1997",
      "journal-title": "Exp. Cell Res."
    },
    {
      "key": "B7",
      "doi-asserted-by": "crossref",
      "first-page": "2803",
      "DOI": "10.1002/j.1460-2075.1989.tb08426.x",
      "volume": "8",
      "author": "Debuchy R.",
      "year": "1989",
      "journal-title": "EMBO J."
    },
    {
      "key": "B8",
      "doi-asserted-by": "crossref",
      "first-page": "387",
      "DOI": "10.1093/nar/12.1Part1.387",
      "volume": "12",
      "author": "Devereux J.",
      "year": "1984",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "B12",
      "doi-asserted-by": "crossref",
      "first-page": "945",
      "DOI": "10.1093/genetics/141.3.945",
      "volume": "141",
      "author": "Ehler L.L.",
      "year": "1995",
      "journal-title": "Genetics"
    },
    {
      "key": "B13",
      "doi-asserted-by": "crossref",
      "first-page": "19",
      "DOI": "10.1083/jcb.124.1.19",
      "volume": "124",
      "author": "F\u00e9lix M.-A.",
      "year": "1994",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B14",
      "doi-asserted-by": "crossref",
      "first-page": "418",
      "DOI": "10.1016/S0076-6879(96)66026-1",
      "volume": "266",
      "author": "Felsenstein J.",
      "year": "1996",
      "journal-title": "Methods Enzymol."
    },
    {
      "key": "B15",
      "doi-asserted-by": "crossref",
      "first-page": "6449",
      "DOI": "10.1073/pnas.86.17.6449",
      "volume": "86",
      "author": "Fern\u00e1ndez E.",
      "year": "1989",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "B16",
      "doi-asserted-by": "crossref",
      "first-page": "1136",
      "DOI": "10.1016/0092-8674(94)90389-1",
      "volume": "76",
      "author": "Ferris P.J.",
      "year": "1994",
      "journal-title": "Cell"
    },
    {
      "key": "B18",
      "doi-asserted-by": "crossref",
      "first-page": "279",
      "DOI": "10.1126/science.155.3760.279",
      "volume": "155",
      "author": "Fitch W.M.",
      "year": "1967",
      "journal-title": "Science"
    },
    {
      "key": "B19",
      "doi-asserted-by": "crossref",
      "first-page": "572",
      "DOI": "10.1128/MMBR.44.4.572-630.1980",
      "volume": "44",
      "author": "Foster K.W.",
      "year": "1981",
      "journal-title": "Microbiol. Rev."
    },
    {
      "key": "B20",
      "doi-asserted-by": "crossref",
      "first-page": "430",
      "DOI": "10.1083/jcb.76.2.430",
      "volume": "76",
      "author": "Goodenough U.W.",
      "year": "1978",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B21",
      "doi-asserted-by": "crossref",
      "first-page": "438",
      "DOI": "10.1007/BF00309931",
      "volume": "26",
      "author": "Gumpel N.J.",
      "year": "1994",
      "journal-title": "Curr. Genet."
    },
    {
      "key": "B23",
      "doi-asserted-by": "crossref",
      "first-page": "97",
      "DOI": "10.1006/geno.1994.1018",
      "volume": "19",
      "author": "Henikoff S.",
      "year": "1994",
      "journal-title": "Genomics"
    },
    {
      "key": "B24",
      "doi-asserted-by": "crossref",
      "first-page": "273",
      "DOI": "10.1242/jcs.94.2.273",
      "volume": "94",
      "author": "Holmes J.A.",
      "year": "1989",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "B26",
      "doi-asserted-by": "crossref",
      "first-page": "865",
      "DOI": "10.1093/genetics/133.4.865",
      "volume": "133",
      "author": "Holmes J.A.",
      "year": "1991",
      "journal-title": "Genetics"
    },
    {
      "key": "B27",
      "doi-asserted-by": "crossref",
      "first-page": "693",
      "DOI": "10.1242/jcs.99.4.693",
      "volume": "99",
      "author": "Horio T.",
      "year": "1991",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "B28",
      "doi-asserted-by": "crossref",
      "first-page": "733",
      "DOI": "10.1083/jcb.120.3.733",
      "volume": "120",
      "author": "Horst C.J.",
      "year": "1993",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B29",
      "doi-asserted-by": "crossref",
      "first-page": "259",
      "DOI": "10.1146/annurev.ge.21.120187.001355",
      "volume": "21",
      "author": "Huffaker T.C.",
      "year": "1987",
      "journal-title": "Annu. Rev. Genet."
