DOI: 10.1091/mbc.9.5.977. <i>Saccharomyces cerevisiae</i>Cells with Defective Spindle Pole Body Outer Plaques Accomplish Nuclear Migration via Half-Bridge

Saccharomyces cerevisiaeCells with Defective Spindle Pole Body Outer Plaques Accomplish Nuclear Migration via Half-Bridge–organized Microtubules

10.1091/mbc.9.5.977

Crossref journal-article

American Society for Cell Biology (ASCB)

Molecular Biology of the Cell (1076)

Abstract

Cnm67p, a novel yeast protein, localizes to the microtubule organizing center, the spindle pole body (SPB). Deletion ofCNM67 (YNL225c) frequently results in spindle misorientation and impaired nuclear migration, leading to the generation of bi- and multinucleated cells (40%). Electron microscopy indicated that CNM67 is required for proper formation of the SPB outer plaque, a structure that nucleates cytoplasmic (astral) microtubules. Interestingly, cytoplasmic microtubules that are essential for spindle orientation and nuclear migration are still present in cnm67Δ1 cells that lack a detectable outer plaque. These microtubules are attached to the SPB half- bridge throughout the cell cycle. This interaction presumably allows for low-efficiency nuclear migration and thus provides a rescue mechanism in the absence of a functional outer plaque. AlthoughCNM67 is not strictly required for mitosis, it is essential for sporulation. Time-lapse microscopy ofcnm67Δ1 cells with green fluorescent protein (GFP)-labeled nuclei indicated that CNM67 is dispensable for nuclear migration (congression) and nuclear fusion during conjugation. This is in agreement with previous data, indicating that cytoplasmic microtubules are organized by the half-bridge during mating.

Bibliography

Brachat, A., Kilmartin, J. V., Wach, A., & Philippsen, P. (1998). Saccharomyces cerevisiaeCells with Defective Spindle Pole Body Outer Plaques Accomplish Nuclear Migration via Half-Bridgeâorganized Microtubules. Molecular Biology of the Cell, 9(5), 977â991.

Authors 4

Arndt Brachat (first)
John V. Kilmartin (additional)
Achim Wach (additional)
Peter Philippsen (additional)

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Dates

Type	When
Created	12 years, 1 month ago (July 2, 2013, 7:13 p.m.)
Deposited	3 years, 5 months ago (Feb. 26, 2022, 9:36 p.m.)
Indexed	1 month, 2 weeks ago (July 2, 2025, 2:15 p.m.)
Issued	27 years, 3 months ago (May 1, 1998)
Published	27 years, 3 months ago (May 1, 1998)
Published Print	27 years, 3 months ago (May 1, 1998)

Funders 0

None

BibTeX

@article{Brachat_1998, title={Saccharomyces cerevisiaeCells with Defective Spindle Pole Body Outer Plaques Accomplish Nuclear Migration via Half-Bridge–organized Microtubules}, volume={9}, ISSN={1939-4586}, url={http://dx.doi.org/10.1091/mbc.9.5.977}, DOI={10.1091/mbc.9.5.977}, number={5}, journal={Molecular Biology of the Cell}, publisher={American Society for Cell Biology (ASCB)}, author={Brachat, Arndt and Kilmartin, John V. and Wach, Achim and Philippsen, Peter}, year={1998}, month=may, pages={977–991} }

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  "abstract": "<jats:p>Cnm67p, a novel yeast protein, localizes to the microtubule organizing center, the spindle pole body (SPB). Deletion ofCNM67 (YNL225c) frequently results in spindle misorientation and impaired nuclear migration, leading to the generation of bi- and multinucleated cells (40%). Electron microscopy indicated that CNM67 is required for proper formation of the SPB outer plaque, a structure that nucleates cytoplasmic (astral) microtubules. Interestingly, cytoplasmic microtubules that are essential for spindle orientation and nuclear migration are still present in cnm67\u03941 cells that lack a detectable outer plaque. These microtubules are attached to the SPB half- bridge throughout the cell cycle. This interaction presumably allows for low-efficiency nuclear migration and thus provides a rescue mechanism in the absence of a functional outer plaque. AlthoughCNM67 is not strictly required for mitosis, it is essential for sporulation. Time-lapse microscopy ofcnm67\u03941 cells with green fluorescent protein (GFP)-labeled nuclei indicated that CNM67 is dispensable for nuclear migration (congression) and nuclear fusion during conjugation. This is in agreement with previous data, indicating that cytoplasmic microtubules are organized by the half-bridge during mating.</jats:p>",
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