    },
    {
      "key": "B30",
      "doi-asserted-by": "crossref",
      "first-page": "745",
      "DOI": "10.1016/0092-8674(82)90436-6",
      "volume": "29",
      "author": "Huang B.",
      "year": "1982",
      "journal-title": "Cell"
    },
    {
      "key": "B31",
      "doi-asserted-by": "crossref",
      "first-page": "209",
      "DOI": "10.1242/jcs.106.1.209",
      "volume": "106",
      "author": "James S.W.",
      "year": "1993",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "B32",
      "doi-asserted-by": "crossref",
      "first-page": "339",
      "DOI": "10.1083/jcb.113.2.339",
      "volume": "113",
      "author": "Johnson D.E.",
      "year": "1991",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B33",
      "doi-asserted-by": "crossref",
      "first-page": "403",
      "DOI": "10.1083/jcb.38.2.403",
      "volume": "38",
      "author": "Johnson U.G.",
      "year": "1968",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B34",
      "doi-asserted-by": "crossref",
      "first-page": "1297",
      "DOI": "10.1093/oxfordjournals.molbev.a025576",
      "volume": "3",
      "author": "Keeling P.J.",
      "year": "1996",
      "journal-title": "Mol. Biol. Evol."
    },
    {
      "key": "B35",
      "doi-asserted-by": "crossref",
      "first-page": "375",
      "DOI": "10.1016/0962-8924(96)20033-9",
      "volume": "6",
      "author": "Keeling P.J.",
      "year": "1996",
      "journal-title": "Trends Cell Biol."
    },
    {
      "key": "B36",
      "doi-asserted-by": "crossref",
      "first-page": "1228",
      "DOI": "10.1073/pnas.87.3.1228",
      "volume": "87",
      "author": "Kindle K.L.",
      "year": "1990",
      "journal-title": "Proc. Natl. Acad. Sci. U.S.A."
    },
    {
      "key": "B37",
      "doi-asserted-by": "crossref",
      "first-page": "177",
      "DOI": "10.1083/jcb.136.1.177",
      "volume": "136",
      "author": "King S.J.",
      "year": "1997",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B38",
      "doi-asserted-by": "crossref",
      "first-page": "1599",
      "DOI": "10.1083/jcb.110.5.1599",
      "volume": "110",
      "author": "Kochanski R.S.",
      "year": "1990",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B39",
      "doi-asserted-by": "crossref",
      "first-page": "919",
      "DOI": "10.1083/jcb.130.4.919",
      "volume": "130",
      "author": "Lange B.",
      "year": "1995",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B40",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.6.6.713"
    },
    {
      "key": "B41",
      "doi-asserted-by": "crossref",
      "first-page": "549",
      "DOI": "10.1093/genetics/128.3.549",
      "volume": "128",
      "author": "Lux F.G.",
      "year": "1991",
      "journal-title": "Genetics"
    },
    {
      "key": "B42",
      "doi-asserted-by": "crossref",
      "first-page": "306",
      "DOI": "10.1083/jcb.45.2.306",
      "volume": "45",
      "author": "Mahowald A.P.",
      "year": "1970",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B43",
      "doi-asserted-by": "crossref",
      "first-page": "443",
      "DOI": "10.1083/jcb.134.2.443",
      "volume": "134",
      "author": "Marschall L.",
      "year": "1996",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B44",
      "doi-asserted-by": "crossref",
      "first-page": "465",
      "DOI": "10.1002/jemt.1070240603",
      "volume": "24",
      "author": "McDonald K.",
      "year": "1993",
      "journal-title": "Microsc. Res. Tech."
    },
    {
      "key": "B46",
      "doi-asserted-by": "crossref",
      "first-page": "710",
      "DOI": "10.1038/382710a0",
      "volume": "382",
      "author": "Mello C.C.",
      "year": "1996",
      "journal-title": "Nature"
    },
    {
      "key": "B47",
      "doi-asserted-by": "crossref",
      "first-page": "5189",
      "DOI": "10.1073/pnas.94.10.5189",
      "volume": "94",
      "author": "Miklos G.L.",
      "year": "1997",
      "journal-title": "Proc. Natl. Acad. Sci. U.S.A."
    },
    {
      "key": "B48",
      "doi-asserted-by": "crossref",
      "first-page": "427",
      "DOI": "10.2216/i0031-8884-21-4-427.1",
      "volume": "21",
      "author": "Moestrup \u00d8.",
      "year": "1982",
      "journal-title": "Phycologia"
    },
    {
      "key": "B49",
      "doi-asserted-by": "crossref",
      "first-page": "1149",
      "DOI": "10.1083/jcb.130.5.1149",
      "volume": "130",
      "author": "Moritz M.",
      "year": "1995",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B50",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.8.4.607"
    },
    {
      "key": "B51",
      "doi-asserted-by": "crossref",
      "first-page": "5762",
      "DOI": "10.1128/MCB.15.10.5762",
      "volume": "15",
      "author": "Nelson J.A.",
      "year": "1995",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "B52",
      "doi-asserted-by": "crossref",
      "first-page": "662",
      "DOI": "10.1038/338662a0",
      "volume": "338",
      "author": "Oakley C.E.",
      "year": "1989",
      "journal-title": "Nature"
    },
    {
      "key": "B53",
      "doi-asserted-by": "crossref",
      "first-page": "1289",
      "DOI": "10.1016/0092-8674(90)90693-9",
      "volume": "61",
      "author": "Oakley B.R.",
      "year": "1990",
      "journal-title": "Cell"
    },
    {
      "key": "B54",
      "doi-asserted-by": "crossref",
      "first-page": "107",
      "DOI": "10.1016/1047-8477(92)90011-X",
      "volume": "108",
      "author": "Paintrand M.",
      "year": "1992",
      "journal-title": "J. Struct. Biol."
    },
    {
      "key": "B55",
      "doi-asserted-by": "crossref",
      "first-page": "427",
      "DOI": "10.1083/jcb.131.2.427",
      "volume": "131",
      "author": "Pazour G.J.",
      "year": "1995",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B56",
      "doi-asserted-by": "crossref",
      "first-page": "2444",
      "DOI": "10.1073/pnas.85.8.2444",
      "volume": "85",
      "author": "Pearson W.R.",
      "year": "1988",
      "journal-title": "Proc. Natl. Acad. Sci. U.S.A."
    },
    {
      "key": "B57",
      "doi-asserted-by": "crossref",
      "first-page": "1163",
      "DOI": "10.1083/jcb.118.5.1163",
      "volume": "118",
      "author": "Porter M.E.",
      "year": "1992",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B58",
      "doi-asserted-by": "crossref",
      "first-page": "109",
      "DOI": "10.1093/genetics/120.1.109",
      "volume": "120",
      "author": "Ranum L.P. W.",
      "year": "1988",
      "journal-title": "Genetics"
    },
    {
      "key": "B59",
      "first-page": "117",
      "volume": "44",
      "author": "Reider C.L.",
      "year": "1982",
      "journal-title": "Biol. Cell"
    },
    {
      "key": "B60",
      "first-page": "383",
      "volume": "13",
      "author": "Ringo D.L.",
      "year": "1967",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B61",
      "doi-asserted-by": "crossref",
      "first-page": "269",
      "DOI": "10.1002/cm.970180404",
      "volume": "18",
      "author": "R\u00fcffer U.",
      "year": "1987",
      "journal-title": "Cell Motil."
    },
    {
      "key": "B63",
      "doi-asserted-by": "crossref",
      "first-page": "359",
      "DOI": "10.1083/jcb.132.3.359",
      "volume": "132",
      "author": "Smith E.",
      "year": "1996",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B64",
      "doi-asserted-by": "crossref",
      "first-page": "1775",
      "DOI": "10.1083/jcb.131.6.1775",
      "volume": "131",
      "author": "Sobel S.G.",
      "year": "1995",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B65",
      "doi-asserted-by": "crossref",
      "first-page": "405",
      "DOI": "10.1002/(SICI)1097-0134(199707)28:3<405::AID-PROT10>3.0.CO;2-L",
      "volume": "28",
      "author": "Sonnhammer E.L.L.",
      "year": "1997",
      "journal-title": "Proteins"
    },
    {
      "key": "B66",
      "doi-asserted-by": "crossref",
      "first-page": "429",
      "DOI": "10.1083/jcb.134.2.429",
      "volume": "134",
      "author": "Spang A.",
      "year": "1996",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B67",
      "doi-asserted-by": "crossref",
      "first-page": "623",
      "DOI": "10.1016/0092-8674(94)90503-7",
      "volume": "76",
      "author": "Stearns T.",
      "year": "1994",
      "journal-title": "Cell"
    },
    {
      "key": "B68",
      "doi-asserted-by": "crossref",
      "first-page": "825",
      "DOI": "10.1016/0092-8674(91)90390-K",
      "volume": "65",
      "author": "Stearns T.",
      "year": "1991",
      "journal-title": "Cell"
    },
    {
      "key": "B69",
      "doi-asserted-by": "crossref",
      "first-page": "175",
      "DOI": "10.1016/B978-0-12-395580-7.50013-7",
      "volume": "6",
      "author": "Stubblefield E.",
      "year": "1967",
      "journal-title": "Formation and Fate of Cell Organelles"
    },
    {
      "key": "B70",
      "doi-asserted-by": "crossref",
      "first-page": "37",
      "DOI": "10.1038/356037a0",
      "volume": "356",
      "author": "Sulston J.",
      "year": "1992",
      "journal-title": "Nature"
    },
    {
      "key": "B71",
      "doi-asserted-by": "crossref",
      "first-page": "28",
      "DOI": "10.1002/j.1460-2075.1995.tb06972.x",
      "volume": "14",
      "author": "Sunkel C.E.",
      "year": "1995",
      "journal-title": "EMBO J."
    },
    {
      "key": "B72",
      "doi-asserted-by": "crossref",
      "first-page": "375",
      "DOI": "10.1093/genetics/135.2.375",
      "volume": "135",
      "author": "Tam L.-W.",
      "year": "1993",
      "journal-title": "Genetics"
    },
    {
      "key": "B73",
      "doi-asserted-by": "crossref",
      "first-page": "4673",
      "DOI": "10.1093/nar/22.22.4673",
      "volume": "22",
      "author": "Thompson J.D.",
      "year": "1994",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "B74",
      "doi-asserted-by": "crossref",
      "first-page": "1083",
      "DOI": "10.1242/jcs.108.3.1083",
      "volume": "108",
      "author": "Vassilev A.",
      "year": "1995",
      "journal-title": "J. Cell Sci."
    },
    {
      "key": "B75",
      "doi-asserted-by": "crossref",
      "first-page": "938",
      "DOI": "10.1083/jcb.93.3.938",
      "volume": "98",
      "author": "Vorobjev I.A.",
      "year": "1982",
      "journal-title": "J. Cell Biol."
    },
    {
      "key": "B76",
      "doi-asserted-by": "crossref",
      "first-page": "47",
      "DOI": "10.1007/BF01323255",
      "volume": "145",
      "author": "Wetherbee R.",
      "year": "1988",
      "journal-title": "Protoplasma"
    },
    {
      "key": "B77",
      "doi-asserted-by": "crossref",
      "first-page": "2686",
      "DOI": "10.1128/MCB.4.12.2686",
      "volume": "4",
      "author": "Youngblood J.",
      "year": "1984",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "B78",
      "doi-asserted-by": "crossref",
      "first-page": "578",
      "DOI": "10.1038/378578a0",
      "volume": "378",
      "author": "Zheng Y.",
      "year": "1995",
      "journal-title": "Nature"
    }
  ],
  "container-title": "Molecular Biology of the Cell",
  "original-title": [],
  "language": "en",
  "deposited": {
    "date-parts": [
      [
        2022,
        2,
        27
      ]
    ],
    "date-time": "2022-02-27T02:36:14Z",
    "timestamp": 1645929374000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.molbiolcell.org/doi/10.1091/mbc.9.6.1293"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1998,
        6
      ]
    ]
  },
  "references-count": 69,
  "journal-issue": {
    "issue": "6",
    "published-print": {
      "date-parts": [
        [
          1998,
          6
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1091/mbc.9.6.1293"
  ],
  "URL": "http://dx.doi.org/10.1091/mbc.9.6.1293",
  "relation": {},
  "ISSN": [
    "1059-1524",
    "1939-4586"
  ],
  "subject": [],
  "container-title-short": "MBoC",
  "published": {
    "date-parts": [
      [
        1998,
        6
      ]
    ]
  }
}